LOCUS       V041555                10626 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041555
VERSION     V041555
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4645
                     /label="RNF43(NM_017763)"
                     /note="RNF43(NM_017763)"
                     /gene="RNF43"
     CDS             4652..4741
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4785..4996
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5009..5277
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5306..5315
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5336..5998
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6068..6121
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6122..6721
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6722..7309
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7383..7616
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7688..7809
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7828..7963
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8001..8017)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8025..8041
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8049..8079)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8094..8115
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8403..8991)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9162..10022)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10023..10127)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10601..10617
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatga gtggtggcca ccagctgcag ctggctgccc tctggccctg
     2341 gctgctgatg gctaccctgc aggcaggctt tggacgcaca ggactggtac tggcagcagc
     2401 ggtggagtct gaaagatcag cagaacagaa agctattatc agagtgatcc ccttgaaaat
     2461 ggaccccaca ggaaaactga atctcacttt ggaaggtgtg tttgctggtg ttgctgaaat
     2521 aactccagca gaaggaaaat taatgcagtc ccacccgctg tacctgtgca atgccagtga
     2581 tgacgacaat ctggagcctg gattcatcag catcgtcaag ctggagagtc ctcgacgggc
     2641 cccccgcccc tgcctgtcac tggctagcaa ggctcggatg gcgggtgagc gaggagccag
     2701 tgctgtcctc tttgacatca ctgaggatcg agctgctgct gagcagctgc agcagccgct
     2761 ggggctgacc tggccagtgg tgttgatctg gggtaatgac gctgagaagc tgatggagtt
     2821 tgtgtacaag aaccaaaagg cccatgtgag gattgagctg aaggagcccc cggcctggcc
     2881 agattatgat gtgtggatcc taatgacagt ggtgggcacc atctttgtga tcatcctggc
     2941 ttcggtgctg cgcatccggt gccgcccccg ccacagcagg ccggatccgc ttcagcagag
     3001 aacagcctgg gccatcagcc agctggccac caggaggtac caggccagct gcaggcaggc
     3061 ccggggtgag tggccagact cagggagcag ctgcagctca gcccctgtgt gtgccatctg
     3121 tctggaggag ttctctgagg ggcaggagct acgggtcatt tcctgcctcc atgagttcca
     3181 tcgtaactgt gtggacccct ggttacatca gcatcggact tgccccctct gcatgttcaa
     3241 catcacagag ggagattcat tttcccagtc cctgggaccc tctcgatctt accaagaacc
     3301 aggtcgaaga ctccacctca ttcgccagca tcccggccat gcccactacc acctccctgc
     3361 tgcctacctg ttgggccctt cccggagtgc agtggctcgg cccccacgac ctggtccctt
     3421 cctgccatcc caggagccag gcatgggccc tcggcatcac cgcttcccca gagctgcaca
     3481 tccccgggct ccaggagagc agcagcgcct ggcaggagcc cagcacccct atgcacaagg
     3541 ctggggactg agccacctcc aatccacctc acagcaccct gctgcttgcc cagtgcccct
     3601 acgccgggcc aggccccctg acagcagtgg atctggagaa agctattgca cagaacgcag
     3661 tgggtacctg gcagatgggc cagccagtga ctccagctca gggccctgtc atggctcttc
     3721 cagtgactct gtggtcaact gcacggacat cagcctacag ggggtccatg gcagcagttc
     3781 tactttctgc agctccctaa gcagtgactt tgacccccta gtgtactgca gccctaaagg
     3841 ggatccccag cgagtggaca tgcagcctag tgtgacctct cggcctcgtt ccttggactc
     3901 ggtggtgccc acaggggaaa cccaggtttc cagccatgtc cactaccacc gccaccggca
     3961 ccaccactac aaaaagcggt tccagtggca tggcaggaag cctggcccag aaaccggagt
     4021 cccccagtcc aggcctccta ttcctcggac acagccccag ccagagccac cttctcctga
     4081 tcagcaagtc accagatcca actcagcagc cccttcgggg cggctctcta acccacagtg
     4141 ccccagggcc ctccctgagc cagcccctgg cccagttgac gcctccagca tctgccccag
     4201 taccagcagt ctgttcaact tgcaaaaatc cagcctctct gcccgacacc cacagaggaa
     4261 aaggcggggg ggtccctccg agcccacccc tggctctcgg ccccaggatg caactgtgca
     4321 cccagcttgc cagatttttc cccattacac ccccagtgtg gcatatcctt ggtccccaga
     4381 ggcacacccc ttgatctgtg gacctccagg cctggacaag aggctgctac cagaaacccc
     4441 aggcccctgt tactcaaatt cacagccagt gtggttgtgc ctgactcctc gccagcccct
     4501 ggaaccacat ccacctgggg aggggccttc tgaatggagt tctgacaccg cagagggcag
     4561 gccatgccct tatccgcact gccaggtgct gtcggcccag cctggctcag aggaggaact
     4621 cgaggagctg tgtgaacagg ctgtgggatc cgaacaaaaa ctaatatcag aggaagattt
     4681 ggaacagaaa ctaatcagtg aagaagattt agagcaaaag ttaatttccg aggaggactt
     4741 atagcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
     4801 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
     4861 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     4921 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     4981 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg
     5041 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     5101 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     5161 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     5221 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa
     5281 gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc
     5341 catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg
     5401 cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg
     5461 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt
     5521 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta
     5581 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta
     5641 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga
     5701 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca
     5761 ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg
     5821 cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag
     5881 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa
     5941 caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag
     6001 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg
     6061 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     6121 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6181 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6241 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6301 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6361 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6421 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6481 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6541 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6601 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6661 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6721 aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6781 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6841 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6901 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6961 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7021 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7081 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7141 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7201 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7261 ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc tggtaccttt
     7321 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7381 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc
     7441 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7501 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7561 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7621 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7681 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7741 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7801 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat
     7861 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7921 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     7981 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8041 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8101 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8161 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8221 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8281 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8341 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8401 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8461 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8521 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8581 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8641 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8701 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8761 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8821 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     8881 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8941 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9001 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9061 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9121 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9181 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9241 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9301 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9361 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9421 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9481 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9541 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9601 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9661 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9721 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9781 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9841 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9901 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9961 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10021 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10081 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10141 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    10201 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
    10261 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
    10321 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
    10381 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    10441 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    10501 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    10561 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
    10621 aagctg
//
