LOCUS       V020853                11202 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020853
VERSION     V020853
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5287
                     /label="RBM10(NM_001204468)"
                     /note="RBM10(NM_001204468)"
                     /gene="RBM10"
     CDS             5288..5311
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     promoter        5361..5572
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5585..5853
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5882..5891
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5912..6574
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6644..6697
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6698..7297
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7298..7885
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7959..8192
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8264..8385
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8404..8539
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8577..8593)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8601..8617
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8625..8655)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8670..8691
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8979..9567)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9738..10598)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10599..10703)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11177..11193
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgtctgg gagcccttcc ttgacagccc gggccgagaa
     2341 ggtgagcgtc gacgctggtc gtgggggcgg agagtccctg caggaagcat cacccaggct
     2401 ggcagatcat ggtagcagca gcgggggtgg ctgggaagtg aaacggagcc agcggctgag
     2461 gaggggcccc agcagccccc gaaggcccta tcaggacatg gagtatgaaa gacgtggtgg
     2521 tcgtggtgac aggactggcc gctatggagc cactgaccgc tcgcaggatg atggtgggga
     2581 gaaccgcagc cgagaccacg actaccggga catggactac cgttcatatc ctcgcgagta
     2641 tggcagccag gagggcaagc atgactatga cgactcatct gaggagcaga gtgcggagga
     2701 ttcctacgag gcctccccgg gctccgagac tcagcgtagg cggcggcggc ggcacaggca
     2761 cagccccacc ggcccgccag gcttcccccg agacggcgac tatcgggacc aggactatcg
     2821 gaccgagcaa ggggaggagg aggaggagga ggaggatgag gaggaggagg agaaggccag
     2881 taacatcgtc atgctgagga tgctgccaca ggcagccact gaggatgaca tccgtggcca
     2941 gctgcagtcg cacggcgtgc aagcacggga ggttcggctg atgcggaaca aatcttcagg
     3001 tcagagccgg ggcttcgcct tcgtcgagtt tagtcacttg caggacgcta cacgatggat
     3061 ggaagccaat cagcactccc tcaacatcct gggccagaag gtgtcgatgc actacagtga
     3121 ccccaagccc aagatcaatg aggactggct gtgcaataag tgtggcgtcc agaacttcaa
     3181 acgccgagag aagtgcttca aatgtggcgt gcccaagtca gaggcagagc agaagctgcc
     3241 cctcggcacg aggctggatc agcagacact gccactgggt ggccgggagc tgagccaggg
     3301 cctgcttccc ctgccgcagc cctaccaggc ccagggagtc ctggcctccc aagccctgtc
     3361 acagggctcg gagccaagct cagagaacgc caatgacacc atcattttgc gcaacctgaa
     3421 cccacacagc accatggatt ccatcctggg ggccctggca ccctacgcgg tgctgtcctc
     3481 ctccaacgtg cgcgtcataa aggacaagca gacccaactg aaccgcggct ttgccttcat
     3541 ccagctctcc accatcgtgg aggcagccca gctgctgcag atcctgcagg ccctgcaccc
     3601 accactcact atcgacggca agaccatcaa tgttgagttt gccaagggtt ctaagaggga
     3661 catggcctcc aatgaaggca gtcgcatcag tgctgcctct gtggccagca ctgccattgc
     3721 tgcggcccag tgggccatct cacaggcctc ccaaggtggg gagggtacct gggccacctc
     3781 cgaggagccg ccggtcgact acagctacta ccaacaggat gagggctatg gcaacagcca
     3841 gggcacagag tcttccctct atgcccatgg ctacctcaag ggcaccaagg gccctggcat
     3901 cactggaacc aaaggggatc ccactggagc aggtcccgag gcctccctag agcctggggc
     3961 cgactctgtg tcgatgcagg ctttctctcg cgcccagcct ggtgctgctc ctggcatcta
     4021 ccaacaatca gccgaggcga gcagtagcca gggcactgct gccaacagcc agtcgtatac
     4081 catcatgtca cccgctgtgc tcaaatctga gctccagagc cctacccatc ctagttctgc
     4141 tctcccaccg gctaccagcc ccactgccca ggaatcctac agccagtacc ctgttcccga
     4201 cgtctctacc taccagtacg atgagacctc cggctactac tatgaccccc agaccggcct
     4261 ctactatgac cccaactccc agtattacta caatgctcag agccagcagt acctgtactg
     4321 ggatggggag aggcggacct atgttcccgc cctggagcag tcggccgacg gacataagga
     4381 gacaggggca ccctcgaagg agggcaaaga gaagaaggag aagcacaaga ccaagacagc
     4441 tcaacagatt gccaaggaca tggaacgctg ggcccgcagt ctcaacaaac aaaaagaaaa
     4501 cttcaaaaat agcttccagc ctatcagctc cctgcgagat gacgagaggc gggagtcagc
     4561 cactgcagat gctggctatg ccatcctcga gaagaaggga gcactagccg agagacagca
     4621 caccagcatg gatctcccga aattggccag tgacgaccgc ccaagccctc cgcgaggact
     4681 ggtggcagcc tacagcgggg agagtgacag tgaggaggag caggagcgtg ggggccctga
     4741 gcgggaggag aagctcaccg actggcagaa gctggcctgt ctgctctgcc gacgccagtt
     4801 ccccagcaaa gaggcgctca tccggcacca gcagctctca gggctccaca agcaaaacct
     4861 tgagattcac cggcgagccc acttgtcaga aaacgagcta gaagcactag agaagaatga
     4921 catggagcaa atgaagtacc gggaccgtgc agctgaacgc agagaaaagt atggcatccc
     4981 cgagccgcca gagcccaaga ggaggaagta cggcggcata tccacagcct ctgtagactt
     5041 cgagcagcct actcgggacg ggctgggcag tgacaacatt ggcagtcgga tgctgcaggc
     5101 catgggctgg aaagagggca gcggcctggg ccgcaagaag cagggcattg taacgcctat
     5161 cgaggcccaa acacgggtgc ggggctccgg cctgggtgca cggggcagct cctacggggt
     5221 cacctcaacc gagtcctaca aggagacact gcacaagaca atggtgaccc gcttcaacga
     5281 ggcccaggat tacaaggacg acgatgacaa gtaggatccg cggccgcgaa ggatctgcga
     5341 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     5401 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     5461 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     5521 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     5581 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     5641 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     5701 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     5761 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     5821 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga
     5881 cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac
     5941 cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca
     6001 gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct
     6061 gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga
     6121 gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta
     6181 cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat
     6241 cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa
     6301 gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt
     6361 gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga
     6421 cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat
     6481 gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta
     6541 ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc
     6601 tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct
     6661 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt
     6721 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6781 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     6841 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6901 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6961 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     7021 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     7081 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     7141 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     7201 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     7261 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat
     7321 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7381 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7441 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7501 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7561 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7621 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7681 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     7741 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7801 cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg
     7861 gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     7921 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7981 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     8041 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8101 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8161 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     8221 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     8281 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8341 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     8401 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8461 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8521 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca
     8581 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8641 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8701 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8761 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8821 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8881 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8941 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9001 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9061 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9121 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9181 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9241 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9301 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9361 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     9421 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9481 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9541 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9601 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9661 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9721 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9781 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9841 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9901 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9961 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10021 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10081 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10141 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10201 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10261 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10321 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10381 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10441 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10501 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10561 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10621 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10681 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    10741 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    10801 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    10861 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    10921 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    10981 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
    11041 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    11101 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    11161 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
