LOCUS       V032039                10133 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032039
VERSION     V032039
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4314
                     /label="RIPK1(NM_003804)"
                     /note="RIPK1(NM_003804)"
                     /gene="RIPK1"
     CDS             4351..5061
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        5103..5314
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5327..5595
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5622..6221
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6228..6816
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6890..7123
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7195..7316
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7335..7470
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7508..7524)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7532..7548
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7556..7586)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7601..7622
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7910..8498)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8669..9529)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9530..9634)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10108..10124
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcaacca gacatgtcct tgaatgtcat taagatgaaa
     2341 tccagtgact tcctggagag tgcagaactg gacagcggag gctttgggaa ggtgtctctg
     2401 tgtttccaca gaacccaggg actcatgatc atgaaaacag tgtacaaggg gcccaactgc
     2461 attgagcaca acgaggccct cttggaggag gcgaagatga tgaacagact gagacacagc
     2521 cgggtggtga agctcctggg cgtcatcata gaggaaggga agtactccct ggtgatggag
     2581 tacatggaga agggcaacct gatgcacgtg ctgaaagccg agatgagtac tccgctttct
     2641 gtaaaaggaa ggataatttt ggaaatcatt gaaggaatgt gctacttaca tggaaaaggc
     2701 gtgatacaca aggacctgaa gcctgaaaat atccttgttg ataatgactt ccacattaag
     2761 atcgcagacc tcggccttgc ctcctttaag atgtggagca aactgaataa tgaagagcac
     2821 aatgagctga gggaagtgga cggcaccgct aagaagaatg gcggcaccct ctactacatg
     2881 gcgcccgagc acctgaatga cgtcaacgca aagcccacag agaagtcgga tgtgtacagc
     2941 tttgctgtag tactctgggc gatatttgca aataaggagc catatgaaaa tgctatctgt
     3001 gagcagcagt tgataatgtg cataaaatct gggaacaggc cagatgtgga tgacatcact
     3061 gagtactgcc caagagaaat tatcagtctc atgaagctct gctgggaagc gaatccggaa
     3121 gctcggccga catttcctgg cattgaagaa aaatttaggc ctttttattt aagtcaatta
     3181 gaagaaagtg tagaagagga cgtgaagagt ttaaagaaag agtattcaaa cgaaaatgca
     3241 gttgtgaaga gaatgcagtc tcttcaactt gattgtgtgg cagtaccttc aagccggtca
     3301 aattcagcca cagaacagcc tggttcactg cacagttccc agggacttgg gatgggtcct
     3361 gtggaggagt cctggtttgc tccttccctg gagcacccac aagaagagaa tgagcccagc
     3421 ctgcagagta aactccaaga cgaagccaac taccatcttt atggcagccg catggacagg
     3481 cagacgaaac agcagcccag acagaatgtg gcttacaaca gagaggagga aaggagacgc
     3541 agggtctccc atgacccttt tgcacagcaa agaccttacg agaattttca gaatacagag
     3601 ggaaaaggca ctgcttattc cagtgcagcc agtcatggta atgcagtgca ccagccctca
     3661 gggctcacca gccaacctca agtactgtat cagaacaatg gattatatag ctcacatggc
     3721 tttggaacaa gaccactgga tccaggaaca gcaggtccca gagtttggta caggccaatt
     3781 ccaagtcata tgcctagtct gcataatatc ccagtgcctg agaccaacta tctaggaaat
     3841 acacccacca tgccattcag ctccttgcca ccaacagatg aatctataaa atataccata
     3901 tacaatagta ctggcattca gattggagcc tacaattata tggagattgg tgggacgagt
     3961 tcatcactac tagacagcac aaatacgaac ttcaaagaag agccagctgc taagtaccaa
     4021 gctatctttg ataataccac tagtctgacg gataaacacc tggacccaat cagggaaaat
     4081 ctgggaaagc actggaaaaa ctgtgcccgt aaactgggct tcacacagtc tcagattgat
     4141 gaaattgacc atgactatga gcgagatgga ctgaaagaaa aggtttacca gatgctccaa
     4201 aagtgggtga tgagggaagg cataaaggga gccacggtgg ggaagctggc ccaggcgctc
     4261 caccagtgtt ccaggatcga ccttctgagc agcttgattt acgtcagcca gaacggatcc
     4321 ggtggaggcg gatcaggagg cggtggatct atggtgagca agggcgagga ggataacatg
     4381 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     4441 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     4501 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     4561 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     4621 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     4681 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     4741 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     4801 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     4861 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     4921 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     4981 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     5041 atggacgagc tgtacaagta ggcggccgcg aaggatctgc gatcgctccg gtgcccgtca
     5101 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     5161 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     5221 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     5281 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     5341 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     5401 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     5461 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     5521 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     5581 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gatgaccgag tacaagccca
     5641 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     5701 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     5761 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5821 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5881 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     5941 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6001 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6061 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6121 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6181 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat caacctctgg
     6241 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6301 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6361 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6421 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6481 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6541 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6601 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6661 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6721 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6781 agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag accaatgact
     6841 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     6901 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
     6961 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7021 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7081 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7141 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7201 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7261 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7321 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7381 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7441 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
     7501 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7561 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7621 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7681 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7741 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7801 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7861 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7921 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7981 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8041 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8101 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8161 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8221 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8281 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8341 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8401 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8461 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8521 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8581 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8641 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8701 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8761 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8821 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8881 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8941 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9001 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9061 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9121 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9181 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9241 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9301 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9361 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9421 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9481 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9541 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9601 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9661 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     9721 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     9781 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     9841 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     9901 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     9961 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    10021 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    10081 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
