LOCUS       V028828                11959 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028828
VERSION     V028828
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5985
                     /label="Ripor1(NM_001081241)"
                     /note="Ripor1(NM_001081241)"
                     /gene="Ripor1"
     CDS             5992..6057
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6101..6312
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6325..6593
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6622..6631
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6652..7314
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7384..7437
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7438..8037
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8038..8626
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8700..8933
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9005..9126
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9166..9301
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9334..9350)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9358..9374
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9382..9412)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9427..9448
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9736..10324)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10495..11355)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11356..11460)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11934..11950
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga tgtccctgtc ggtgcggccg
     2341 cagcgccgcc tgctcagcgc ccgggtcagt aggagccagt ccttcgcagg cgtcctcggc
     2401 agccacgagc gcgggcccag gagcttcact gtcttcagcc caccagggcc cccacggaag
     2461 cccctagtgc tctcccgagt gtcaaggatg ttttctgtgg cccatccagc tcccaaggtg
     2521 ccgcagccag agaggctgga cctggtgtac actgctctta agcggggact gacggcctac
     2581 ttggaagtgc atcagcagga gcaggagaag ctccagcgcc agataaagga gtccaaaagg
     2641 aattcccgcc taggcttcct gtatgacttg gacaagcaag tcaagtccat tgaacgcttt
     2701 cttcgacgac tggaattcca tgccagtaag attgacgagt tgtatgaagc atactgtgtc
     2761 cagcggcgtc tcagggatgg tgcctacaac atggtccgcg cctatagcac tgggtcccca
     2821 gggagtcgag aggcccgaga cagcctggcc gaggccactc gaggccatcg agagtataca
     2881 gagagcatgt gtcttctgga gaatgagctg gaggcacagt tgggcgagtt tcatctccgg
     2941 atgaaagggc tggctggctt cgccaggctg tgtgtgggcg atcaatatga gatctgcatg
     3001 aaatatggcc gtcagcgctg gaagttgcga ggccggatag agagtagtgg caagcaggtg
     3061 tgggacagtg aagagaccgt ctttcttcct ctactcacag aattcctgtc tatcaaggtg
     3121 acagaattaa agggactggc taaccacgta gtcgtgggca gtgtctcctg tgagaccaag
     3181 gacctgtttg ccgccctgcc ccaggttgtg gctgtggata tcaatgatct tggcaccatc
     3241 aagctaagcc tagaagtcat atggagtccc tttgacaagg atgaccagcc ttcagctgct
     3301 tctacagtca acaaagcttc cacagtcacc aagcgatttt ccacatatag ccagagcccg
     3361 ccagacacac cctcactccg agaacaggcc ttttataaca tgttacggcg acaggaggag
     3421 ctcgagaatg ggacggcctg gtccctgtcc tctgaatcgt cagatgattc atcaagcccg
     3481 cagctctctg gcacagctcg acactcaact cccaagcctc tggtgcaaca gcctgagccc
     3541 ctgcctgtcc aagttgcctt ccgaaggcca gagagcctca cgtctggttc catggatgaa
     3601 gagccagcca tgaccccatc cctggtcaat gggcatgccc cttacagccg gactctcagc
     3661 cacatcagcg aggccagcgt ggatgctgcc ttgactgagg ccgtggaagc tgtggactca
     3721 caaagcccaa tcccagggcc tagcccgctt gtctatccag attccaccca cgtggagcgt
     3781 gtcagtagtg ttctgcctgt cctcaacaat ggccattctg ccacaagtcc cgccctcagt
     3841 acaactggcc ccgcccccac atttatagac cctgccccaa cgacacagct agacttggtt
     3901 cacaaaacca cagactccgc cccttctgag ttgcccagca tcacacatac tacgacaagc
     3961 tctgcatata gtgctgtaag ccttgtcaac agtgttccaa gcctaacttc caccactata
     4021 ggttctgccc acacaaccac gccctctccc ctcacctcca caggctctgt tcccaatgca
     4081 acagactcaa cccaggctac cccaagccct actcacagta ccccaagccc tacccatact
     4141 actatacgcc tcacccacac cactgtaagc cctacacata gctccccgag ccccatacac
     4201 actaccccga gccccaccca taccactgta agtcccacct gcactactcc aagctccggc
     4261 cacagtacca ctagtcccac tcaagaagcc aagatgtcaa ctcataccac cggtgctgtg
     4321 ggcccggtcc agaccactac aagtcccatt tccacaactg aaagcccctc cccttctaca
     4381 gacgtggcta taatctccag ctcctctgca gaatccaccg gcccaggcac tgaacctctg
     4441 ccctgtagtc acccagcctc ccctccctac actaaagcag accccacagc ttcctgcacc
     4501 tcttaccaga gtcttgcttc ctctggttca aaacccctca caagccctgc cccggattcc
     4561 ccagaacaaa tccctaagag cccaagttcc tctccctcat ctagtgcccc tgaaccccaa
     4621 cattcagaac ataacctggc cgcagttgct caagccccag tccctgaggc aacgggagga
     4681 gctggggaca gaaggcttga agaagctctt gggaccctaa tgtctgccct tgatgactat
     4741 cgtggtcagt tccctgagct gcagggcctg gagcaggagg tgactcggct ggagagtctg
     4801 ctcatgcaga gacaaggcct gactcgaagc cgggcttcca gtcttagcat cactgtggag
     4861 catgccctgg agagcttcag cttcctcaat gatgatgaag atgaagacaa cgatggtccc
     4921 ggggacaggc acacaagcag cccagaggtt gtggcagagg acagactaga ctcatcaaat
     4981 ggccagtctc taagcacagg gtgttcagcc ctggatgcta ccttggtcca gcacctgtac
     5041 cactgcagcc gcctcctgct gaaactgggc acatttggac ccctgcgctg ccaggaggcc
     5101 tgggccctgg aacggctgct gcgggaagcg cgtgtattcc aggaagtgtg tgaacgcagc
     5161 aagctatggg ggaactctgc cacatctgcc caggaagtgg tacagttctc cgcctctcgg
     5221 cccggcttcc tgaccttttg ggaccagtgt acagagggac tcagcccctt catctgctct
     5281 gtggagcggg tgctcctcac cttctgcagc cagtacggtg cccgtctttc cctgcgccag
     5341 ccaggcttag ccgaggctgt gtgtgtgaag ttcctggaag atgccctggg acagaagctg
     5401 cccaggaggc cccaaccagg cccgggggag cagttcacca tcttccaatt ctggagttat
     5461 gtagaagcct tggacagccc ctccatggat gcctatgtga cagagactgc agaggaggtg
     5521 ttactagtac agaacctgaa ctctgatgac caggccgttg tgctgaaagc cctaaggtta
     5581 gcaccggagg ggcgcctgcg aaaggatggg cttcgggctc ttagctccct gctggtccac
     5641 ggcaacaaca aagtcatggc tgctgtcagc actcagcttc ggagcctgtc acttggtcct
     5701 gtcttccggg aaagggctct cttgtgcttc ctggaccagc tggaagatgg ggacgtgcag
     5761 actcgagtgg ccggatgcct ggctttgggc tgtatcaagg ctcctgaggg cattgagccc
     5821 ctggtgtact tgtgccaaac agacacagaa gctgtgaggg aagccgcccg gcagagcctg
     5881 cagcagtgcg gggaggaagg acagtctgct catcgacagc tagaggagtc cctggatgct
