LOCUS       V041640                10282 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041640
VERSION     V041640
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4308
                     /label="SDHA(NM_004168)"
                     /note="SDHA(NM_004168)"
                     /gene="SDHA"
     CDS             4315..4380
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4424..4635
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4648..4916
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4945..4954
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4975..5637
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5707..5760
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5761..6360
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6361..6949
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7023..7256
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7328..7449
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7489..7624
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7657..7673)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7681..7697
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7705..7735)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7750..7771
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8059..8647)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8818..9678)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9679..9783)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10257..10273
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cgggggtccg gggcctgtcg
     2341 cggctgctga gcgctcggcg cctggcgctg gccaaggcgt ggccaacagt gttgcaaaca
     2401 ggaacccgag gttttcactt cactgttgat gggaacaaga gggcatctgc taaagtttca
     2461 gattccattt ctgctcagta tccagtagtg gatcatgaat ttgatgcagt ggtggtaggc
     2521 gctggagggg caggcttgcg agctgcattt ggcctttctg aggcagggtt taatacagca
     2581 tgtgttacca agctgtttcc taccaggtca cacactgttg cagcacaggg aggaatcaat
     2641 gctgctctgg ggaacatgga ggaggacaac tggaggtggc atttctacga caccgtgaag
     2701 ggctccgact ggctggggga ccaggatgcc atccactaca tgacggagca ggcccccgcc
     2761 gccgtggtcg agctagaaaa ttatggcatg ccgtttagca gaactgaaga tgggaagatt
     2821 tatcagcgtg catttggtgg acagagcctc aagtttggaa agggcgggca ggcccatcgg
     2881 tgctgctgtg tggctgatcg gactggccac tcgctattgc acaccttata tggaaggtct
     2941 ctgcgatatg ataccagcta ttttgtggag tattttgcct tggatctcct gatggagaat
     3001 ggggagtgcc gtggtgtcat cgcactgtgc atagaggacg ggtccatcca tcgcataaga
     3061 gcaaagaaca ctgttgttgc cacaggaggc tacgggcgca cctacttcag ctgcacgtct
     3121 gcccacacca gcactggcga cggcacggcc atgatcacca gggcaggcct tccttgccag
     3181 gacctagagt ttgttcagtt ccaccctaca ggcatatatg gtgctggttg tctcattacg
     3241 gaaggatgtc gtggagaggg aggcattctc attaacagtc aaggcgaaag gtttatggag
     3301 cgatacgccc ctgtcgcgaa ggacctggcg tctagagatg tggtgtctcg gtccatgact
     3361 ctggagatcc gagaaggaag aggctgtggc cctgagaaag atcacgtcta cctgcagctg
     3421 caccacctac ctccagagca gctggccacg cgcctgcctg gcatttcaga gacagccatg
     3481 atcttcgctg gcgtggacgt cacgaaggag ccgatccctg tcctccccac cgtgcattat
     3541 aacatgggcg gcattcccac caactacaag gggcaggtcc tgaggcacgt gaatggccag
     3601 gatcagattg tgcccggcct gtacgcctgt ggggaggccg cctgtgcctc ggtacatggt
     3661 gccaaccgcc tcggggcaaa ctcgctcttg gacctggttg tctttggtcg ggcatgtgcc
     3721 ctgagcatcg aagagtcatg caggcctgga gataaagtcc ctccaattaa accaaacgct
     3781 ggggaagaat ctgtcatgaa tcttgacaaa ttgagatttg ctgatggaag cataagaaca
     3841 tcggaactgc gactcagcat gcagaagtca atgcaaaatc atgctgccgt gttccgtgtg
     3901 ggaagcgtgt tgcaagaagg ttgtgggaaa atcagcaagc tctatggaga cctaaagcac
     3961 ctgaagacgt tcgaccgggg aatggtctgg aacacggacc tggtggagac cctggagctg
     4021 cagaacctga tgctgtgtgc gctgcagacc atctacggag cagaggcacg gaaggagtca
     4081 cggggcgcgc atgccaggga agactacaag gtgcggattg atgagtacga ttactccaag
     4141 cccatccagg ggcaacagaa gaagcccttt gaggagcact ggaggaagca caccctgtcc
     4201 tatgtggacg ttggcactgg gaaggtcact ctggaatata gacccgtgat cgacaaaact
     4261 ttgaacgagg ctgactgtgc caccgtcccg ccagccattc gctcctacgg atccgactac
     4321 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     4381 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4441 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     4501 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4561 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4621 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     4681 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     4741 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     4801 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     4861 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     4921 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc
     4981 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     5041 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc
     5101 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     5161 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     5221 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
     5281 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag
     5341 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     5401 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc
     5461 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     5521 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac
     5581 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga
     5641 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga
     5701 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     5761 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     5821 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     5881 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5941 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6001 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6061 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6121 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6181 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6241 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6301 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6361 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6421 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6481 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6541 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6601 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6661 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6721 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6781 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6841 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6901 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt
     6961 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7021 actggaaggg ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc
     7081 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7141 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7201 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7261 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7321 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7381 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7441 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     7501 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7561 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7621 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     7681 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7741 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7801 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7861 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7921 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7981 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8461 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8641 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8701 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8761 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8821 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8881 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8941 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9001 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9061 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9121 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9181 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9241 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9301 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9361 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9421 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9481 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9541 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9601 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9661 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9721 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9781 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     9841 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     9901 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     9961 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    10021 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    10081 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    10141 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    10201 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    10261 aacgacggcc agtgccaagc tg
//
