LOCUS       V042831                10537 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042831
VERSION     V042831
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4563
                     /label="RFX3(NM_134428)"
                     /note="RFX3(NM_134428)"
                     /gene="RFX3"
     CDS             4570..4635
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4679..4890
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4903..5171
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5200..5209
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5230..5892
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5962..6015
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6016..6615
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6616..7204
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7278..7511
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7583..7704
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7744..7879
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7912..7928)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7936..7952
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7960..7990)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8005..8026
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8314..8902)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9073..9933)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9934..10038)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10512..10528
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc agacatcaga gactgggtcg
     2341 gacacaggct cgacagtgac cttacaaaca tctgtggcta gtcaagcagc agtgcctacg
     2401 caggtggtac agcaagtacc agtacaacaa caggtacagc aggtacagac tgtgcagcag
     2461 gtacaacatg tctatcccgc tcaggtgcag tatgtggaag gaagcgatac tgtctatacc
     2521 aatggagcaa tccgaacaac aacgtatcct tacacagaga cacagatgta cagccaaaat
     2581 actggaggga attactttga tactcaaggg agttccgccc aggtgactac cgtggtctca
     2641 tcccacagta tggtgggcac tggtgggatt cagatgggcg tcacaggagg acaactcatc
     2701 agcagctctg gaggaaccta tctgatcggc aactcaatgg agaattctgg tcactcagtg
     2761 acacacacaa ctcgggcctc cccagcgaca attgaaatgg cgattgagac gctgcaaaag
     2821 tctgacggtc tgtccactca cagaagctct cttctcaaca gccatctcca gtggctgttg
     2881 gacaattatg agacagcaga aggagtgagc cttcccagaa gcactctgta caaccactac
     2941 cttcgacact gtcaggaaca caaactggac ccagtcaatg ctgcctcttt tggaaaatta
     3001 ataagatcaa tttttatggg gctacgaacc aggagattgg gcactagagg aaactccaaa
     3061 taccactact atgggattcg tgtcaagcca gattcccctc ttaatcgtct gcaagaagac
     3121 atgcagtata tggctatgag acaacaaccc atgcaacaga aacaaaggta caagcctatg
     3181 cagaaagtgg atggggttgc agatggtttc acaggaagtg gtcaacagac aggcacatct
     3241 gttgagcaaa ctgtaattgc ccaaagccaa catcatcaac agtttttaga tgcatctcga
     3301 gcacttccag agtttggaga agttgaaatc tcttctctgc cagatggtac tacctttgag
     3361 gatatcaagt cactgcagag tctttataga gagcactgtg aggcaatatt ggacgttgtt
     3421 gtgaatcttc aatttagcct gatagaaaaa ttgtggcaaa cattctggcg ctattctccc
     3481 tctactccaa ctgatggcac taccattacc gaatcgagca atctgagtga aatagaaagt
     3541 cgacttccga aagcaaagct gataactctg tgcaaacatg agtctatcct gaaatggatg
     3601 tgtaactgtg accatgggat gtaccaggct ttggtggaga ttctcatccc cgacgtcctt
     3661 agacctattc ctagtgcctt gacccaagcc attcgaaatt ttgcaaaaag ccttgaaggt
     3721 tggctttcca atgccatgaa caatattcca cagagaatga tacaaaccaa ggttgccgct
     3781 gtaagtgcct ttgcccagac tctgcgaaga tacacgtcgc ttaatcacct ggcccaggca
     3841 gctcgtgcag tgcttcagaa cacttcccaa atcaaccaga tgcttagtga cctcaaccgt
     3901 gtcgactttg ccaatgtcca ggagcaggct tcctgggtgt gccagtgtga tgacaacatg
     3961 gttcagagac tagaaacaga cttcaagatg actcttcagc agcagagcac cctggagcag
     4021 tgggctgcgt ggcttgacaa tgtgatgatg caagcactga aaccctatga aggaagaccc
     4081 agttttccta aagccgccag gcagtttctg ctaaaatggt ctttctacag ctcaatggtt
     4141 attcgggact taaccttacg cagtgctgct agctttggct ccttccacct gatccgtcta
     4201 ctctacgacg aatatatgtt ttacttagta gaacatcgtg ttgctcaggc aacaggagag
     4261 actcctatag cagtcatggg cgagtttggt gatttaaatg ccgtgtctcc tggaaatctg
     4321 gataaagatg aaggcagtga agtagaaagt gaaatggatg aagaactgga tgactcttca
     4381 gagcctcaag ccaaaagaga gaaaacagag ctgagccagg catttccagt gggctgcatg
     4441 cagcctgttc tcgagactgg cgtgcaacca agcctcctga atccaattca cagcgagcac
     4501 attgtcacaa gtactcagac tatcagacag tgcagcgcta caggaaatac ctacactgca
     4561 gtcggatccg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     4621 gatgacgatg acaagtagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     4681 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     4741 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     4801 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     4861 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     4921 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     4981 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     5041 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     5101 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     5161 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag
     5221 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag
     5281 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg
     5341 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac
     5401 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc
     5461 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac
     5521 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc
     5581 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc
     5641 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc
     5701 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc
     5761 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag
     5821 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc
     5881 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga
     5941 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag
     6001 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6061 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6121 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6181 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6241 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6301 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6361 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6421 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6481 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6541 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6601 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6661 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6721 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6781 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6841 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6901 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6961 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     7021 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7081 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7141 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7201 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7261 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt
     7321 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7381 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7441 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7501 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7561 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7621 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7681 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7741 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7801 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7861 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct
     7921 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7981 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8041 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8101 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8161 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8221 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8281 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8341 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8401 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8461 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8521 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8581 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8641 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8701 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     8761 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8821 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8881 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8941 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9001 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9061 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9121 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9181 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9241 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9301 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9361 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9421 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9481 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9541 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9601 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9661 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9721 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9781 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9841 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9901 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9961 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10021 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    10081 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    10141 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    10201 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    10261 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    10321 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    10381 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    10441 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    10501 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
