LOCUS       V032695                11053 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032695
VERSION     V032695
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5079
                     /label="Rb1(NM_009029)"
                     /note="Rb1(NM_009029)"
                     /gene="Rb1"
     CDS             5086..5151
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5195..5406
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5419..5687
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5716..5725
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5746..6408
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6478..6531
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6532..7131
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7132..7720
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7794..8027
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8099..8220
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8260..8395
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8428..8444)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8452..8468
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8476..8506)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8521..8542
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8830..9418)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9589..10449)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10450..10554)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11028..11044
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc cgcccaaagc cccgcgcaga
     2341 gccgcggccg ccgagccccc gccaccgccg ccgccgccgc ctcgggagga cgaccccgcg
     2401 caggacagcg gccccgaaga gctgcccctg gccaggcttg agtttgaaga aattgaagaa
     2461 cccgaattta ttgcattatg tcaaaagtta aaggtacccg atcatgtcag agaaagagct
     2521 tggctaactt gggagaaagt ttcatccgtg gatggaatcc tggaaggata tattcaaaag
     2581 aagaaggaac tctggggcat ctgcatcttt atcgcagcag ttgatctaga tgagatgcca
     2641 ttcactttta ctgagctaca gaaaagcata gaaaccagtg tctataaatt ctttgactta
     2701 ttaaaagaaa tcgataccag taccaaggtt gataatgcta tgtcaagact attgaagaag
     2761 tataatgtgt tatgtgcact ctacagcaaa ttagaacgga cgtgtgaact tatatatttg
     2821 acacaaccca gcagtgcgtt atctactgaa ataaattcta tgttggtgct aaaaatttct
     2881 tggatcactt ttttactagc taaaggagaa gtattacaga tggaagatga cctggtaatc
     2941 tcatttcagc taatgttgtg tgtagttgac tattttatta agttctcacc tcctgcacta
     3001 ctcagagagc catacaaaac agctgcaatc cccattaatg gttcacctcg aacacccaga
     3061 agaggtcaga acaggagcgc tcggatagca aaacaactag aaaatgatac gaggattatc
     3121 gaggttctct gtaaagaaca cgagtgtaat atagatgagg tgaaaaatgt ttatttcaaa
     3181 aattttatcc cttttataaa ttcacttgga attgtatcat ctaatggact tccagaggtt
     3241 gaaagtcttt ctaaacgcta tgaagaagtt tatcttaaaa acaaagattt agatgcaaga
     3301 ctgtttttgg atcatgataa aacacttcag actgatccta tagacagttt tgaaacagag
     3361 agaacgccac gaaaaaacaa ccctgatgaa gaggcaaacg tggttactcc acacactcca
     3421 gttaggactg ttatgaatac tattcaacaa ttaatggtga ttttaaattc tgcaagtgat
     3481 cagccatcag aaaatctgat ttcctacttc aataattgca cagtgaatcc aaaagaaaat
     3541 atcctaaaga gagtaaagga tgttgggcac atctttaaag agaagtttgc taacgctgtg
     3601 ggccagggct gtgttgacat cggagtacag cgatataaac ttggagtccg attgtattac
     3661 cgtgtgatgg aatccatgct taaatcagaa gaagaacgtt tgtccattca gaattttagc
     3721 aaactcctaa atgacaacat ctttcatatg tctttactgg cctgtgctct tgaagttgta
     3781 atggctacgt atagcagaag tacattgcag catcttgatt ctggaacaga tttgtccttc
     3841 ccgtggattc tgaacgtact taatttaaaa gcctttgatt tttacaaagt gattgaaagt
     3901 tttatcaaag tggaagccaa cttgacaaga gaaatgataa aacatttaga aagatgtgag
     3961 catcgaatca tggaatccct tgcatggctt tcagattcac ctttatttga tctcattaag
     4021 cagtccaagg atggagaagg acctgataac cttgaacctg cttgtcctct cagcctgcct
     4081 ctccagggta accatactgc agcagatatg tatctttctc ctctaagatc tccaaagaaa
     4141 agaacttcca ctacacgtgt aaattctgct gcaaatacag agacacaagc agcctcagcc
     4201 ttccatactc agaagccatt gaaatctacc tcccttgccc tgttttacaa aaaagtgtac
     4261 cgtctagcat atctccgact aaatacactc tgtgcacgcc ttctgtctga ccacccagag
     4321 ctagagcaca tcatctggac tctgtttcag catacattgc aaaatgagta tgagctcatg
     4381 agagaccgac atttggacca gattatgatg tgctctatgt atggcatctg caaggtgaag
     4441 aacatcgacc tcaagttcaa aatcatcgtc actgcctaca aggatcttcc tcacgctgcc
     4501 caggagacct ttaaacgtgt tttgatcaga gaagaggagt ttgattccat tatagtattc
     4561 tataactccg ttttcatgca gagactaaaa acaaatattt tacagtatgc ctccaccagg
     4621 cctcctacct tgtcaccaat acctcacatt cctcgaagcc cttacaagtt ttctagttca
     4681 cccttacgga ttcctggagg taacatctat atatcacccc taaagagtcc ttataaaatt
     4741 tcagaaggtc tgccaacacc cacaaaaatg actccgagat caagaatctt ggtctcaatt
     4801 ggtgaatcat ttgggacatc tgaaaagttc cagaaaataa accagatggt gtgtaatagt
     4861 gacagagtgc tcaaaagaag tgctgaaggc ggcaaccccc ccaaaccact gaaaaagctg
     4921 cgctttgaca tcgagggagc cgatgaagca gatgggagta aacatctccc agcggagtcc
     4981 aaattccaac agaaactggc agaaatgact tccactcgaa cacgaatgca aaagcagaga
     5041 atgaatgaga gcaaggatgt ctcaaacaag gaggaaaagg gatccgacta caaagaccat
     5101 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     5161 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     5221 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     5281 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     5341 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     5401 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     5461 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     5521 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     5581 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     5641 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     5701 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     5761 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     5821 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     5881 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     5941 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     6001 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     6061 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     6121 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     6181 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     6241 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     6301 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     6361 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     6421 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
     6481 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
     6541 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6601 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6661 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6721 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6781 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     6841 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6901 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6961 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     7021 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     7081 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
     7141 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7201 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7261 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7321 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7381 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7441 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7501 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7561 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7621 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7681 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     7741 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7801 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     7861 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7921 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7981 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8041 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     8101 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8161 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8221 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     8281 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8341 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     8401 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     8461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9241 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10561 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10621 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10681 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10741 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10801 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10861 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    10921 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10981 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    11041 cagtgccaag ctg
//
