LOCUS       V035597                10667 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035597
VERSION     V035597
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             3181..3234
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             3235..4758
                     /label="SIRPA(NM_001330728)"
                     /note="SIRPA(NM_001330728)"
                     /gene="SIRPA"
     promoter        4826..5037
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5050..5318
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5347..5356
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5377..6039
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6109..6162
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6163..6762
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6763..7350
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7424..7657
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7729..7850
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7869..8004
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8042..8058)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8066..8082
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8090..8120)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8135..8156
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8444..9032)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9203..10063)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10064..10168)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10642..10658
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc agatcccaca ggcgccctgg
     2341 ccagtcgtct gggcggtgct acaactgggc tggcggccag gatggttctt agactcccca
     2401 gacaggccct ggaacccccc caccttctcc ccagccctgc tcgtggtgac cgaaggggac
     2461 aacgccacct tcacctgcag cttctccaac acatcggaga gcttcgtgct aaactggtac
     2521 cgcatgagcc ccagcaacca gacggacaag ctggccgcct tccccgagga ccgcagccag
     2581 cccggccagg actgccgctt ccgtgtcaca caactgccca acgggcgtga cttccacatg
     2641 agcgtggtca gggcccggcg caatgacagc ggcacctacc tctgtggggc catctccctg
     2701 gcccccaagg cgcagatcaa agagagcctg cgggcagagc tcagggtgac agagagaagg
     2761 gcagaagtgc ccacagccca ccccagcccc tcacccaggc cagccggcca gttccaaacc
     2821 ctggtggttg gtgtcgtggg cggcctgctg ggcagcctgg tgctgctagt ctgggtcctg
     2881 gccgtcatct gctcccgggc cgcacgaggg acaataggag ccaggcgcac cggccagccc
     2941 ctgaaggagg acccctcagc cgtgcctgtg ttctctgtgg actatgggga gctggatttc
     3001 cagtggcgag agaagacccc ggagcccccc gtgccctgtg tccctgagca gacggagtat
     3061 gccaccattg tctttcctag cggaatgggc acctcatccc ccgcccgcag gggctcagct
     3121 gacggccctc ggagtgccca gccactgagg cctgaggatg gacactgctc ttggcccctc
     3181 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatggag
     3241 cccgccggcc cggcccccgg ccgcctcggg ccgctgctct gcctgctgct cgccgcgtcc
     3301 tgcgcctggt caggagtggc gggtgaggag gagctgcagg tgattcagcc tgacaagtcc
     3361 gtgttggttg cagctggaga gacagccact ctgcgctgca ctgcgacctc tctgatccct
     3421 gtggggccca tccagtggtt cagaggagct ggaccaggcc gggaattaat ctacaatcaa
     3481 aaagaaggcc acttcccccg ggtaacaact gtttcagacc tcacaaagag aaacaacatg
     3541 gacttttcca tccgcatcgg taacatcacc ccagcagatg ccggcaccta ctactgtgtg
     3601 aagttccgga aagggagccc cgatgacgtg gagtttaagt ctggagcagg cactgagctg
     3661 tctgtgcgcg ccaaaccctc tgcccccgtg gtatcgggcc ctgcggcgag ggccacacct
     3721 cagcacacag tgagcttcac ctgcgagtcc cacggcttct cacccagaga catcaccctg
     3781 aaatggttca aaaatgggaa tgagctctca gacttccaga ccaacgtgga ccccgtagga
     3841 gagagcgtgt cctacagcat ccacagcaca gccaaggtgg tgctgacccg cgaggacgtt
     3901 cactctcaag tcatctgcga ggtggcccac gtcaccttgc agggggaccc tcttcgtggg
     3961 actgccaact tgtctgagac catccgagtt ccacccacct tggaggttac tcaacagccc
     4021 gtgagggcag agaaccaggt gaatgtcacc tgccaggtga ggaagttcta cccccagaga
     4081 ctacagctga cctggttgga gaatggaaac gtgtcccgga cagaaacggc ctcaaccgtt
     4141 acagagaaca aggatggtac ctacaactgg atgagctggc tcctggtgaa tgtatctgcc
     4201 cacagggatg atgtgaagct cacctgccag gtggagcatg acgggcagcc agcggtcagc
     4261 aaaagccatg acctgaaggt ctcagcccac ccgaaggagc agggctcaaa taccgccgct
     4321 gagaacactg gatctaatga acggaacatc tatattgtgg tgggtgtggt gtgcaccttg
     4381 ctggtggccc tactgatggc ggccctctac ctcgtccgaa tcagacagaa gaaagcccag
     4441 ggctccactt cttctacaag gttgcatgag cccgagaaga atgccagaga aataacacag
     4501 gtacagtcct tggacacaaa tgatatcaca tatgcagacc tgaacctgcc caaggggaag
     4561 aagcctgctc cccaggctgc ggagcccaac aaccacacgg agtatgccag cattcagacc
     4621 agcccgcagc ccgcgtcgga ggacaccctc acctatgctg acctggacat ggtccacctc
     4681 aaccggaccc ccaagcagcc ggcccccaag cctgagccgt ccttctcaga gtacgccagc
     4741 gtccaggtcc cgaggaagtg atgaggatcc ctgcgatatc cagcgcggcc gcgaaggatc
     4801 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4861 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     4921 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     4981 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     5041 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     5101 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     5161 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     5221 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     5281 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     5341 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     5401 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     5461 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     5521 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     5581 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     5641 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     5701 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     5761 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     5821 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     5881 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     5941 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     6001 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     6061 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     6121 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     6181 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     6241 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6301 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6361 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6421 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6481 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6541 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6601 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6661 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     6721 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     6781 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6841 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6901 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6961 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7021 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7081 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7141 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     7201 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7261 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg
     7321 agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     7381 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7441 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7501 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7561 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7621 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7681 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     7741 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7801 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7861 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7921 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7981 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     8041 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8101 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8161 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8221 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8281 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8341 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8401 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8461 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8521 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8581 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8641 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8701 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8761 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8821 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8881 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8941 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9001 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9061 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9121 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9181 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9241 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9301 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9361 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9421 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9481 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9541 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9601 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9661 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9721 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9781 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9841 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9901 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9961 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10021 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10081 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10141 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    10201 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    10261 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    10321 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    10381 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    10441 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    10501 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    10561 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    10621 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
