LOCUS       V026076                 9523 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026076
VERSION     V026076
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4422
                     /label="SFPQ(NM_005066)"
                     /note="SFPQ(NM_005066)"
                     /gene="SFPQ"
     CDS             4423..4449
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4493..4704
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4717..4985
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5012..5611
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5618..6206
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6280..6513
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6585..6706
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6725..6860
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(6898..6914)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6922..6938
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6946..6976)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6991..7012
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7300..7888)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8059..8919)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8920..9024)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9498..9514
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtctcgg gatcggttcc ggagtcgtgg cggtggcggt
     2341 ggtggcttcc acaggcgtgg aggaggcggc ggccgcggcg gcctccacga cttccgttct
     2401 ccgccgcccg gcatgggcct caatcagaat cgcggcccca tgggtcctgg cccgggccag
     2461 agcggcccta agcctccgat cccgccaccg cctccacacc aacagcagca acagccacca
     2521 ccgcagcagc caccgccgca gcagccgcca ccgcatcagc cgccgccgca tccacagccg
     2581 catcagcagc agcagccgcc gccaccgccg caggactctt ccaagcccgt cgttgctcag
     2641 ggacccggcc ccgctcccgg agtaggcagc gcaccaccag cctccagctc ggccccgccc
     2701 gccactccac caacctcggg ggccccgcca gggtccgggc caggcccgac tccgaccccg
     2761 ccgcctgcag tcacctcggc ccctcccggg gcgccgccac ccaccccgcc aagcagcggg
     2821 gtccctacca cacctcctca ggccggaggc ccgccgcctc cgcccgcggc agtcccgggc
     2881 ccgggtccag ggcctaagca gggcccaggt ccgggtggtc ccaaaggcgg caaaatgcct
     2941 ggcgggccga agccaggtgg cggcccgggc ctaagtacgc ctggcggcca ccccaagccg
     3001 ccgcatcgag gcggcgggga gccccgcggg ggccgccagc accacccgcc ctaccaccag
     3061 cagcatcacc aggggccccc gcccggcggg cccggcggcc gcagcgagga gaagatctcg
     3121 gactcggagg ggtttaaagc caatttgtct ctcttgagga ggcctggaga gaaaacttac
     3181 acacagcgat gtcggttgtt tgttgggaat ctacctgctg atatcacgga ggatgaattc
     3241 aaaagactat ttgctaaata tggagaacca ggagaagttt ttatcaacaa aggcaaagga
     3301 ttcggattta ttaagcttga atctagagct ttggctgaaa ttgccaaagc cgaactggat
     3361 gatacaccca tgagaggtag acagcttcga gttcgctttg ccacacatgc tgctgccctt
     3421 tctgttcgta atctttcacc ttatgtttcc aatgaactgt tggaagaagc ctttagccaa
     3481 tttggtccta ttgaaagggc tgttgtaata gtggatgatc gtggaagatc tacagggaaa
     3541 ggcattgttg aatttgcttc taagccagca gcaagaaagg catttgaacg atgcagtgaa
     3601 ggtgttttct tactgacgac aactcctcgt ccagtcattg tggaaccact tgaacaacta
     3661 gatgatgaag atggtcttcc tgaaaaactt gcccagaaga atccaatgta tcaaaaggag
     3721 agagaaaccc ctcctcgttt tgcccagcat ggcacgtttg agtacgaata ttctcagcga
     3781 tggaagtctt tggatgaaat ggaaaaacag caaagggaac aagttgaaaa aaacatgaaa
     3841 gatgcaaaag acaaattgga aagtgaaatg gaagatgcct atcatgaaca tcaggcaaat
     3901 cttttgcgcc aagatctgat gagacgacag gaagaattaa gacgcatgga agaacttcac
     3961 aatcaagaaa tgcagaaacg taaagaaatg caattgaggc aagaggagga acgacgtaga
     4021 agagaggaag agatgatgat tcgtcaacgt gagatggaag aacaaatgag gcgccaaaga
     4081 gaggaaagtt acagccgaat gggctacatg gatccacggg aaagagacat gcgaatgggt
     4141 ggcggaggag caatgaacat gggagatccc tatggttcag gaggccagaa atttccacct
     4201 ctaggaggtg gtggtggcat aggttatgaa gctaatcctg gcgttccacc agcaaccatg
     4261 agtggttcca tgatgggaag tgacatgcgt actgagcgct ttgggcaggg aggtgcgggg
     4321 cctgtgggtg gacagggtcc tagaggaatg gggcctggaa ctccagcagg atatggtaga
     4381 gggagagaag agtacgaagg cccaaacaaa aaaccccgat tttacccata cgacgtacca
     4441 gattacgctt agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     4501 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     4561 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     4621 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     4681 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     4741 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     4801 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     4861 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     4921 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     4981 agatccaagc tgtgaccggc gcctacgcta gatgaccgag tacaagccca cggtgcgcct
     5041 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     5101 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     5161 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5221 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5281 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     5341 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     5401 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5461 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     5521 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5581 ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat caacctctgg attacaaaat
     5641 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5701 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5761 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5821 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5881 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5941 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6001 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6061 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6121 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6181 gatctccctt tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag
     6241 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6301 aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     6361 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6421 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6481 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     6541 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     6601 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6661 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     6721 agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6781 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6841 aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc
     6901 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6961 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7021 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7081 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7141 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7201 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7261 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7321 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7381 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7441 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7501 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7561 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7621 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7681 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7741 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7801 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7861 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7921 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7981 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8041 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8101 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8161 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8221 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8281 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8341 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8401 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8461 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8521 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8581 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8641 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8701 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8761 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8821 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8881 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8941 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9001 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     9061 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     9121 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     9181 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     9241 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     9301 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     9361 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     9421 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     9481 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
