LOCUS       V037672                11872 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037672
VERSION     V037672
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6726
                     /label="Pxdn(NM_181395)"
                     /note="Pxdn(NM_181395)"
                     /gene="Pxdn"
     CDS             6733..6798
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6842..7053
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7066..7334
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             7361..7960
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7967..8555
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8629..8862
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8934..9055
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9074..9209
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9247..9263)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9271..9287
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9295..9325)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9340..9361
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9649..10237)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10408..11268)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11269..11373)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11847..11863
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggccgtg cgccccacgc gccgctgcct gctggcgctc
     2341 ctgctgtgct ttgcctggtg ggccatggcg gtggtcgcct cgaagcaagg ggcaggctgt
     2401 ccaagccgct gcctgtgttt ccgtaccacc gtgcgctgca tgcatctgtt gctggaggcc
     2461 gtgcccgccg tggcgccgca gacctccatc ctagatcttc ggttcaacag aatcagagag
     2521 atccaacccg gggcattcag gaggctgagg agcctgaaca cactgcttct taacaacaac
     2581 cagatcaaga agatccccaa tggtgcattt gaggacctgg agaacttaaa atacctctat
     2641 ttgtacaaga atgagatcca atcaattgac aggcaagcat ttaagggact tgcctctcta
     2701 gagcaactgt acctgcactt taatcagata gaaacgctgg accctgaatc cttccagcac
     2761 ctgccaaagc tggagagact gtttttgcac aacaaccgta tcacgcactt agttcctggg
     2821 acgttcagtc agttagagtc catgaaacgg ctgcgattgg actcgaatgc actccactgt
     2881 gactgtgaaa tcctatggct agcggatcta ctgaagacct acgcccaatc tggaaacgca
     2941 caagcagcag ctacatgcga gtatcccaga cgcatccaag gacgctctgt ggctaccatc
     3001 accccagaag agctgaactg tgaaaggccc cggattacct cagagccaca ggatgcagat
     3061 gtcacctcag ggaacacagt gtacttcacc tgcagagctg agggcaaccc caaacctgag
     3121 atcatctggc ttcgaaacaa taacgagttg agcatgaaga cggactctcg cttaaacttg
     3181 ctggacgatg gcacgctgat gattcagaac acacaggagg cggatgaggg tgtctaccag
     3241 tgcatggcga aaaatgtggc tggagaggcg aaaacgcagg aggtgaccct caggtacttg
     3301 gggtctccag cccgacccac ttttgtaatc cagccgcaga acacagaggt actggtgggt
     3361 gagagtgtca ctctggagtg cagtgccaca ggccaccctc tgcctcagat cacctggaca
     3421 agaggtgacc gcacaccctt gccaattgac cctcgagtga atatcactcc ctctggagga
     3481 ctgtatatac agaacgtcgc ccagagtgac agcggcgagt acacgtgctt tgcatccaat
     3541 agtgtggaca gtatccatgc cacagccttc atcattgtac aagcccttcc tcagttcact
     3601 gtgaccccac agagccgagt ggtcattgaa gggcagactg tggatttcca gtgtgcggct
     3661 aagggacacc ctcagcctgt catagcctgg accaagggag ggagccagct ctcagtggac
     3721 aggcggcacc tggtgctgtc ctcaggaaca ctcaggattt ctggggtggc cctgcatgac
     3781 cagggccagt atgagtgcca ggccgtcaat atcattggct cccagaaggt cgtggcccac
     3841 ctgacagtac agcctagagt caccccggta tttgccagca ttcccagtga catgactgta
     3901 gaggtgggca ccaacgtgca gctgccttgt agctcccagg gagaaccaga gccagccatc
     3961 acctggaaca aggatggtgt tcaggtaaca gaaagtggaa aatttcacat cagccccgaa
     4021 ggattcttga ccatcaatga tgttggcact gccgatgcag gtcgctatga gtgtgtagct
     4081 cggaacacaa ttggatatgc ctctgtgagc atggtactca gtgtgaatgt tcctgatgta
     4141 agtcggaatg gggatcccta tgttgctacc tctattgttg aagccattgc aactgttgac
     4201 agagccatca actctacaag gacacacttg tttgacagcc gtcctcgttc tccaaatgac
     4261 ctgctcgctc tgttccggta cccacgggat ccatacacag tgggacaagc cagggcagga
     4321 gagatattcg agcggaccct gcagctgatc caggagcatg ttcagcatgg cttgatggtg
     4381 gacttgaatg gaacaagtta ccactacaat gatctggtgt ccccgcagta cctgagcctc
     4441 atcgccaacc tgtcaggctg cactgcacac cgccgcgtga acaactgctc agacatgtgc
     4501 ttccaccaga agtataggac gcacgatggc acgtgcaaca atctacagca cccgatgtgg
     4561 ggtgcctcac tgaccgcctt tgagcgcctg ttgaaggctg tgtatgagaa tgggttcaac
     4621 acaccccggg gcattaattc ccagcgtcag tacaatgggc atgtactacc catgccccgc
     4681 ctggtgtcca ccacactgat tgggacagag gtgatcaccc ccgatgagca gtttacacac
     4741 atgctgatgc aatggggcca gttccttgac catgacctag actctacagt ggtagccctg
     4801 agccaggccc gcttctctga cggccagcat tgcagctctg tgtgcagcaa tgaccctccc
     4861 tgtttctcgg tcatgatccc ccccaatgat ccccgggtgc ggagtggcgc ccgatgcatg
     4921 ttcttcgtgc gatcgagccc cgtgtgtggc agcggcatga cgtccctgct catgaactct
     4981 gtgtaccctc gagagcagat caaccagctc acctcctaca tcgatgcctc caatgtgtac
     5041 ggcagcacag accacgaagc ccgcagcatc cgggacctgg ccagccaccg tggcctgctg
     5101 cgtcagggca ttgtgcagag gtctggcaag cccctgcttc cctttgccac cgggccaccc
     5161 actgagtgca tgcgcgatga gaacgagagc ccgataccat gctttctggc cggcgaccac
     5221 cgtgccaacg agcagcttgg cctgaccagc atgcatacgc tgtggttccg ggagcacaac
     5281 cgcattgcag cagagctgtt gaagctaaac ccgcactggg atggggacac tgtctaccat
     5341 gagacccgca agatagtcgg ggcagagata cagcacatca cctaccggca ctggctgccc
     5401 aagatcctgg gggaggtggg catgaagatg ctcggtgagt accggggcta cgaccccagt
     5461 gtcaatgctg gcatctttaa tgcctttgcc actgcagcct tcaggttcgg tcacactctg
     5521 atcaaccctc tgctctaccg gctggatgag aactttgagc ccatccctca gggccatgtg
     5581 cccctccaca aagccttctt ctcgcccttc cggattgtca acgagggggg catcgaccca
     5641 cttctccgag ggctgtttgg agtggcaggg aagatgcgca ttccctctca attgctgaac
     5701 acagagctca cggagaggct gttctccatg gcccacacag tggccctgga cctggctgcc
     5761 atcaatatcc agcgaggccg ggaccatggc atcccaccct accatgacta cagagtctac
     5821 tgcaacttgt cggctgctta cacctttgag gacctgaaaa atgagatcaa gagccctgtg
     5881 atccgggaga aactgcagag gctgtatggc tcgactctca acattgatct gttcccagcc
     5941 ctcatggtag aagacctagt acctggcagc cgcttggggc ccacactcat gtgcctgctc
     6001 agcacacagt tccgacgcct gcgggatgga gacaggttgt ggtatgagaa cccaggcgtg
     6061 ttctcccccg cccagctgac tcagctcaag cagacgtccc tggcgaggat cctttgtgac
     6121 aactcagaca acatcacccg tgtgcagcag gatgtgttca gggtggcaga gttcccccac
     6181 ggttatagca gctgtgagga catccccagg gtggacctgc gagtgtggca ggactgttgt
     6241 gaagattgta ggaccagggg acaattcaat gctttctcct accatttccg gggaagacgg
     6301 tctctagaat ttagctatga ggacgataag cccacaaaga gagccaggtg gcggaaagca
     6361 ctaagtgtaa agcatggcaa acatcttagc aatgccacat cagccaccca cgagcacttg
     6421 gaagggccag caactaatga tctcaaggaa tttgttctgg aaatgcaaaa gatcatcaca
     6481 gacctcagaa aacagataaa cagcttggag tctcggctca gcaccacaga atgtgtggat
     6541 gacagcggtg aatctcacgg cggcaacaca aagtggaaaa aagacccatg cacagtttgt
     6601 gagtgcaaaa atggccagat cacctgcttt gtggaagctt gccagcctgc agcctgcccc
     6661 cagcctgtga aagtggaagg cgcttgctgt cccgtctgct taaagaacac tgcagaggaa
     6721 aagcctggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     6781 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     6841 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6901 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6961 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     7021 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     7081 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     7141 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     7201 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     7261 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     7321 cgccgttaca gatccaagct gtgaccggcg cctacgctag atgaccgagt acaagcccac
     7381 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     7441 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     7501 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     7561 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     7621 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     7681 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     7741 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     7801 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     7861 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     7921 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc aacctctgga
     7981 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     8041 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     8101 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     8161 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     8221 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     8281 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     8341 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     8401 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     8461 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     8521 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt
     8581 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8641 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8701 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8761 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8821 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     8881 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     8941 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9001 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9061 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9121 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9181 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta
     9241 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9301 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9361 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9421 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9481 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9541 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9601 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9661 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9721 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9781 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9841 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9901 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9961 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10021 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10081 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10141 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10201 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10261 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10321 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10381 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10441 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10501 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10561 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10621 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10681 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10741 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10801 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10861 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10921 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10981 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11041 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11101 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11161 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11221 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11281 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11341 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11401 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    11461 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    11521 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    11581 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    11641 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    11701 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    11761 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    11821 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
