LOCUS       V028065                10696 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028065
VERSION     V028065
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4722
                     /label="RSBN1(NM_018364)"
                     /note="RSBN1(NM_018364)"
                     /gene="RSBN1"
     CDS             4729..4794
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4838..5049
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5062..5330
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5359..5368
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5389..6051
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6121..6174
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6175..6774
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6775..7363
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7437..7670
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7742..7863
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7903..8038
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8071..8087)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8095..8111
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8119..8149)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8164..8185
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8473..9061)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9232..10092)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10093..10197)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10671..10687
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt tcatctctgg acgaagaacg
     2341 gccgacaagt ggagggcgga ggagagactc caatgcccag cgggcagtgc gcgggcggcg
     2401 cttgcgcgat gcgcggacgg gggggcggtc gggccattta aatgtgtgtt tgtgggtgaa
     2461 atggctgcgc aggtcggagc ggtgcgcgta gtacgggcgg tggcggcgca ggaggagccg
     2521 gacaaagagg ggaaggagaa acctcatgct ggggtctccc cgcggggagt taaacggcag
     2581 cgccgatcta gcagtggggg gtctcaggag aagcgggggc ggccgagcca ggagccccct
     2641 ctcgctcccc ctcaccggcg gcgtcgcagc cgccaacatc ctgggccgct gccgccaacg
     2701 aatgcagccc caactgtccc aggccctgtt gagcctcttc tcctgccgcc tccgccgcca
     2761 ccttcgctgg cacccgccgg gcccgctgtc gctgcccctc tcccggcccc aagcacctcg
     2821 gccctcttca ccttctcgcc tctgacggtg agcgcggccg ggcccaagca taagggccac
     2881 aaggagcggc acaagcacca tcaccaccgc ggccccgatg gtgatcccag ctcctgcgga
     2941 accgatctca agcacaagga caagcaggaa aacggcgaga ggactggagg ggtgcctctg
     3001 atcaaagccc ccaagagaga aacaccagat gaaaatggta aaacccagag agccgatgat
     3061 tttgtcttga agaaaataaa gaagaaaaag aaaaagaaac accgagaaga catgcgagga
     3121 agacgcctta aaatgtacaa taaggaagta caaaccgtct gtgctggcct gacccgcatc
     3181 agtaaagaaa ttctcaccca aggacaaata aatagcactt caggacttaa taaggagtcc
     3241 ttcaggtatc tgaaagatga acagctgtgc cgattaaatt tgggtatgca agaatatcgg
     3301 gtaccccagg gagtacaaac accttttatg actcaccagg aacattctat tcgtagaaat
     3361 ttcttaaaaa caggtactaa atttagcaac tttattcatg aggaacacca gtccaatggt
     3421 ggtgctcttg tccttcatgc ttacatggat gaactctcat ttttgtctcc aatggagatg
     3481 gagagatttt ctgaggagtt tcttgctttg acattcagtg aaaatgagaa aaatgctgct
     3541 tactatgctt tagcaatagt gcatggagcg gctgcttatc tcccagactt cttggactac
     3601 tttgctttta atttccccaa cactccagtg aaaatggaaa ttctgggcaa gaaagatatt
     3661 gaaacaacca ccatttcaaa ttttcacact caggtcaaca ggacatactg ctgtggcacc
     3721 taccgagcag gtcctatgcg gcagataagt ctcgttggag cagtagatga agaagttggt
     3781 gattatttcc cagagttcct tgatatgtta gaagaatcac catttctgaa aatgactttg
     3841 ccctggggta cactttctag cctccgactc cagtgtaggt cccagagtga tgatgggcct
     3901 ataatgtggg taaggccagg agaacagatg atccctacag cagatatgcc aaagtcaccc
     3961 ttcaaaagac gacgatcaat gaatgaaata aaaaatctcc agtacctacc tcggaccagt
     4021 gaaccccgcg aagttctctt tgaagatagg actagagctc atgctgatca tgtcggtcag
     4081 gggtttgact ggcagagtac ggctgctgtt ggagttttga aagctgtaca atttggtgaa
     4141 tggagtgacc aacctcgcat aaccaaagat gtgatttgtt ttcatgctga ggattttact
     4201 gatgttgtac aaagacttca gttagatctt catgaacctc cagtttccca gtgcgtacag
     4261 tgggtagatg aagctaaact aaaccaaatg aggcgggaag gcattcgtta tgctagaatt
     4321 cagctttgcg acaatgatat ctacttcatc cctagaaatg tcattcatca gttcaaaaca
     4381 gtttcggcgg tgtgcagctt agcctggcat ataaggctta aacagtacca ccctgttgtg
     4441 gaagccactc aaaacacaga aagcaattct aacatggact gtggtttaac tggaaagcga
     4501 gaattagaag ttgactccca atgtgtgagg ataaaaactg aatctgaaga agcatgcaca
     4561 gagattcagc tgttaacaac tgcttcatca tctttcccac ctgcatcaga acttaatcta
     4621 cagcaagatc agaagactca gcctattcca gttttaaaag tggaaagtag actggactct
     4681 gaccagcaac acaatctgca agaacattca accacttctg tgggatccga ctacaaagac
     4741 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     4801 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     4861 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     4921 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     4981 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     5041 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     5101 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     5161 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     5221 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     5281 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     5341 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     5401 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     5461 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     5521 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     5581 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     5641 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     5701 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     5761 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     5821 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     5881 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     5941 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     6001 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     6061 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     6121 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     6181 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6241 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6301 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6361 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6421 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6481 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6541 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6601 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6661 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6721 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     6781 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6841 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6901 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6961 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7021 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7081 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7141 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7201 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7261 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7321 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     7381 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7441 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     7501 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7561 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7621 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7681 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7741 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7801 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7861 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     7921 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7981 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8041 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     8101 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8161 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8221 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8281 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8881 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9121 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9181 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9241 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9301 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9361 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9421 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9481 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9541 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9601 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9661 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9721 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9781 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9841 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9901 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9961 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10021 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10081 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10141 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10201 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10261 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    10321 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10381 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    10441 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10501 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    10561 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10621 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    10681 ggccagtgcc aagctg
//
