LOCUS       V039641                11199 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039641
VERSION     V039641
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5281
                     /label="SCUBE3(NM_152753)"
                     /note="SCUBE3(NM_152753)"
                     /gene="SCUBE3"
     CDS             5288..5353
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5397..5608
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5621..5889
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5924..6631
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6641..6694
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6695..7294
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7295..7882
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7956..8189
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8261..8382
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8401..8536
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8574..8590)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8598..8614
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8622..8652)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8667..8688
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8976..9564)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9735..10595)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10596..10700)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11174..11190
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgggctc ggggcgcgta cccgggctct gcctgcttgt
     2341 cctgctggtc cacgcccgcg ccgcccagta cagcaaagcc gcgcaagatg tggatgagtg
     2401 tgtggagggg actgacaact gccacatcga tgctatctgc cagaacaccc cgaggtcata
     2461 caagtgcatc tgcaagtctg gctacacagg ggacggcaaa cactgcaaag acgtggatga
     2521 gtgcgagcga gaggataatg caggttgtgt gcatgactgt gtcaacatcc ctggcaatta
     2581 ccggtgtacc tgctatgatg gattccacct ggcacatgac ggacacaact gtctggatgt
     2641 ggacgagtgt gccgagggca acggcggctg tcagcagagc tgtgtcaaca tgatgggcag
     2701 ctatgagtgc cactgccggg aaggcttctt cctcagcgac aaccagcata cctgtatcca
     2761 gcggccagaa gaaggaatga attgcatgaa caagaaccac ggctgtgccc acatttgccg
     2821 ggagacaccc aaggggggta ttgcctgtga atgccgtcct ggctttgagc ttaccaagaa
     2881 ccaacgggac tgtaaattga catgcaacta tggtaacggc ggctgccagc acacgtgtga
     2941 tgacacagag cagggtcccc ggtgcggctg ccatatcaag tttgtgctcc ataccgacgg
     3001 gaagacatgc atcgagacct gtgctgtcaa caacgggggc tgtgacagta agtgccatga
     3061 tgcagcgact ggtgtccact gcacctgccc tgtgggcttc atgctgcagc cagacaggaa
     3121 gacgtgcaaa gatatagatg agtgccgctt aaacaacggg ggctgtgacc atatttgccg
     3181 caacacagtg ggcagcttcg aatgcagttg caagaaaggc tataagcttc tcatcaatga
     3241 gaggaactgc caggatatag acgagtgttc ctttgatcga acctgtgacc acatatgtgt
     3301 caacacacca ggaagcttcc agtgtctctg ccatcgtggc tacctgttgt atggtatcac
     3361 ccactgtggg gatgtggatg aatgcagcat caaccgggga ggttgccgct ttggctgcat
     3421 caacactcct ggcagctacc agtgtacctg cccagcaggc cagggtcggc tgcactggaa
     3481 tggcaaagat tgcacagagc cactgaagtg tcagggcagt cctggggcct cgaaagccat
     3541 gctcagctgc aaccggtctg gcaagaagga cacctgtgcc ctgacctgtc cctccagggc
     3601 ccgatttttg ccagagtctg agaatggctt cacggtgagc tgtgggaccc ccagccccag
     3661 ggctgctcca gcccgagctg gccacaatgg gaacagcacc aactccaacc actgccatga
     3721 ggctgcagtg ctgtccatta aacaacgggc ctccttcaag atcaaggatg ccaaatgccg
     3781 tttgcacctg cgaaacaaag gcaaaacaga ggaggctggc agaatcacag ggccaggtgg
     3841 tgccccctgc tctgaatgcc aggtcacctt catccacctt aagtgtgact cctctcggaa
     3901 gggcaagggc cgacgggccc ggacccctcc aggcaaagag gtcacaaggc tcaccctgga
     3961 actggaggca gaggtcagag ccgaagaaac cacagccagc tgtgggctgc cctgcctccg
     4021 acagcgaatg gaacggcggc tgaaaggatc cctgaagatg ctcagaaagt ccatcaacca
     4081 ggaccgcttc ctgctgcgcc tggcaggcct tgattatgag ctggcccaca agccgggcct
     4141 ggtagccggg gagcgagcag agccgatgga gtcctgtagg cccgggcagc accgtgctgg
     4201 gaccaagtgt gtcagctgcc cgcagggaac gtattaccac ggccagacgg agcagtgtgt
     4261 gccatgccca gcgggcacct tccaggagag agaagggcag ctctcctgcg acctttgccc
     4321 tgggagtgat gcccacgggc ctcttggagc caccaacgtc accacgtgtg caggtcagtg
     4381 cccacctggc caacactctg tagatgggtt caagccctgt cagccatgcc cacgtggcac
     4441 ctaccaacct gaagcaggac ggaccctatg cttcccttgt ggtgggggcc tcaccaccaa
     4501 gcatgaaggg gccatttcct tccaagactg tgacaccaaa gtccagtgct ccccagggca
     4561 ctactacaac accagcatcc accgctgtat tcgctgtgcc atgggctcct atcagcccga
     4621 cttccgtcag aacttctgca gccgctgtcc aggaaacaca agcacagact ttgatggctc
     4681 taccagtgtg gcccaatgca agaatcgtca gtgtggtggg gagctgggtg agttcactgg
     4741 ctatattgag tcccccaact acccgggcaa ctacccagct ggtgtggagt gcatctggaa
     4801 catcaacccc ccacccaagc gcaagatcct tatcgtggta ccagagatct tcctgccatc
     4861 tgaggatgag tgtggggacg tcctcgtcat gagaaagaac tcatccccat cctccattac
     4921 cacttatgag acctgccaga cctacgagcg tcccattgcc ttcactgccc gttccaggaa
     4981 gctctggatc aacttcaaga caagcgaggc caacagcgcc cgtggcttcc agattcccta
     5041 tgttacctat gatgaggact atgagcagct ggtagaagac attgtgcgag atggccggct
     5101 ctatgcctct gaaaaccacc aggagatttt aaaggacaag aagctcatca aggccttctt
     5161 tgaggtgcta gcccaccccc agaactactt caagtacaca gagaaacaca aggagatgct
     5221 gccaaaatcc ttcatcaagc tgctccgctc caaagtttcc agcttcctga ggccctacaa
     5281 aggatccgac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga
     5341 tgacgatgac aagtagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     5401 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     5461 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     5521 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5581 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     5641 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     5701 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     5761 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     5821 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     5881 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggtga gcaagggcga
     5941 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     6001 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     6061 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     6121 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     6181 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     6241 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     6301 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     6361 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     6421 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     6481 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     6541 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     6601 ctccaccggc ggcatggacg agctgtacaa gggatctcgc gagggcagag gaagtcttct
     6661 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg
     6721 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     6781 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     6841 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6901 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6961 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7021 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     7081 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     7141 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     7201 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     7261 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg
     7321 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7381 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7441 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7501 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7561 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7621 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7681 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     7741 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7801 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat
     7861 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     7921 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7981 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     8041 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8101 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8161 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     8221 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     8281 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8341 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     8401 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8461 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8521 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag
     8581 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8641 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8701 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8761 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8821 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8881 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8941 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9001 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9061 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9121 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9181 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9241 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9301 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9361 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     9421 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9481 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9541 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9601 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9661 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9721 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9781 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9841 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9901 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9961 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10021 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10081 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10141 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10201 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10261 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10321 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10381 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10441 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10501 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10561 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10621 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10681 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    10741 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    10801 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    10861 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    10921 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    10981 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    11041 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    11101 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    11161 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
