LOCUS       V042024                11619 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042024
VERSION     V042024
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5638
                     /label="RET(NM_001406743)"
                     /note="RET(NM_001406743)"
                     /gene="RET"
     CDS             5645..5734
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5778..5989
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6002..6270
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6299..6308
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6329..6991
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7061..7114
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7115..7714
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7715..8302
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8376..8609
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8681..8802
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8821..8956
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8994..9010)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9018..9034
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9042..9072)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9087..9108
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9396..9984)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10155..11015)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11016..11120)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11594..11610
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg cgaaggcgac gtccggtgcc gcggggctgc gtctgctgtt
     2341 gctgctgctg ctgccgctgc taggcaaagt ggcattgggc ctctacttct cgagggatgc
     2401 ttactgggag aagctgtatg tggaccaggc agccggcacg cccttgctgt acgtccatgc
     2461 cctgcgggac gcccctgagg aggtgcccag cttccgcctg ggccagcatc tctacggcac
     2521 gtaccgcaca cggctgcatg agaacaactg gatctgcatc caggaggaca ccggcctcct
     2581 ctaccttaac cggagcctgg accatagctc ctgggagaag ctcagtgtcc gcaaccgcgg
     2641 ctttcccctg ctcaccgtct acctcaaggt cttcctgtca cccacatccc ttcgtgaggg
     2701 cgagtgccag tggccaggct gtgcccgcgt atacttctcc ttcttcaaca cctcctttcc
     2761 agcctgcagc tccctcaagc cccgggagct ctgcttccca gagacaaggc cctccttccg
     2821 cattcgggag aaccgacccc caggcacctt ccaccagttc cgcctgctgc ctgtgcagtt
     2881 cttgtgcccc aacatcagcg tggcctacag gctcctggag ggtgagggtc tgcccttccg
     2941 ctgcgccccg gacagcctgg aggtgagcac gcgctgggcc ctggaccgcg agcagcggga
     3001 gaagtacgag ctggtggccg tgtgcaccgt gcacgccggc gcgcgcgagg aggtggtgat
     3061 ggtgcccttc ccggtgaccg tgtacgacga ggacgactcg gcgcccacct tccccgcggg
     3121 cgtcgacacc gccagcgccg tggtggagtt caagcggaag gaggacaccg tggtggccac
     3181 gctgcgtgtc ttcgatgcag acgtggtacc tgcatcaggg gagctggtga ggcggtacac
     3241 aagcacgctg ctccccgggg acacctgggc ccagcagacc ttccgggtgg aacactggcc
     3301 caacgagacc tcggtccagg ccaacggcag cttcgtgcgg gcgaccgtac atgactatag
     3361 gctggttctc aaccggaacc tctccatctc ggagaaccgc accatgcagc tggcggtgct
     3421 ggtcaatgac tcagacttcc agggcccagg agcgggcgtc ctcttgctcc acttcaacgt
     3481 gtcggtgctg ccggtcagcc tgcacctgcc cagtacctac tccctctccg tgagcaggag
     3541 ggctcgccga tttgcccaga tcgggaaagt ctgtgtggaa aactgccagg cattcagtgg
     3601 catcaacgtc cagtacaagc tgcattcctc tggtgccaac tgcagcacgc taggggtggt
     3661 cacctcagcc gaggacacct cggggatcct gtttgtgaat gacaccaagg ccctgcggcg
     3721 gcccaagtgt gccgaacttc actacatggt ggtggccacc gaccagcaga cctctaggca
     3781 ggcccaggcc cagctgcttg taacagtgga ggggtcatat gtggccgagg aggcgggctg
     3841 ccccctgtcc tgtgcagtca gcaagagacg gctggagtgt gaggagtgtg gcggcctggg
     3901 ctccccaaca ggcaggtgtg agtggaggca aggagatggc aaagggatca ccaggaactt
     3961 ctccacctgc tctcccagca ccaagacctg ccccgacggc cactgcgatg ttgtggagac
     4021 ccaagacatc aacatttgcc ctcaggactg cctccggggc agcattgttg ggggacacga
     4081 gcctggggag ccccggggga ttaaagctgg ctatggcacc tgcaactgct tccctgagga
     4141 ggagaagtgc ttctgcgagc ccgaagacat ccaggatcca ctgtgcgacg agctgtgccg
     4201 cacggtgatc gcagccgctg tcctcttctc cttcatcgtc tcggtgctgc tgtctgcctt
     4261 ctgcatccac tgctaccaca agtttgccca caagccaccc atctcctcag ctgagatgac
     4321 cttccggagg cccgcccagg ccttcccggt cagctactcc tcttccggtg cccgccggcc
     4381 ctcgctggac tccatggaga accaggtctc cgtggatgcc ttcaagatcc tggaggatcc
     4441 aaagtgggaa ttccctcgga agaacttggt tcttggaaaa actctaggag aaggcgaatt
     4501 tggaaaagtg gtcaaggcaa cggccttcca tctgaaaggc agagcagggt acaccacggt
     4561 ggccgtgaag atgctgaaag agaacgcctc cccgagtgag ctgcgagacc tgctgtcaga
     4621 gttcaacgtc ctgaagcagg tcaaccaccc acatgtcatc aaattgtatg gggcctgcag
     4681 ccaggatggc ccgctcctcc tcatcgtgga gtacgccaaa tacggctccc tgcggggctt
     4741 cctccgcgag agccgcaaag tggggcctgg ctacctgggc agtggaggca gccgcaactc
     4801 cagctccctg gaccacccgg atgagcgggc cctcaccatg ggcgacctca tctcatttgc
     4861 ctggcagatc tcacagggga tgcagtatct ggccgagatg aagctcgttc atcgggactt
     4921 ggcagccaga aacatcctgg tagctgaggg gcggaagatg aagatttcgg atttcggctt
     4981 gtcccgagat gtttatgaag aggattccta cgtgaagagg agccagggtc ggattccagt
     5041 taaatggatg gcaattgaat ccctttttga tcatatctac accacgcaaa gtgatgtatg
     5101 gtcttttggt gtcctgctgt gggagatcgt gaccctaggg ggaaacccct atcctgggat
     5161 tcctcctgag cggctcttca accttctgaa gaccggccac cggatggaga ggccagacaa
     5221 ctgcagcgag gagatgtacc gcctgatgct gcaatgctgg aagcaggagc cggacaaaag
     5281 gccggtgttt gcggacatca gcaaagacct ggagaagatg atggttaaga ggagagacta
     5341 cttggacctt gcggcgtcca ctccatctga ctccctgatt tatgacgacg gcctctcaga
     5401 ggaggagaca ccgctggtgg actgtaataa tgcccccctc cctcgagccc tcccttccac
     5461 atggattgaa aacaaactct atggcatgtc agacccgaac tggcctggag agagtcctgt
     5521 accactcacg agagctgatg gcactaacac tgggtttcca agatatccaa atgatagtgt
     5581 atatgctaac tggatgcttt caccctcagc ggcaaaatta atggacacgt ttgatagtgg
     5641 atccgaacaa aaactaatat cagaggaaga tttggaacag aaactaatca gtgaagaaga
     5701 tttagagcaa aagttaattt ccgaggagga cttatagcgg ccgcgaagga tctgcgatcg
     5761 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     5821 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     5881 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     5941 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
     6001 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
     6061 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
     6121 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
     6181 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
     6241 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc
     6301 caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg
     6361 caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg
     6421 ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct
     6481 gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa
     6541 ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga
     6601 ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg
     6661 cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat
     6721 catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg
     6781 cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag
     6841 ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt
     6901 cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca
     6961 gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc
     7021 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct
     7081 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg
     7141 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     7201 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     7261 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     7321 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     7381 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7441 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     7501 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     7561 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     7621 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     7681 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg
     7741 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7801 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7861 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7921 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7981 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     8041 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     8101 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     8161 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8221 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat
     8281 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     8341 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     8401 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     8461 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8521 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8581 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     8641 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     8701 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8761 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     8821 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8881 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8941 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag
     9001 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9061 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9121 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9181 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9241 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9301 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9361 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9421 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9481 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9541 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9601 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9661 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9721 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9781 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     9841 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9901 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9961 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10021 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10081 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10141 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10201 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10261 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10321 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10381 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10441 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10501 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10561 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10621 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10681 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10741 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10801 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10861 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10921 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10981 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11041 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11101 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    11161 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    11221 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    11281 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    11341 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    11401 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    11461 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    11521 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    11581 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
