LOCUS       V026585                14338 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026585
VERSION     V026585
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..8364
                     /label="SCN5A(NM_198056)"
                     /note="SCN5A(NM_198056)"
                     /gene="SCN5A"
     CDS             8371..8436
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8480..8691
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8704..8972
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      9001..9010
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             9031..9693
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             9763..9816
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9817..10416
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10417..11005
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11079..11312
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11384..11505
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11545..11680
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11713..11729)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11737..11753
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11761..11791)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11806..11827
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12115..12703)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12874..13734)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13735..13839)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14313..14329
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg caaacttcct attacctcgg
     2341 ggcaccagca gcttccgcag gttcacacgg gagtccctgg cagccatcga gaagcgcatg
     2401 gcagagaagc aagcccgcgg ctcaaccacc ttgcaggaga gccgagaggg gctgcccgag
     2461 gaggaggctc cccggcccca gctggacctg caggcctcca aaaagctgcc agatctctat
     2521 ggcaatccac cccaagagct catcggagag cccctggagg acctggaccc cttctatagc
     2581 acccaaaaga ctttcatcgt actgaataaa ggcaagacca tcttccggtt cagtgccacc
     2641 aacgccttgt atgtcctcag tcccttccac cccatccgga gagcggctgt gaagattctg
     2701 gttcactcgc tcttcaacat gctcatcatg tgcaccatcc tcaccaactg cgtgttcatg
     2761 gcccagcacg accctccacc ctggaccaag tatgtcgagt acaccttcac cgccatttac
     2821 acctttgagt ctctggtcaa gattctggct cgaggcttct gcctgcacgc gttcactttc
     2881 cttcgggacc catggaactg gctggacttt agtgtgatta tcatggcata cacaactgaa
     2941 tttgtggacc tgggcaatgt ctcagcctta cgcaccttcc gagtcctccg ggccctgaaa
     3001 actatatcag tcatttcagg gctgaagacc atcgtggggg ccctgatcca gtctgtgaag
     3061 aagctggctg atgtgatggt cctcacagtc ttctgcctca gcgtctttgc cctcatcggc
     3121 ctgcagctct tcatgggcaa cctaaggcac aagtgcgtgc gcaacttcac agcgctcaac
     3181 ggcaccaacg gctccgtgga ggccgacggc ttggtctggg aatccctgga cctttacctc
     3241 agtgatccag aaaattacct gctcaagaac ggcacctctg atgtgttact gtgtgggaac
     3301 agctctgacg ctgggacatg tccggagggc taccggtgcc taaaggcagg cgagaacccc
     3361 gaccacggct acaccagctt cgattccttt gcctgggcct ttcttgcact cttccgcctg
     3421 atgacgcagg actgctggga gcgcctctat cagcagaccc tcaggtccgc agggaagatc
     3481 tacatgatct tcttcatgct tgtcatcttc ctggggtcct tctacctggt gaacctgatc
     3541 ctggccgtgg tcgcaatggc ctatgaggag caaaaccaag ccaccatcgc tgagaccgag
     3601 gagaaggaaa agcgcttcca ggaggccatg gaaatgctca agaaagaaca cgaggccctc
     3661 accatcaggg gtgtggatac cgtgtcccgt agctccttgg agatgtcccc tttggcccca
     3721 gtaaacagcc atgagagaag aagcaagagg agaaaacgga tgtcttcagg aactgaggag
     3781 tgtggggagg acaggctccc caagtctgac tcagaagatg gtcccagagc aatgaatcat
     3841 ctcagcctca cccgtggcct cagcaggact tctatgaagc cacgttccag ccgcgggagc
     3901 attttcacct ttcgcaggcg agacctgggt tctgaagcag attttgcaga tgatgaaaac
     3961 agcacagcgg gggagagcga gagccaccac acatcactgc tggtgccctg gcccctgcgc
     4021 cggaccagtg cccagggaca gcccagtccc ggaacctcgg ctcctggcca cgccctccat
     4081 ggcaaaaaga acagcactgt ggactgcaat ggggtggtct cattactggg ggcaggcgac
     4141 ccagaggcca catccccagg aagccacctc ctccgccctg tgatgctaga gcacccgcca
     4201 gacacgacca cgccatcgga ggagccaggc gggccccaga tgctgacctc ccaggctccg
     4261 tgtgtagatg gcttcgagga gccaggagca cggcagcggg ccctcagcgc agtcagcgtc
     4321 ctcaccagcg cactggaaga gttagaggag tctcgccaca agtgtccacc atgctggaac
     4381 cgtctcgccc agcgctacct gatctgggag tgctgcccgc tgtggatgtc catcaagcag
     4441 ggagtgaagt tggtggtcat ggacccgttt actgacctca ccatcactat gtgcatcgta
     4501 ctcaacacac tcttcatggc gctggagcac tacaacatga caagtgaatt cgaggagatg
     4561 ctgcaggtcg gaaacctggt cttcacaggg attttcacag cagagatgac cttcaagatc
     4621 attgccctcg acccctacta ctacttccaa cagggctgga acatcttcga cagcatcatc
     4681 gtcatcctta gcctcatgga gctgggcctg tcccgcatga gcaacttgtc ggtgctgcgc
     4741 tccttccgcc tgctgcgggt cttcaagctg gccaaatcat ggcccaccct gaacacactc
     4801 atcaagatca tcgggaactc agtgggggca ctggggaacc tgacactggt gctagccatc
     4861 atcgtgttca tctttgctgt ggtgggcatg cagctctttg gcaagaacta ctcggagctg
     4921 agggacagcg actcaggcct gctgcctcgc tggcacatga tggacttctt tcatgccttc
     4981 ctcatcatct tccgcatcct ctgtggagag tggatcgaga ccatgtggga ctgcatggag
     5041 gtgtcggggc agtcattatg cctgctggtc ttcttgcttg ttatggtcat tggcaacctt
     5101 gtggtcctga atctcttcct ggccttgctg ctcagctcct tcagtgcaga caacctcaca
     5161 gcccctgatg aggacagaga gatgaacaac ctccagctgg ccctggcccg catccagagg
     5221 ggcctgcgct ttgtcaagcg gaccacctgg gatttctgct gtggtctcct gcggcagcgg
     5281 cctcagaagc ccgcagccct tgccgcccag ggccagctgc ccagctgcat tgccaccccc
     5341 tactccccgc cacccccaga gacggagaag gtgcctccca cccgcaagga aacacggttt
     5401 gaggaaggcg agcaaccagg ccagggcacc cccggggatc cagagcccgt gtgtgtgccc
     5461 atcgctgtgg ccgagtcaga cacagatgac caagaagaag atgaggagaa cagcctgggc
     5521 acggaggagg agtccagcaa gcagcaggaa tcccagcctg tgtccggtgg cccagaggcc
     5581 cctccggatt ccaggacctg gagccaggtg tcagcgactg cctcctctga ggccgaggcc
     5641 agtgcatctc aggccgactg gcggcagcag tggaaagcgg aaccccaggc cccagggtgc
     5701 ggtgagaccc cagaggacag ttgctccgag ggcagcacag cagacatgac caacaccgct
     5761 gagctcctgg agcagatccc tgacctcggc caggatgtca aggacccaga ggactgcttc
     5821 actgaaggct gtgtccggcg ctgtccctgc tgtgcggtgg acaccacaca ggccccaggg
     5881 aaggtctggt ggcggttgcg caagacctgc taccacatcg tggagcacag ctggttcgag
     5941 acattcatca tcttcatgat cctactcagc agtggagcgc tggccttcga ggacatctac
     6001 ctagaggagc ggaagaccat caaggttctg cttgagtatg ccgacaagat gttcacatat
     6061 gtcttcgtgc tggagatgct gctcaagtgg gtggcctacg gcttcaagaa gtacttcacc
     6121 aatgcctggt gctggctcga cttcctcatc gtagacgtct ctctggtcag cctggtggcc
     6181 aacaccctgg gctttgccga gatgggcccc atcaagtcac tgcggacgct gcgtgcactc
     6241 cgtcctctga gagctctgtc acgatttgag ggcatgaggg tggtggtcaa tgccctggtg
     6301 ggcgccatcc cgtccatcat gaacgtcctc ctcgtctgcc tcatcttctg gctcatcttc
     6361 agcatcatgg gcgtgaacct ctttgcgggg aagtttggga ggtgcatcaa ccagacagag
     6421 ggagacttgc ctttgaacta caccatcgtg aacaacaaga gccagtgtga gtccttgaac
     6481 ttgaccggag aattgtactg gaccaaggtg aaagtcaact ttgacaacgt gggggccggg
     6541 tacctggccc ttctgcaggt ggcaacattt aaaggctgga tggacattat gtatgcagct
     6601 gtggactcca gggggtatga agagcagcct cagtgggaat acaacctcta catgtacatc
     6661 tattttgtca ttttcatcat ctttgggtct ttcttcaccc tgaacctctt tattggtgtc
     6721 atcattgaca acttcaacca acagaagaaa aagttagggg gccaggacat cttcatgaca
     6781 gaggagcaga agaagtacta caatgccatg aagaagctgg gctccaagaa gccccagaag
     6841 cccatcccac ggcccctgaa caagtaccag ggcttcatat tcgacattgt gaccaagcag
     6901 gcctttgacg tcaccatcat gtttctgatc tgcttgaata tggtgaccat gatggtggag
     6961 acagatgacc aaagtcctga gaaaatcaac atcttggcca agatcaacct gctctttgtg
     7021 gccatcttca caggcgagtg tattgtcaag ctggctgccc tgcgccacta ctacttcacc
     7081 aacagctgga atatcttcga cttcgtggtt gtcatcctct ccatcgtggg cactgtgctc
     7141 tcggacatca tccagaagta cttcttctcc ccgacgctct tccgagtcat ccgcctggcc
     7201 cgaataggcc gcatcctcag actgatccga ggggccaagg ggatccgcac gctgctcttt
     7261 gccctcatga tgtccctgcc tgccctcttc aacatcgggc tgctgctctt cctcgtcatg
     7321 ttcatctact ccatctttgg catggccaac ttcgcttatg tcaagtggga ggctggcatc
     7381 gacgacatgt tcaacttcca gaccttcgcc aacagcatgc tgtgcctctt ccagatcacc
     7441 acgtcggccg gctgggatgg cctcctcagc cccatcctca acactgggcc gccctactgc
     7501 gaccccactc tgcccaacag caatggctct cggggggact gcgggagccc agccgtgggc
     7561 atcctcttct tcaccaccta catcatcatc tccttcctca tcgtggtcaa catgtacatt
     7621 gccatcatcc tggagaactt cagcgtggcc acggaggaga gcaccgagcc cctgagtgag
     7681 gacgacttcg atatgttcta tgagatctgg gagaaatttg acccagaggc cactcagttt
     7741 attgagtatt cggtcctgtc tgactttgcc gatgccctgt ctgagccact ccgtatcgcc
     7801 aagcccaacc agataagcct catcaacatg gacctgccca tggtgagtgg ggaccgcatc
     7861 cattgcatgg acattctctt tgccttcacc aaaagggtcc tgggggagtc tggggagatg
     7921 gacgccctga agatccagat ggaggagaag ttcatggcag ccaacccatc caagatctcc
     7981 tacgagccca tcaccaccac actccggcgc aagcacgaag aggtgtcggc catggttatc
     8041 cagagagcct tccgcaggca cctgctgcaa cgctctttga agcatgcctc cttcctcttc
     8101 cgtcagcagg cgggcagcgg cctctccgaa gaggatgccc ctgagcgaga gggcctcatc
     8161 gcctacgtga tgagtgagaa cttctcccga ccccttggcc caccctccag ctcctccatc
     8221 tcctccactt ccttcccacc ctcctatgac agtgtcacta gagccaccag cgataacctc
     8281 caggtgcggg ggtctgacta cagccacagt gaagatctcg ccgacttccc cccttctccg
     8341 gacagggacc gtgagtccat cgtgggatcc gactacaaag accatgacgg tgattataaa
     8401 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     8461 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     8521 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     8581 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     8641 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     8701 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     8761 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     8821 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     8881 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     8941 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     9001 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     9061 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     9121 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     9181 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     9241 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     9301 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     9361 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     9421 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     9481 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     9541 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     9601 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     9661 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     9721 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     9781 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     9841 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     9901 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     9961 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
    10021 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
    10081 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
    10141 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
    10201 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
    10261 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
    10321 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
    10381 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
    10441 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
    10501 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
    10561 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
    10621 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
    10681 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
    10741 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
    10801 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
    10861 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
    10921 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
    10981 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
    11041 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
    11101 acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
    11161 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    11221 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    11281 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
    11341 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
    11401 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    11461 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
    11521 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    11581 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    11641 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa
    11701 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    11761 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    11821 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    11881 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    11941 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    12001 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    12061 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    12121 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    12181 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    12241 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    12301 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    12361 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    12421 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    12481 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    12541 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    12601 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    12661 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    12721 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    12781 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    12841 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    12901 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    12961 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    13021 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    13081 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    13141 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    13201 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    13261 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    13321 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    13381 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    13441 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    13501 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    13561 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    13621 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    13681 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    13741 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    13801 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    13861 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    13921 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    13981 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    14041 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
    14101 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    14161 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    14221 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    14281 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
