LOCUS       V034666                10316 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034666
VERSION     V034666
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4390
                     /label="SEC14L5(NM_014692)"
                     /note="SEC14L5(NM_014692)"
                     /gene="SEC14L5"
     CDS             4405..4470
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4514..4725
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4738..5006
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5041..5748
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5758..5811
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5812..6411
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6412..6999
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7073..7306
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7378..7499
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7518..7653
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7691..7707)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7715..7731
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7739..7769)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7784..7805
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8093..8681)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8852..9712)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9713..9817)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10291..10307
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggtgca aagataccag tctcctgtcc gagtctacaa
     2341 gtacccgttt gagctggtca tggcggccta cgagaagcgt ttccccacgt gcccacagat
     2401 cccagtcttc ctgggcagcg aggtcttgcg cgagtcccgc agcccggacg gggctgtgca
     2461 cgtggtggag cggagctgcc ggctgcgcgt ggacgccccg cggctgctgc ggaagatcgc
     2521 aggtgttgag cacgtggtct tcgtgcagac aaacatcttg aactggaagg agaggacgct
     2581 cctcatcgaa gcgcacaatg agaccttcgc caaccgcgtg gtggtgaacg agcactgcag
     2641 ctacacggtc caccctgaga atgaagactg gacttgcttc gagcagtctg cctcactgga
     2701 cattcggtct ttctttggct ttgaaaatgc cttggagaag atcgccatga agcagtacac
     2761 cgccaacgtc aagaggggga aggaggtgat tgagcattac ctgaatgagc tcatctccca
     2821 gggtacctcg cacattccgc gctggacgcc tgccccagtc cgtgaggagg atgcccgcaa
     2881 ccaggctgga ccgagggacc ccagctccct ggaggcccac gggccccgta gcaccctggg
     2941 gcccgctctg gaggcggtca gtatggacgg ggacaagctg gatgcggact acattgagag
     3001 gtgcctgggc cacctcacgc ccatgcagga gagctgcctg atccagcttc ggcactggtt
     3061 acaggagacc cacaaaggca agattcccaa agatgagcac atccttcggt tcctgcgggc
     3121 tcatgacttc cacctggaca aggcccggga aatgctgcgc cagtccttga gctggcgcaa
     3181 gcagcaccag gtggatctcc tccttcagac ctggcaaccc cctgccctgc tggaggagtt
     3241 ctatgcaggg ggctggcatt accaggacat agatggccgc cccctctaca tcctccgcct
     3301 gggccagatg gacaccaaag gcttgatgaa ggccgtgggg gaggaggcgc tgctgcggca
     3361 tgttctctcc gtcaacgagg aaggacagaa gcggtgtgag gggagcacaa ggcagctggg
     3421 ccgtcccatc agctcctgga cctgcctgct agacctggag ggactcaaca tgcggcacct
     3481 gtggcggccg ggggtgaagg ccctgctgcg gatgattgag gtggttgagg acaattaccc
     3541 agagaccctg ggtcggctgc tcatcgtgcg agccccccga gtcttccccg tgctctggac
     3601 actgatcagc cccttcatca atgagaacac caggcggaag ttcctcatct acagtggcag
     3661 caactaccag ggacccggag gccttgtgga ctatctggat agagaagtga tccctgactt
     3721 ccttggggga gagagtgtgt gtaatgtccc cgaaggaggg ctggtcccca agtccctcta
     3781 catgacagaa gaggagcagg agcacacgga ccagctgtgg cagtggagtg agacctacca
     3841 ttcagccagc gtgctccgcg gagcccccca cgaggtggcc gtggagatcc tggaaggaga
     3901 gtcggtcatc acctgggact ttgacatcct gcgaggggac gtggtgttca gcctgtacca
     3961 caccaagcag gcgcccaggc tgggcgcccg ggaaccgggg accagggcca gcgggcagct
     4021 gatcgacaaa ggctgggtcc tgggcaggga ttacagccgt gtggaggctc cccttgtctg
     4081 ccgggagggg gagagcatcc agggctccca tgtgacccgg tggcccggcg tctacctgct
     4141 ccagtggcaa atgcacagcc cccccagcag cgtggcctgc agcctcccgg gtgtggacga
     4201 tgtcctgacg gctctgcaca gccccgggcc caagtgcaaa cttctctact actgtgaggt
     4261 gctcgcctct gaggacttca ggggctccat gtccagcctg gaatcctgca ccagcggctt
     4321 ctcccagctc agcgccgcca cctcgtcctc ctcctccggc cagtctcata gcagctccct
     4381 ggtctccaga ctcgagtcgg atccgactac aaagaccatg acggtgatta taaagatcat
     4441 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     4501 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     4561 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     4621 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     4681 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     4741 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     4801 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     4861 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     4921 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     4981 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     5041 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     5101 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     5161 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     5221 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     5281 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     5341 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     5401 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     5461 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     5521 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     5581 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     5641 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     5701 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg atctcgcgag
     5761 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
     5821 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     5881 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     5941 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6001 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6061 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     6121 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6181 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6241 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6301 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6361 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
     6421 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6481 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6541 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6601 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6661 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6721 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6781 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     6841 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6901 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc
     6961 cctcagacga gtcggatctc cctttggccg cctccccgcc tggtaccttt aagaccaatg
     7021 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7081 ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta
     7141 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7201 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7261 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7321 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     7381 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7441 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7501 catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7561 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7621 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt
     7681 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7741 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7801 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7861 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7921 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7981 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8041 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8101 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8161 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8221 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8281 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8341 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8401 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8461 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8521 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8581 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8641 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8701 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8761 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8821 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8881 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8941 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9001 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9061 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9121 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9181 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9241 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9301 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9361 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9421 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9481 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9541 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9601 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9661 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9721 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9781 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9841 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     9901 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     9961 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    10021 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    10081 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    10141 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    10201 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    10261 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
