LOCUS       V042959                12240 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042959
VERSION     V042959
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..6259
                     /label="SIK3(NM_025164)"
                     /note="SIK3(NM_025164)"
                     /gene="SIK3"
     CDS             6266..6355
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        6399..6610
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6623..6891
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6920..6929
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6950..7612
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7682..7735
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7736..8335
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8336..8923
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8997..9230
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9302..9423
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9442..9577
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9615..9631)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9639..9655
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9663..9693)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9708..9729
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10017..10605)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10776..11636)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11637..11741)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12215..12231
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg cggcggcggc ggcgagcgga gctggcgggg ctgccggggc
     2341 cgggactggg ggagccgggc ccgcgggccg cctgctgcct ccgcccgcgc cggggtcccc
     2401 agccgccccc gctgccgtgt cccctgcggc cggccagccg cgtcccccag ccccggcctc
     2461 ccgcggaccc atgcccgccc gtatcggcta ctacgagatc gaccgcacca tcggcaaggg
     2521 caacttcgcg gtggtcaagc gggccacgca cctcgtcacc aaggccaagg ttgctatcaa
     2581 gatcatagat aagacccagc tggatgaaga aaacttgaag aagattttcc gggaagttca
     2641 aattatgaag atgctttgcc acccccatat catcaggctc taccaggtta tggagacaga
     2701 acggatgatt tatctggtga cagaatatgc tagtggaggg gaaatatttg accacctggt
     2761 ggcccatggt agaatggcag aaaaggaggc acgtcggaag ttcaaacaga tcgtcacagc
     2821 tgtctatttt tgtcactgtc ggaacattgt tcatcgtgat ttaaaagctg aaaatttact
     2881 tctggatgcc aatctgaata tcaaaatagc agattttggt ttcagtaacc tcttcactcc
     2941 tgggcagctg ctgaagacct ggtgtggcag ccctccctat gctgcacctg aactctttga
     3001 aggaaaagaa tatgatgggc ccaaagtgga catctggagc cttggagttg tcctctacgt
     3061 gcttgtgtgc ggtgccctgc catttgatgg aagcacactg cagaatctgc gggcccgcgt
     3121 gctgagtgga aagttccgca tcccattttt tatgtccaca gaatgtgagc atttgatccg
     3181 ccatatgttg gtgttagatc ccaataagcg cctctccatg gagcagatct gcaagcacaa
     3241 gtggatgaag ctaggggacg ccgatcccaa ctttgacagg ttaatagctg aatgccaaca
     3301 actaaaggaa gaaagacagg tggaccccct gaatgaggat gtcctcttgg ccatggagga
     3361 catgggactg gacaaagaac agacactgca gtcattaaga tcagatgcct atgatcacta
     3421 tagtgcaatc tacagcctgc tgtgtgatcg acataagaga cataaaaccc tgcgtctcgg
     3481 agcacttcct agcatgcccc gagccctggc ctttcaagca ccagtcaata tccaggcgga
     3541 gcaggcaggt actgctatga acatcagcgt tccccaggtg cagctgatca acccagagaa
     3601 ccaaattgtg gagccggatg ggacactgaa tttggacagt gatgagggtg aagagccttc
     3661 ccctgaagca ttggtgcgct atttgtcaat gaggaggcac acagtgggtg tggctgaccc
     3721 acgcacggaa gttatggaag atctgcagaa gctcctacct ggctttcctg gagtcaaccc
     3781 ccaggctcca ttcctgcagg tggcccctaa tgtgaacttc atgcacaacc tgttgcctat
     3841 gcaaaacttg caaccaaccg ggcaacttga gtacaaggag cagtctctcc tacagccgcc
     3901 cacgctacag ctgttgaatg gaatgggccc ccttggccgg agggcatcag atggaggagc
     3961 caacatccaa ctgcatgccc agcagctgct gaagcgccca cggggaccct ctccgcttgt
     4021 caccatgaca ccagcagtgc cagcagttac ccctgtggac gaggagagct cagacgggga
     4081 gccagaccag gaagctgtgc agagctctac ctacaaggac tccaacactc tgcacctccc
     4141 tacggagcgt ttctcccctg tgcgccggtt ctcagatggg gctgcgagca tccaggcctt
     4201 caaagctcac ctggaaaaaa tgggcaacaa cagcagcatc aaacagctgc agcaggagtg
     4261 tgagcagctg cagaagatgt acggggggca gattgatgaa agaaccctgg agaagaccca
     4321 gcagcagcat atgttatacc agcaggagca gcaccatcaa attctccagc aacaaattca
     4381 agactctatc tgtcctcctc agccatctcc acctcttcag gctgcatgtg aaaatcagcc
     4441 agccctcctt acccatcagc tccagaggtt aaggattcag ccttcaagcc caccccccaa
     4501 ccaccccaac aaccatctct tcaggcagcc cagtaatagt cctcccccca tgagcagtgc
     4561 catgatccag cctcacgggg ctgcatcttc ttcccagttt caaggcttac cttcccgcag
     4621 tgcaatcttt cagcagcaac ctgagaactg ttcctctcct cccaacgtgg cactaacctg
     4681 cttgggtatg cagcagcctg ctcagtcaca gcaggtcacc atccaagtcc aagagcctgt
     4741 tgacatgctc agcaacatgc caggcacagc tgcaggctcc agtgggcgcg gcatctccat
     4801 cagccccagt gctggtcaga tgcagatgca gcaccgtacc aacctgatgg ccaccctcag
     4861 ctatgggcac cgtcccttgt ccaagcagct gagtgctgac agtgcagagg ctcacagctt
     4921 gaacgtgaat cggttctccc ctgctaacta cgaccaggcg catttacacc cccatctgtt
     4981 ttcggaccag tcccggggtt cccccagcag ctacagccct tcaacaggag tggggttctc
     5041 tccaacccaa gccctgaaag tccctccact tgaccaattc cccaccttcc ctcccagtgc
     5101 acatcagcag ccgccacact ataccacgtc ggcactacag caggccctgc tgtctcccac
     5161 gccgccagac tatacaagac accagcaggt accccacatc cttcaaggac tgctttctcc
     5221 ccggcattcg ctcaccggcc actcggacat ccggctgccc ccaacagagt ttgcacagct
     5281 cattaaaagg cagcagcaac aacggcagca gcagcagcaa cagcagcaac agcaagaata
     5341 ccaggaactg ttcaggcaca tgaaccaagg ggatgcgggg agtctggctc ccagccttgg
     5401 gggacagagc atgacagagc gccaggcttt atcttatcaa aatgctgact cttatcacca
     5461 tcacaccagc ccccagcatc tgctacaaat cagggcacaa gaatgtgtct cacaggcttc
     5521 ctcacccacc ccgccccacg ggtatgctca ccagccggca ctgatgcatt cagagagcat
     5581 ggaggaggac tgctcgtgtg agggggccaa ggatggcttc caagacagta agagttcaag
     5641 tacattgacc aaaggttgcc atgacagccc tctgctcttg agtaccggtg gacctgggga
     5701 ccctgaatct ttgctaggaa ctgtgagtca tgcccaagaa ttggggatac atccctatgg
     5761 tcatcagcca actgctgcat tcagtaaaaa taaggtgccc agcagagagc ctgtcatagg
     5821 gaactgcatg gatagaagtt ctccaggaca agcagtggag ctgccggatc acaatgggct
     5881 cgggtaccca gcacgcccct ccgtccatga gcaccacagg ccccgggccc tccagagaca
     5941 ccacacgatc cagaacagcg acgatgctta tgtacagctg gataacttgc caggaatgag
     6001 tctcgtggct gggaaagcac ttagctctgc ccggatgtcg gatgcagttc tcagtcagtc
     6061 ttcgctcatg ggcagccagc agtttcagga tggggaaaat gaggaatgtg gggcaagcct
