LOCUS       V034082                10581 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034082
VERSION     V034082
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5427
                     /label="SIPA1(NM_153253)"
                     /note="SIPA1(NM_153253)"
                     /gene="SIPA1"
     CDS             5434..5499
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5543..5754
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5767..6035
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6070..6669
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6676..7264
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7338..7571
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7643..7764
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7783..7918
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7956..7972)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7980..7996
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8004..8034)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8049..8070
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8358..8946)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9117..9977)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9978..10082)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10556..10572
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcccatg tgggccggcg gtgtggggag ccctcggcgg
     2341 ggcatggccc ctgcgtccac agatgacctc tttgcccgca agctgcgcca gccagcaagg
     2401 cccccgctga caccgcacac cttcgagccg aggccagtcc ggggcccact cctgcgcagc
     2461 ggcagcgatg caggcgaggc caggcccccc acgccagcca gcccccgtgc ccgtgcccac
     2521 agccacgaag aggccagccg acctgcagcc acttccaccc ggctcttcac tgacccgctg
     2581 gcactgctgg ggctgccagc agaggaacca gagcctgcct tcccaccagt gcttgagcct
     2641 cgatggtttg cccactatga cgtgcaaagc ctgctctttg attgggctcc gaggtctcag
     2701 gggatgggga gccactcaga ggccagctct gggaccctgg cttcagccga ggaccaggct
     2761 gccagctcgg acctgctgca tggggcacct ggctttgtgt gtgagctcgg gggtgagggt
     2821 gagctaggcc tgggtggacc agcatcccca cctgtgcccc ctgcactgcc caacgcggcc
     2881 gtgtccatcc tggaggagcc acagaaccga acctcggcct acagcctgga gcacgcagac
     2941 ctgggtgctg gctactaccg caaatacttc tatggcaaag aacatcagaa cttcttcggg
     3001 atggacgagt cgctgggccc ggtggcagtg agcctgcggc gggaggagaa ggagggcagc
     3061 ggagggggca ccctgcacag ctaccgcgtc atcgtgcgga ccacgcagct ccggacactc
     3121 cgtggcacca tctcggagga cgcgctgccg ccggggcccc cacggggtct gtccccaagg
     3181 aaacttctgg agcacgtggc gccgcagctg agccccagct gcctgcgcct gggctcagct
     3241 tcacccaagg taccacggac gctgctcaca ctggatgagc aagtgctgag cttccaacgc
     3301 aaggtgggca tcctgtactg ccgggcgggc cagggctcgg aggaggagat gtacaacaac
     3361 caggaggcgg gaccggcctt catgcagttt ctcaccttgc tgggcgatgt ggtgcggctc
     3421 aaaggctttg agagttaccg ggcccagcta gacaccaaaa cggattccac aggcacgcac
     3481 tccctctaca ccacatacca ggaccacgag atcatgttcc acgtgtccac gatgctgcct
     3541 tacaccccta ataaccagca gcagctcctc cggaagcgcc acattggcaa cgacattgtg
     3601 accatcgtgt tccaggagcc tggcagcaag cccttctgcc ccaccaccat ccgctcgcac
     3661 ttccagcacg tgttcctagt ggtgcgggca cacacaccct gcacgccaca caccacctac
     3721 agggtggccg tgagccgcac ccaggacacc cctgccttcg ggccagctct gcctgctggc
     3781 ggaggcccct tcgcagccaa cgccgacttc cgggccttcc tgctggccaa agcgctgaat
     3841 ggtgagcagg cggccggcca cgcgcgccag ttccacgcca tggccacgcg cacccgccag
     3901 cagtacctgc aagacctggc caccaacgag gtgaccacta cgtcgctgga ctcggcttca
     3961 cgcttcggcc tgccctccct gggtgggagg cgccgggcgg cccctcgggg cccaggcgcc
     4021 gagctgcagg cagcgggctc actggtgtgg ggagtgcgcg cggcgcccgg ggcgcgggtc
     4081 gccgccgggg ctcaggcgag cggccccgaa ggcatcgagg tgccctgcct gctgggcatc
     4141 tcggccgagg ctctggtgct ggtggcgccg cgcgacggcc gcgtagtgtt caattgcgcc
     4201 tgtcgcgacg tgctggcctg gaccttctcc gagcagcagc tggacctgta ccacggccgc
     4261 ggggaggcga tcacgctgcg cttcgacggg tcccccggcc aagccgtggg cgaggtggtg
     4321 gcgcgcctgc agctggtgag ccgtggctgc gagacccgcg agctggcgct gccccgcgac
     4381 ggtcaaggcc gcctgggctt cgaggtggac gccgagggat tcgtcacgca cgtggagcgc
     4441 ttcacattcg ccgagacggc ggggctgcgg cccggggcgc gcctcctgcg cgtgtgcggc
     4501 cagactctgc ccagcctccg gcccgaggcc gctgcccagc tcctgcgctc ggcgcccaag
     4561 gtctgcgtca ccgtcctgcc ccccgacgag agcggccggc cccgcaggag tttttcggag
     4621 ctgtacacgc tgtcgctgca ggagcctagc cggcgggggg ccccagatcc tgtgcaggat
     4681 gaggtccagg gggtgaccct gctgcccacc acaaagcagc tgctgcacct gtgcctgcaa
     4741 gatggtggca gtcctccagg gcctggggat ctggccgagg agaggactga gttcctgcac
     4801 agccagaact cgctgtcacc acgcagctct ctgtcggatg aggccccagt cctgcccaac
     4861 accaccccgg acctcctcct ggccaccaca gccaagccat cagtacccag tgctgacagt
     4921 gagacacccc tgacccagga caggccaggc agtcccagtg gctctgagga caagggcaac
     4981 ccggcgccgg agctgagggc ctcctttctg ccacgtacct tgtctctgcg gaactccatc
     5041 agcaggatca tgtcggaggc gggcagtggg accctggagg acgagtggca ggccatctcg
     5101 gagattgcct ctacttgcaa caccattctg gagtcgctgt cccgagaggg acagcccatc
     5161 ccagagagtg gagaccctaa gggaactcca aaatctgatg ctgagccaga gcctgggaac
     5221 ctctcagaga aggtctctca cttggagtcc atgctcagga agctgcagga ggacctgcag
     5281 aaggagaagg cggacagggc ggccctggag gaggaggtgc ggagcctgag acacaacaac
     5341 cggcggctgc aggcggagtc tgagagtgca gccacacgcc tcctcctggc ctccaagcag
     5401 ctgggctcac ccaccgccga cctggccgga tccgactaca aagaccatga cggtgattat
     5461 aaagatcatg acatcgacta caaggatgac gatgacaagt gagcggccgc aaggatctgc
     5521 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     5581 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     5641 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     5701 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     5761 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     5821 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     5881 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     5941 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     6001 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctaccccg
     6061 ggcggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6121 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6181 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6241 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6301 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6361 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6421 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6481 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6541 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     6601 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     6661 ggtgcctgag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6721 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6781 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6841 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6901 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6961 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7021 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     7081 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7141 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7201 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7261 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7321 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt
     7381 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7441 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7501 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7561 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7621 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7681 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7741 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7801 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7861 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7921 gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7981 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8041 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8101 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8161 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8221 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8281 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8341 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8401 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8461 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8521 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8581 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8641 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8701 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8761 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     8821 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8881 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8941 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9001 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9061 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9121 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9181 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9241 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9301 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9361 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9421 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9481 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9541 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9601 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9661 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9721 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9781 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9841 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9901 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9961 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10021 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10081 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
    10141 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
    10201 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
    10261 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
    10321 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
    10381 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
    10441 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    10501 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    10561 acgacggcca gtgccaagct g
//