     5941 ctgccctgca tctttgggcc cagcagcatg gccagcacag cattcggatc cgactacaaa
     6001 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     6061 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     6121 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     6181 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     6241 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     6301 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     6361 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     6421 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     6481 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     6541 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     6601 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     6661 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     6721 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     6781 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     6841 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     6901 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     6961 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     7021 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     7081 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     7141 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     7201 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     7261 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     7321 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     7381 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     7441 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     7501 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     7561 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     7621 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     7681 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     7741 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     7801 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     7861 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7921 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7981 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     8041 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8101 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8161 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8221 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8281 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8341 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     8401 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     8461 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8521 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     8581 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     8641 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8701 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     8761 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8821 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8881 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     8941 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     9001 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9061 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9121 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     9181 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9241 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9301 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     9361 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     9421 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     9481 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9541 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9601 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9661 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9721 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9781 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9841 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9901 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9961 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    10021 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    10081 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    10141 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    10201 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    10261 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    10321 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10381 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    10441 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    10501 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10561 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10621 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10681 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10741 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10801 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10861 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10921 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10981 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    11041 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    11101 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    11161 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    11221 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    11281 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    11341 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    11401 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    11461 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    11521 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    11581 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    11641 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    11701 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    11761 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    11821 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    11881 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    11941 gacggccagt gccaagctg
//
