LOCUS V026585 14338 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026585 VERSION V026585 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..8364 /label="SCN5A(NM_198056)" /note="SCN5A(NM_198056)" /gene="SCN5A" CDS 8371..8436 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 8480..8691 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 8704..8972 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 9001..9010 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 9031..9693 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 9763..9816 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 9817..10416 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 10417..11005 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 11079..11312 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 11384..11505 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 11545..11680 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(11713..11729) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 11737..11753 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(11761..11791) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 11806..11827 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(12115..12703) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(12874..13734) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(13735..13839) /gene="bla" /label="AmpR promoter" primer_bind 14313..14329 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg caaacttcct attacctcgg 2341 ggcaccagca gcttccgcag gttcacacgg gagtccctgg cagccatcga gaagcgcatg 2401 gcagagaagc aagcccgcgg ctcaaccacc ttgcaggaga gccgagaggg gctgcccgag 2461 gaggaggctc cccggcccca gctggacctg caggcctcca aaaagctgcc agatctctat 2521 ggcaatccac cccaagagct catcggagag cccctggagg acctggaccc cttctatagc 2581 acccaaaaga ctttcatcgt actgaataaa ggcaagacca tcttccggtt cagtgccacc 2641 aacgccttgt atgtcctcag tcccttccac cccatccgga gagcggctgt gaagattctg 2701 gttcactcgc tcttcaacat gctcatcatg tgcaccatcc tcaccaactg cgtgttcatg 2761 gcccagcacg accctccacc ctggaccaag tatgtcgagt acaccttcac cgccatttac 2821 acctttgagt ctctggtcaa gattctggct cgaggcttct gcctgcacgc gttcactttc 2881 cttcgggacc catggaactg gctggacttt agtgtgatta tcatggcata cacaactgaa 2941 tttgtggacc tgggcaatgt ctcagcctta cgcaccttcc gagtcctccg ggccctgaaa 3001 actatatcag tcatttcagg gctgaagacc atcgtggggg ccctgatcca gtctgtgaag 3061 aagctggctg atgtgatggt cctcacagtc ttctgcctca gcgtctttgc cctcatcggc 3121 ctgcagctct tcatgggcaa cctaaggcac aagtgcgtgc gcaacttcac agcgctcaac 3181 ggcaccaacg gctccgtgga ggccgacggc ttggtctggg aatccctgga cctttacctc 3241 agtgatccag aaaattacct gctcaagaac ggcacctctg atgtgttact gtgtgggaac 3301 agctctgacg ctgggacatg tccggagggc taccggtgcc taaaggcagg cgagaacccc 3361 gaccacggct acaccagctt cgattccttt gcctgggcct ttcttgcact cttccgcctg 3421 atgacgcagg actgctggga gcgcctctat cagcagaccc tcaggtccgc agggaagatc 3481 tacatgatct tcttcatgct tgtcatcttc ctggggtcct tctacctggt gaacctgatc 3541 ctggccgtgg tcgcaatggc ctatgaggag caaaaccaag ccaccatcgc tgagaccgag 3601 gagaaggaaa agcgcttcca ggaggccatg gaaatgctca agaaagaaca cgaggccctc 3661 accatcaggg gtgtggatac cgtgtcccgt agctccttgg agatgtcccc tttggcccca 3721 gtaaacagcc atgagagaag aagcaagagg agaaaacgga tgtcttcagg aactgaggag 3781 tgtggggagg acaggctccc caagtctgac tcagaagatg gtcccagagc aatgaatcat 3841 ctcagcctca cccgtggcct cagcaggact tctatgaagc cacgttccag ccgcgggagc 3901 attttcacct ttcgcaggcg agacctgggt tctgaagcag attttgcaga tgatgaaaac 3961 agcacagcgg gggagagcga gagccaccac acatcactgc tggtgccctg gcccctgcgc 4021 cggaccagtg cccagggaca gcccagtccc ggaacctcgg ctcctggcca cgccctccat 4081 ggcaaaaaga acagcactgt ggactgcaat ggggtggtct cattactggg ggcaggcgac 4141 ccagaggcca catccccagg aagccacctc ctccgccctg tgatgctaga gcacccgcca 4201 gacacgacca cgccatcgga ggagccaggc gggccccaga tgctgacctc ccaggctccg 4261 tgtgtagatg gcttcgagga gccaggagca cggcagcggg ccctcagcgc agtcagcgtc 4321 ctcaccagcg cactggaaga gttagaggag tctcgccaca agtgtccacc atgctggaac 4381 cgtctcgccc agcgctacct gatctgggag tgctgcccgc tgtggatgtc catcaagcag 4441 ggagtgaagt tggtggtcat ggacccgttt actgacctca ccatcactat gtgcatcgta 4501 ctcaacacac tcttcatggc gctggagcac tacaacatga caagtgaatt cgaggagatg 4561 ctgcaggtcg gaaacctggt cttcacaggg attttcacag cagagatgac cttcaagatc 4621 attgccctcg acccctacta ctacttccaa cagggctgga acatcttcga cagcatcatc 4681 gtcatcctta gcctcatgga gctgggcctg tcccgcatga gcaacttgtc ggtgctgcgc 4741 tccttccgcc tgctgcgggt cttcaagctg gccaaatcat ggcccaccct gaacacactc 4801 atcaagatca tcgggaactc agtgggggca ctggggaacc tgacactggt gctagccatc 4861 atcgtgttca tctttgctgt ggtgggcatg cagctctttg gcaagaacta ctcggagctg 4921 agggacagcg actcaggcct gctgcctcgc tggcacatga tggacttctt tcatgccttc 4981 ctcatcatct tccgcatcct ctgtggagag tggatcgaga ccatgtggga ctgcatggag 5041 gtgtcggggc agtcattatg cctgctggtc ttcttgcttg ttatggtcat tggcaacctt 5101 gtggtcctga atctcttcct ggccttgctg ctcagctcct tcagtgcaga caacctcaca 5161 gcccctgatg aggacagaga gatgaacaac