LOCUS       V039276                11476 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039276
VERSION     V039276
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6306
                     /label="RPTOR(NM_020761)"
                     /note="RPTOR(NM_020761)"
                     /gene="RPTOR"
     CDS             6313..6402
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        6446..6657
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6670..6938
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6965..7564
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7571..8159
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8233..8466
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8538..8659
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8678..8813
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8851..8867)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8875..8891
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8899..8929)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8944..8965
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9253..9841)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10012..10872)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10873..10977)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11451..11467
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggagtcc gaaatgctgc aatcgcctct tctgggcctg
     2341 ggggaggaag atgaggctga tcttacagac tggaacctac ctttggcttt tatgaaaaag
     2401 aggcactgtg agaaaattga aggctccaaa tccttagctc agagctggag gatgaaggat
     2461 cggatgaaga cagtcagtgt tgccttagtt ttgtgcctga atgttggtgt ggaccctccc
     2521 gatgtggtga agaccacgcc ctgtgcacgc ttggaatgct ggatcgatcc tctgtcgatg
     2581 ggtcctcaga aagctctgga aaccatcggt gcaaatttac agaagcagta cgagaactgg
     2641 cagccaaggg cccggtacaa gcagagcctt gacccaactg tggatgaagt caagaagctc
     2701 tgcacgtcct tacgtcgcaa cgccaaggag gagcgagtcc tctttcacta caatggccac
     2761 ggggtgcccc ggcccacagt caacggggag gtctgggtct tcaacaagaa ctacacgcag
     2821 tacatccctc tgtccatata tgacctgcag acgtggatgg gcagcccgtc gatcttcgtc
     2881 tacgactgct ccaatgctgg cttgatcgtc aagtccttca agcagttcgc actacagcgg
     2941 gagcaggagc tggaggtagc tgcaatcaac ccaaatcacc ctcttgctca gatgcctttg
     3001 cctccgtcga tgaaaaactg catccagctg gcagcctgcg aggccaccga gctgctgccc
     3061 atgatccccg acctcccggc tgacctattc acctcctgcc tcaccacccc catcaagatc
     3121 gccctgcgct ggttttgcat gcagaaatgt gtcagtctgg tgcctggcgt cacactggat
     3181 ttgatagaaa agatccctgg ccgcctgaac gacaggagga cgcccctggg tgaactgaac
     3241 tggatcttca cagccatcac agacaccatc gcgtggaacg tgctcccccg ggatctcttc
     3301 caaaagctct tcagacagga cttgctggtg gctagtctgt ttcgaaattt tttattggcg
     3361 gaaaggatta tgaggtcgta taactgcact cccgtcagca gcccgcgtct gccgcccacg
     3421 tacatgcacg ccatgtggca agcctgggac ctggctgttg acatctgtct gtctcagctg
     3481 ccgacgatca tcgaggaagg cactgcgttt cggcacagcc cgttcttcgc cgagcagctg
     3541 accgcattcc aggtgtggct caccatgggc gtggagaacc gaaacccacc cgaacagctg
     3601 cccatcgtcc tgcaggtgct gttaagccaa gtgcaccggc tgagagcatt ggacttgctt
     3661 ggaagatttt tggacctggg tccctgggca gtgagcctgg ccttgtctgt cggcatcttc
     3721 ccctacgtgc tgaagctgct ccagagctcg gcccgagagc tgcggccact tctcgttttc
     3781 atctgggcca agatcctcgc agtggacagc tcgtgccaag cggacctcgt gaaggacaac
     3841 ggccacaagt acttcctgtc ggtcctggcg gacccctaca tgccagctga acaccggacc
     3901 atgacggctt tcattctcgc cgtgatcgtc aacagctatc acacggggca ggaagcctgc
     3961 cttcagggaa acctcattgc catctgcctg gagcagctca acgacccgca ccccttgctg
     4021 cgccagtggg tggccatctg cctcggcagg atctggcaga acttcgactc ggcgaggtgg
     4081 tgcggcgtga gggacagcgc tcatgagaag ctctacagcc tcctctccga ccccattccc
     4141 gaggtccgct gcgcagcggt cttcgccctt ggcacgttcg tgggcaactc tgcagagagg
     4201 acggaccact ccaccaccat cgaccacaac gtggccatga tgctggccca gctggtcagc
     4261 gacgggagcc ccatggtccg gaaggagctg gtggtggctc tgagtcatct tgtggttcag
     4321 tatgaaagca atttctgcac cgtggccctg cagttcatag aagaggaaaa gaactacgcc
     4381 ttgccttctc cagcaaccac agagggaggg agtttgaccc cagtgcgaga cagcccgtgc
     4441 acccccagac ttcgttctgt gagctcctat ggaaacatcc gtgctgtcgc cacagccagg
     4501 agcctcaaca aatctttgca gaacctgagt ttgacagagg aatctggtgg cgcggtggcg
     4561 ttctcccccg gaaacctcag caccagcagc agcgccagca gcaccctggg cagccccgag
     4621 aatgaggagc atatcctgtc cttcgagacc atcgacaaga tgcgccgcgc cagctcctac
     4681 tcctccctca actccctcat cggagtttcc tttaacagtg tttacactca gatttggaga
     4741 gtcctgctgc acctggctgc tgacccctat ccagaggtct cggacgtggc catgaaagta
     4801 ctcaacagca tcgcctacaa ggccaccgtg aacgcccggc cgcagcgcgt cctggacacc
     4861 tcctccctca cgcagtcggc ccccgccagc cccaccaaca agggcgtgca catccaccag
     4921 gcggggggct cccctccggc gtccagcacc agcagctcca gcctgaccaa cgatgtggcc
     4981 aagcagccgg tcagccgaga cttgccttct ggccggccgg gcaccacagg ccccgctggg
     5041 gcgcagtaca cccctcactc ccaccagttc ccccggacac ggaagatgtt cgacaagggc
     5101 ccagagcaga ctgcggacga cgcggacgat gctgctggac acaaaagttt catctccgcc
     5161 acggtgcaga cggggttctg cgactggagc gcccgctatt ttgcccagcc cgtcatgaag
     5221 atcccagaag agcacgacct ggagagtcag atccgcaagg agcgggagtg gcggttcctg
     5281 cgaaacagcc gtgtcaggag gcaggcccag caagtcattc agaagggcat tacgagattg
     5341 gacgaccaaa tatttctgaa caggaacccc ggcgtcccct ctgtggtgaa attccacccc
     5401 ttcacgccgt gcatcgccgt agccgacaag gacagcatct gcttttggga ctgggagaaa
     5461 ggggagaagc tggattattt ccacaatggg aaccctcggt acacgagggt cactgccatg