     6121 gggaggtcat gagcacccag acctgagtga tggcagccag catttaaact cctcttgcta
     6181 tccatctacg tgtattacag acattctgct cagctacaag caccccgaag tctccttcag
     6241 catggagcag gcaggcgtgg gatccgaaca aaaactaata tcagaggaag atttggaaca
     6301 gaaactaatc agtgaagaag atttagagca aaagttaatt tccgaggagg acttatagcg
     6361 gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     6421 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg
     6481 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     6541 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     6601 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac
     6661 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc
     6721 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc
     6781 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct
     6841 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg
     6901 accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga
     6961 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg
     7021 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct
     7081 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac
     7141 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa
     7201 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta
     7261 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag
     7321 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat
     7381 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta
     7441 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct
     7501 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga
     7561 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg
     7621 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg
     7681 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac
     7741 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     7801 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     7861 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     7921 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     7981 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     8041 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     8101 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     8161 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     8221 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     8281 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca
     8341 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     8401 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     8461 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     8521 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     8581 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     8641 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     8701 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     8761 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     8821 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct
     8881 tcgccctcag acgagtcgga tctccctttg gccgcctccc cgcctggtac ctttaagacc
     8941 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     9001 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg
     9061 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9121 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9181 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     9241 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     9301 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9361 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9421 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg
     9481 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9541 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca
     9601 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9661 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9721 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9781 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9841 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9901 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     9961 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10021 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10081 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10141 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10201 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10261 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10321 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10381 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10441 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10501 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10561 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10621 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10681 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10741 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10801 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10861 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10921 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10981 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11041 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11101 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11161 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11221 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11281 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11341 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11401 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11461 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11521 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11581 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11641 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    11701 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11761 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    11821 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
    11881 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
    11941 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
    12001 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    12061 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
    12121 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    12181 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
