LOCUS V026971 8664 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026971 VERSION V026971 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2301..3518 /label="RBMS1(NM_016836)" /note="RBMS1(NM_016836)" /gene="RBMS1" CDS 3525..3590 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3634..3845 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3858..4126 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4153..4752 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4759..5347 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5421..5654 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5726..5847 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5866..6001 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6039..6055) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6063..6079 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6087..6117) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6132..6153 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6441..7029) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7200..8060) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8061..8165) /gene="bla" /label="AmpR promoter" primer_bind 8639..8655 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta 2281 gagctagcga attcgccacc atgggcaaag tgtggaaaca gcagatgtac cctcagtacg 2341 ccacctacta ttacccccag tatctgcaag ccaagcagtc tctggtccca gcccacccca 2401 tggcccctcc cagtcccagc accaccagca gtaataacaa cagtagcagc agtagcaact 2461 caggatggga tcagctcagc aaaacgaacc tctatatccg aggactgcct ccccacacca 2521 ccgaccagga cctggtgaag ctctgtcaac catatgggaa aatagtctcc acaaaggcaa 2581 ttttggataa gacaacgaac aaatgcaaag gttatggttt tgtcgacttt gacagccctg 2641 cagcagctca aaaagctgtg tctgccctga aggccagtgg ggttcaagct caaatggcaa 2701 agcaacagga acaagatcct accaacctct acatttctaa tttgccactc tccatggatg 2761 agcaagaact agaaaatatg ctcaaaccat ttggacaagt tatttctaca aggatactac 2821 gtgattccag tggtacaagt cgtggtgttg gctttgctag gatggaatca acagaaaaat 2881 gtgaagctgt tattggtcat tttaatggaa aatttattaa gacaccacca ggagtttctg 2941 cccccacaga acctttattg tgtaagtttg ctgatggagg acagaaaaag agacagaacc 3001 caaacaaata catccctaat ggaagaccat ggcatagaga aggagaggtg agacttgctg 3061 gaatgacact tacttacgac ccaactacag ctgctataca gaacggattt tatccttcac 3121 catacagtat tgctacaaac cgaatgatca ctcaaacttc tattacaccc tatattgcat 3181 ctcctgtatc tgcctaccag gtgcaaagtc cttcgtggat gcaacctcaa ccatatattc 3241 tacagcaccc tggtgccgtg ttaactccct caatggagca caccatgtca ctacagcccg 3301 catcaatgat cagccctctg gcccagcaga tgagtcatct gtcactaggc agcaccggaa 3361 catacatgcc tgcaacgtca gctatgcaag gagcctactt gccacagtat gcacatatgc 3421 agacgacagc ggttcctgtt gaggaggcaa gtggtcaaca gcaggtggct gtcgagacgt 3481 ctaatgacca ttctccatat acctttcaac ctaataaggg atccgactac aaagaccatg 3541 acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag tagcggccgc 3601 gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc 3661 ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg 3721 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac 3781 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa 3841 cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag 3901 gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa 3961 ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc 4021 tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc 4081 aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg 4141 cgcctacgct agatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 4201 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 4261 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 4321 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 4381 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 4441 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 4501 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 4561 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 4621 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 4681 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 4741 cccggtgcct gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 4801 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 4861 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 4921 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 4981 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 5041 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 5101 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg 5161 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 5221 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 5281 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 5341 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 5401 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct 5461 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 5521 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 5581 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 5641 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 5701 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 5761 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 5821 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact 5881 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 5941 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 6001 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga 6061 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 6121 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 6181 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 6241 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 6301 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 6361 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 6421 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 6481 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 6541 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 6601 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 6661 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 6721 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 6781 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 6841 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 6901 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 6961 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 7021 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 7081 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 7141 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 7201 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 7261 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 7321 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 7381 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 7441 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 7501 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 7561 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 7621 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 7681 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 7741 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 7801 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 7861 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 7921 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 7981 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 8041 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 8101 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 8161 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 8221 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac 8281 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 8341 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg 8401 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 8461 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc 8521 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg 8581 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 8641 aaaacgacgg ccagtgccaa gctg //