     5521 gagtatctga acggccagga ctgctcgctt ctgctgacgg ccacagacga tggtgccatc
     5581 agggtctgga agaattttgc tgatttggaa aagaacccag agatggtgac cgcgtggcag
     5641 gggctctcgg acatgctgcc aacgacgcga ggagctggga tggtggtgga ctgggagcag
     5701 gagaccggcc tcctcatgag ctcaggagac gtgcggatcg tccggatctg ggacacagac
     5761 cgtgagatga aggtgcagga catccctacg ggcgcagaca gctgtgtgac gagtctgtcc
     5821 tgtgattccc accgctcact catcgtggct ggcctcggtg acggctccat ccgcgtctac
     5881 gacagaagga tggcactcag cgaatgccgc gtcatgacgt accgggagca cacagcctgg
     5941 gtggtgaagg cctccctgca gaagcgtccc gacggccaca tcgtgagtgt gagcgtcaat
     6001 ggagatgtgc gcatctttga tccccggatg cctgagtcgg taaatgtgct tcagatcgtg
     6061 aaggggctga cggccctgga catccacccc caggcggacc tgatcgcatg tggctccgtc
     6121 aatcagttca ccgccatcta caacagcagc ggagagctca tcaacaacat caagtactac
     6181 gacggcttca tgggccagcg ggtcggcgcc atcagctgcc tggccttcca cccgcactgg
     6241 cctcacctgg ccgtgggaag caacgactac tacatctccg tgtactcggt ggagaagcgt
     6301 gtcagaggat ccgaacaaaa actaatatca gaggaagatt tggaacagaa actaatcagt
     6361 gaagaagatt tagagcaaaa gttaatttcc gaggaggact tatagcggcc gcgaaggatc
     6421 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     6481 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     6541 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     6601 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     6661 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     6721 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     6781 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     6841 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     6901 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     6961 ctagatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     7021 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     7081 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     7141 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     7201 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     7261 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     7321 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     7381 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     7441 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     7501 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     7561 ctgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7621 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7681 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7741 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7801 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7861 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7921 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     7981 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     8041 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     8101 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     8161 tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8221 aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt
     8281 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8341 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8401 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8461 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     8521 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8581 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8641 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag
     8701 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8761 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt
     8821 ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8881 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8941 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9001 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9061 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9241 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9661 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10021 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10081 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10141 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10201 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10261 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10321 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10381 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10441 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10501 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10561 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10621 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10681 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10741 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10801 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10861 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10921 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10981 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    11041 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    11101 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    11161 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    11221 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    11281 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    11341 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    11401 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    11461 ggccagtgcc aagctg
//