ctccagctgg ccctggcccg catccagagg 5221 ggcctgcgct ttgtcaagcg gaccacctgg gatttctgct gtggtctcct gcggcagcgg 5281 cctcagaagc ccgcagccct tgccgcccag ggccagctgc ccagctgcat tgccaccccc 5341 tactccccgc cacccccaga gacggagaag gtgcctccca cccgcaagga aacacggttt 5401 gaggaaggcg agcaaccagg ccagggcacc cccggggatc cagagcccgt gtgtgtgccc 5461 atcgctgtgg ccgagtcaga cacagatgac caagaagaag atgaggagaa cagcctgggc 5521 acggaggagg agtccagcaa gcagcaggaa tcccagcctg tgtccggtgg cccagaggcc 5581 cctccggatt ccaggacctg gagccaggtg tcagcgactg cctcctctga ggccgaggcc 5641 agtgcatctc aggccgactg gcggcagcag tggaaagcgg aaccccaggc cccagggtgc 5701 ggtgagaccc cagaggacag ttgctccgag ggcagcacag cagacatgac caacaccgct 5761 gagctcctgg agcagatccc tgacctcggc caggatgtca aggacccaga ggactgcttc 5821 actgaaggct gtgtccggcg ctgtccctgc tgtgcggtgg acaccacaca ggccccaggg 5881 aaggtctggt ggcggttgcg caagacctgc taccacatcg tggagcacag ctggttcgag 5941 acattcatca tcttcatgat cctactcagc agtggagcgc tggccttcga ggacatctac 6001 ctagaggagc ggaagaccat caaggttctg cttgagtatg ccgacaagat gttcacatat 6061 gtcttcgtgc tggagatgct gctcaagtgg gtggcctacg gcttcaagaa gtacttcacc 6121 aatgcctggt gctggctcga cttcctcatc gtagacgtct ctctggtcag cctggtggcc 6181 aacaccctgg gctttgccga gatgggcccc atcaagtcac tgcggacgct gcgtgcactc 6241 cgtcctctga gagctctgtc acgatttgag ggcatgaggg tggtggtcaa tgccctggtg 6301 ggcgccatcc cgtccatcat gaacgtcctc ctcgtctgcc tcatcttctg gctcatcttc 6361 agcatcatgg gcgtgaacct ctttgcgggg aagtttggga ggtgcatcaa ccagacagag 6421 ggagacttgc ctttgaacta caccatcgtg aacaacaaga gccagtgtga gtccttgaac 6481 ttgaccggag aattgtactg gaccaaggtg aaagtcaact ttgacaacgt gggggccggg 6541 tacctggccc ttctgcaggt ggcaacattt aaaggctgga tggacattat gtatgcagct 6601 gtggactcca gggggtatga agagcagcct cagtgggaat acaacctcta catgtacatc 6661 tattttgtca ttttcatcat ctttgggtct ttcttcaccc tgaacctctt tattggtgtc 6721 atcattgaca acttcaacca acagaagaaa aagttagggg gccaggacat cttcatgaca 6781 gaggagcaga agaagtacta caatgccatg aagaagctgg gctccaagaa gccccagaag 6841 cccatcccac ggcccctgaa caagtaccag ggcttcatat tcgacattgt gaccaagcag 6901 gcctttgacg tcaccatcat gtttctgatc tgcttgaata tggtgaccat gatggtggag 6961 acagatgacc aaagtcctga gaaaatcaac atcttggcca agatcaacct gctctttgtg 7021 gccatcttca caggcgagtg tattgtcaag ctggctgccc tgcgccacta ctacttcacc 7081 aacagctgga atatcttcga cttcgtggtt gtcatcctct ccatcgtggg cactgtgctc 7141 tcggacatca tccagaagta cttcttctcc ccgacgctct tccgagtcat ccgcctggcc 7201 cgaataggcc gcatcctcag actgatccga ggggccaagg ggatccgcac gctgctcttt 7261 gccctcatga tgtccctgcc tgccctcttc aacatcgggc tgctgctctt cctcgtcatg 7321 ttcatctact ccatctttgg catggccaac ttcgcttatg tcaagtggga ggctggcatc 7381 gacgacatgt tcaacttcca gaccttcgcc aacagcatgc tgtgcctctt ccagatcacc 7441 acgtcggccg gctgggatgg cctcctcagc cccatcctca acactgggcc gccctactgc 7501 gaccccactc tgcccaacag caatggctct cggggggact gcgggagccc agccgtgggc 7561 atcctcttct tcaccaccta catcatcatc tccttcctca tcgtggtcaa catgtacatt 7621 gccatcatcc tggagaactt cagcgtggcc acggaggaga gcaccgagcc cctgagtgag 7681 gacgacttcg atatgttcta tgagatctgg gagaaatttg acccagaggc cactcagttt 7741 attgagtatt cggtcctgtc tgactttgcc gatgccctgt ctgagccact ccgtatcgcc 7801 aagcccaacc agataagcct catcaacatg gacctgccca tggtgagtgg ggaccgcatc 7861 cattgcatgg acattctctt tgccttcacc aaaagggtcc tgggggagtc tggggagatg 7921 gacgccctga agatccagat ggaggagaag ttcatggcag ccaacccatc caagatctcc 7981 tacgagccca tcaccaccac actccggcgc aagcacgaag aggtgtcggc catggttatc 8041 cagagagcct tccgcaggca cctgctgcaa cgctctttga agcatgcctc cttcctcttc 8101 cgtcagcagg cgggcagcgg cctctccgaa gaggatgccc ctgagcgaga gggcctcatc 8161 gcctacgtga tgagtgagaa cttctcccga ccccttggcc caccctccag ctcctccatc 8221 tcctccactt ccttcccacc ctcctatgac agtgtcacta gagccaccag cgataacctc 8281 caggtgcggg ggtctgacta cagccacagt gaagatctcg ccgacttccc cccttctccg 8341 gacagggacc gtgagtccat cgtgggatcc gactacaaag accatgacgg tgattataaa 8401 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat 8461 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 8521 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 8581 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 8641 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 8701 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 8761 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 8821 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 8881 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 8941 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac 9001 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc 9061 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag 9121 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg 9181 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag 9241 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac 9301 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc 9361 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag 9421 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg 9481 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac 9541 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg 9601 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac 9661 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct 9721 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt 9781 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg 9841 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 9901 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 9961 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 10021 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 10081 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 10141 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 10201 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 10261 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 10321 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 10381 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt 10441 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 10501 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 10561 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 10621 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 10681 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 10741 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 10801 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg 10861 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 10921 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 10981 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 11041 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 11101 acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 11161 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 11221 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 11281 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 11341 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 11401 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 11461 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 11521 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 11581 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 11641 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa 11701 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 11761 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 11821 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 11881 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 11941 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 12001 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 12061 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 12121 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 12181 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 12241 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 12301 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 12361 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 12421 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 12481 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 12541 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 12601 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 12661 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 12721 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 12781 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 12841 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 12901 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 12961 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 13021 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 13081 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 13141 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 13201 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 13261 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 13321 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 13381 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 13441 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 13501 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 13561 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 13621 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 13681 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 13741 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 13801 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 13861 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 13921 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 13981 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 14041 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 14101 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 14161 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 14221 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 14281 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg //