LOCUS       V044762                14158 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044762
VERSION     V044762
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..8184
                     /label="SCN10A(NM_006514)"
                     /note="SCN10A(NM_006514)"
                     /gene="SCN10A"
     CDS             8191..8256
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8300..8511
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8524..8792
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      8821..8830
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8851..9513
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             9583..9636
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9637..10236
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10237..10825
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10899..11132
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11204..11325
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11365..11500
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11533..11549)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11557..11573
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11581..11611)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11626..11647
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11935..12523)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12694..13554)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13555..13659)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14133..14149
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aattccccat tggatccctc
     2341 gaaactaaca acttccgtcg ctttactccg gagtcactgg tggagataga gaagcaaatt
     2401 gctgccaagc agggaacaaa gaaagccaga gagaagcata gggagcagaa ggaccaagaa
     2461 gagaagcctc ggccccagct ggacttgaaa gcctgcaacc agctgcccaa gttctatggt
     2521 gagctcccag cagaactgat cggggagccc ctggaggatc tagatccgtt ctacagcaca
     2581 caccggacat ttatggtgct gaacaaaggg aggaccattt cccggtttag tgccactcgg
     2641 gccctgtggc tattcagtcc tttcaacctg atcagaagaa cggccatcaa agtgtctgtc
     2701 cactcgtggt tcagtttatt tattacggtc actattttgg ttaattgtgt gtgcatgacc
     2761 cgaactgacc ttccagagaa aattgaatat gtcttcactg tcatttacac ctttgaagcc
     2821 ttgataaaga tactggcaag aggattttgt ctaaatgagt tcacgtacct gagagatcct
     2881 tggaactggc tggattttag cgtcattacc ctggcatatg ttggcacagc aatagatctc
     2941 cgtgggatct caggcctgcg gacattcaga gttcttagag cattaaaaac agtttctgtg
     3001 atcccaggcc tgaaggtcat tgtgggggcc ctgattcact cagtgaagaa actggctgat
     3061 gtgaccatcc tcaccatctt ctgcctaagt gtttttgcct tggtggggct gcaactcttc
     3121 aagggcaacc tcaaaaataa atgtgtcaag aatgacatgg ctgtcaatga gacaaccaac
     3181 tactcatctc acagaaaacc agatatctac ataaataagc gaggcacttc tgacccctta
     3241 ctgtgtggca atggatctga ctcaggccac tgccctgatg gttatatctg ccttaaaact
     3301 tctgacaacc cggattttaa ctacaccagc tttgattcct ttgcttgggc tttcctctca
     3361 ctgttccgcc tcatgacaca ggattcctgg gaacgcctct accagcagac cctgaggact
     3421 tctgggaaaa tctatatgat cttttttgtg ctcgtaatct tcctgggatc tttctacctg
     3481 gtcaacttga tcttggctgt agtcaccatg gcgtatgagg agcagaacca ggcaaccact
     3541 gatgaaattg aagcaaagga gaagaagttc caggaggccc tcgagatgct ccggaaggag
     3601 caggaggtgc tagcagcact agggattgac acaacctctc tccactccca caatggatca
     3661 cctttaacct ccaaaaatgc cagtgagaga aggcatagaa taaagccaag agtgtcagag
     3721 ggctccacag aagacaacaa atcaccccgc tctgatcctt acaaccagcg caggatgtct
     3781 tttctaggcc tcgcctctgg aaaacgccgg gctagtcatg gcagtgtgtt ccatttccgg
     3841 tcccctggcc gagatatctc actccctgag ggagtcacag atgatggagt ctttcctgga
     3901 gaccacgaaa gccatcgggg ctctctgctg ctgggtgggg gtgctggcca gcaaggcccc
     3961 ctccctagaa gccctcttcc tcaacccagc aaccctgact ccaggcatgg agaagatgaa
     4021 caccaaccgc cgcccactag tgagcttgcc cctggagctg tcgatgtctc ggcattcgat
     4081 gcaggacaaa agaagacttt cttgtcagca gaatacttag atgaaccttt ccgggcccaa
     4141 agggcaatga gtgttgtcag tatcataacc tccgtccttg aggaactcga ggagtctgaa
     4201 cagaagtgcc caccctgctt gaccagcttg tctcagaagt atctgatctg ggattgctgc
     4261 cccatgtggg tgaagctcaa gacaattctc tttgggcttg tgacggatcc ctttgcagag
     4321 ctcaccatca ccttgtgcat cgtggtgaac accatcttca tggccatgga gcaccatggc
     4381 atgagcccta ccttcgaagc catgctccag ataggcaaca tcgtctttac catatttttt
     4441 actgctgaaa tggtcttcaa aatcattgcc ttcgacccat actattattt ccagaagaag
     4501 tggaatatct ttgactgcat catcgtcact gtgagtctgc tagagctggg cgtggccaag
     4561 aagggaagcc tgtctgtgct gcggagcttc cgcttgctgc gcgtattcaa gctggccaaa
     4621 tcctggccca ccttaaacac actcatcaag atcatcggaa actcagtggg ggcactgggg
     4681 aacctcacca tcatcctggc catcattgtc tttgtctttg ctctggttgg caagcagctc
     4741 ctaggggaaa actaccgtaa caaccgaaaa aatatctccg cgccccatga agactggccc
     4801 cgctggcaca tgcacgactt cttccactct ttcctcattg tcttccgtat cctctgtgga
     4861 gagtggattg agaacatgtg ggcctgcatg gaagttggcc aaaaatccat atgcctcatc
     4921 cttttcttga cggtgatggt gctagggaac ctggtggtgc ttaacctgtt catcgccctg
     4981 ctattgaact ctttcagtgc tgacaacctc acagccccgg aggacgatgg ggaggtgaac
     5041 aacctgcagg tggccctggc acggatccag gtctttggcc atcgtaccaa acaggctctt
     5101 tgcagcttct tcagcaggtc ctgcccattc ccccagccca aggcagagcc tgagctggtg
     5161 gtgaaactcc cactctccag ctccaaggct gagaaccaca ttgctgccaa cactgccagg
     5221 gggagctctg gagggctcca agctcccaga ggccccaggg atgagcacag tgacttcatc
     5281 gctaatccga ctgtgtgggt ctctgtgccc attgctgagg gtgaatctga tcttgatgac
     5341 ttggaggatg atggtgggga agatgctcag agcttccagc aggaagtgat ccccaaagga
     5401 cagcaggagc agctgcagca agtcgagagg tgtggggacc acctgacacc caggagccca
     5461 ggcactggaa catcttctga ggacctggct ccatccctgg gtgagacgtg gaaagatgag
     5521 tctgttcctc aggtccctgc tgagggagtg gacgacacaa gctcctctga gggcagcacg
     5581 gtggactgcc tagatcctga ggaaatcctg aggaagatcc ctgagctggc agatgacctg
     5641 gaagaaccag atgactgctt cacagaagga tgcattcgcc actgtccctg ctgcaaactg
     5701 gataccacca agagtccatg ggatgtgggc tggcaggtgc gcaagacttg ctaccgtatc
     5761 gtggagcaca gctggtttga gagcttcatc atcttcatga tcctgctcag cagtggatct
     5821 ctggcctttg aagactatta cctggaccag aagcccacgg tgaaagcttt gctggagtac
     5881 actgacaggg tcttcacctt tatctttgtg ttcgagatgc tgcttaagtg ggtggcctat
     5941 ggcttcaaaa agtacttcac caatgcctgg tgctggctgg acttcctcat tgtgaatatc
     6001 tcactgataa gtctcacagc gaagattctg gaatattctg aagtggctcc catcaaagcc
     6061 cttcgaaccc ttcgcgctct gcggccactg cgggctcttt ctcgatttga aggcatgcgg
     6121 gtggtggtgg atgccctggt gggcgccatc ccatccatca tgaatgtcct cctcgtctgc
     6181 ctcatcttct ggctcatctt cagcatcatg ggtgtgaacc tcttcgcagg gaagttttgg
     6241 aggtgcatca actataccga tggagagttt tcccttgtac ctttgtcgat tgtgaataac
     6301 aagtctgact gcaagattca aaactccact ggcagcttct tctgggtcaa tgtgaaagtc
     6361 aactttgata atgttgcaat gggttacctt gcacttctgc aggtggcaac ctttaaaggc
     6421 tggatggaca ttatgtatgc agctgttgat tcccgggagg tcaacatgca acccaagtgg
     6481 gaggacaacg tgtacatgta tttgtacttt gtcatcttca tcatttttgg aggcttcttc
     6541 acactgaatc tctttgttgg ggtcataatt gacaacttca atcaacagaa aaaaaagtta
     6601 gggggccagg acatcttcat gacagaggag cagaagaaat actacaatgc catgaagaag
     6661 ttgggctcca agaagcccca gaagcccatc ccacggcccc tgaacaagtt ccagggtttt
     6721 gtctttgaca tcgtgaccag acaagctttt gacatcacca tcatggtcct catctgcctc
     6781 aacatgatca ccatgatggt ggagactgat gaccaaagtg aagaaaagac gaaaattctg
     6841 ggcaaaatca accagttctt tgtggccgtc ttcacaggcg aatgtgtcat gaagatgttc
     6901 gctttgaggc agtactactt cacaaatggc tggaatgtgt ttgacttcat tgtggtggtt
     6961 ctctccattg cgagcctgat tttttctgca attcttaagt cacttcaaag ttacttctcc
     7021 ccaacgctct tcagagtcat ccgcctggcc cgaattggcc gcatcctcag actgatccga
     7081 gcggccaagg ggatccgcac actgctcttt gccctcatga tgtccctgcc tgccctcttc
     7141 aacatcgggc tgttgctatt ccttgtcatg ttcatctact ctatcttcgg tatgtccagc
     7201 tttccccatg tgaggtggga ggctggcatc gacgacatgt tcaacttcca gaccttcgcc
     7261 aacagcatgc tgtgcctctt ccagattacc acgtcggccg gctgggatgg cctcctcagc
     7321 cccatcctca acacagggcc cccctactgt gaccccaatc tgcccaacag caatggcacc
     7381 agaggggact gtgggagccc agccgtaggc atcatcttct tcaccaccta catcatcatc
     7441 tccttcctca tcatggtcaa catgtacatt gcagtgattc tggagaactt caatgtggcc
     7501 acggaggaga gcactgagcc cctgagtgag gacgactttg acatgttcta tgagacctgg
     7561 gagaagtttg acccagaggc cactcagttt attacctttt ctgctctctc ggactttgca
     7621 gacactctct ctggtcccct gagaatccca aaacccaatc gaaatatact gatccagatg
     7681 gacctgcctt tggtccctgg agataagatc cactgcttgg acatcctttt tgctttcacc
     7741 aagaatgtcc taggagaatc cggggagttg gattctctga aggcaaatat ggaggagaag
     7801 tttatggcaa ctaatctttc aaaatcatcc tatgaaccaa tagcaaccac tctccgatgg
     7861 aagcaagaag acatttcagc cactgtcatt caaaaggcct atcggagcta tgtgctgcac
     7921 cgctccatgg cactctctaa caccccatgt gtgcccagag ctgaggagga ggctgcatca
     7981 ctcccagatg aaggttttgt tgcattcaca gcaaatgaaa attgtgtact cccagacaaa
     8041 tctgaaactg cttctgccac atcattccca ccgtcctatg agagtgtcac tagaggcctt
     8101 agtgatagag tcaacatgag gacatctagc tcaatacaaa atgaagatga agccaccagt
     8161 atggagctga ttgcccctgg gcccggatcc gactacaaag accatgacgg tgattataaa
     8221 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     8281 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     8341 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     8401 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     8461 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     8521 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     8581 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     8641 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     8701 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     8761 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     8821 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     8881 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     8941 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     9001 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     9061 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     9121 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     9181 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     9241 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     9301 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     9361 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     9421 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     9481 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     9541 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     9601 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     9661 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     9721 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     9781 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     9841 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     9901 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     9961 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
    10021 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
    10081 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
    10141 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
    10201 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
    10261 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
    10321 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
    10381 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
    10441 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
    10501 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
    10561 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
    10621 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
    10681 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
    10741 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
    10801 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
    10861 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
    10921 acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
    10981 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    11041 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    11101 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
    11161 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
    11221 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    11281 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
    11341 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    11401 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    11461 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa
    11521 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    11581 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    11641 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    11701 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    11761 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    11821 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    11881 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11941 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    12001 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    12061 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    12121 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    12181 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    12241 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    12301 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    12361 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    12421 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    12481 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    12541 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    12601 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    12661 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    12721 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    12781 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    12841 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    12901 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    12961 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    13021 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    13081 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    13141 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    13201 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    13261 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    13321 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    13381 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    13441 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    13501 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    13561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    13621 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    13681 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    13741 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    13801 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    13861 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
    13921 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    13981 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    14041 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    14101 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
