LOCUS       V025655                14455 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025655
VERSION     V025655
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    113..134
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        149..179
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    187..203
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     211..227
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        248..266
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        294..520
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             521..701
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    748..873
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1366..1599
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1784..1828
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2095..2211
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     enhancer        2234..2537
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2538..2741
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..2844
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     CDS             2845..8781
                     /label="TRIP11(NM_004239)"
                     /note="TRIP11(NM_004239)"
                     /gene="TRIP11"
     CDS             8818..9537
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        9587..9916
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9926..10525
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10592..11180
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11267..11500
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11572..11693
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11733..11868
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(11889..11907)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(11917..11933)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      12075..12530
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        12556..12660
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             12661..13521
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      13692..14280
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
       61 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      241 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
      301 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
      361 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
      421 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
      481 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
      541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      721 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      781 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      901 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      961 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1021 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1081 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1141 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1201 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1261 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1321 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1381 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1441 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1501 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1561 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1621 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1681 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1741 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1801 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1861 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1921 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     1981 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2041 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     2101 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2161 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
     2221 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2401 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2461 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2521 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2641 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     2761 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagagcc
     2821 accatgcacc atcaccatca ccatatgtcg tcctggcttg ggggcctcgg ctccggattg
     2881 ggccagtctc tgggtcaagt cgggggcagc ctggcttccc tcactggcca gatatcaaac
     2941 tttacaaagg atatgctgat ggagggcacg gaggaagtgg aagcagaatt acctgattct
     3001 aggacaaagg aaattgaagc cattcatgca atcttgagat cagagaatga aaggcttaag
     3061 aaactttgta ctgatctaga agagaaacat gaagcatcag agattcaaat aaagcagcaa
     3121 tctacaagtt accgaaatca acttcaacaa aaagaggtag aaatcagcca tcttaaagcc
     3181 agacagattg cactccagga tcagttgctg aaactgcagt cagctgctca gtcagtacct
     3241 tcaggagctg gtgtaccagc aaccactgca tcatcttcat tcgcttatgg gattagtcat
     3301 catccttcag ctttccatga cgatgacatg gactttggtg atataatttc atcccaacaa
     3361 gaaataaacc gactctcaaa tgaagtttca agacttgagt ctgaagttgg ccattggagg
     3421 catattgctc agacttccaa agcacaagga acagataact ctgatcaaag tgaaatatgt
     3481 aaactacaaa atatcattaa ggaactaaaa cagaaccgaa gtcaggaaat tgatgaccat
     3541 caacatgaaa tgtcagtact gcagaatgca caccaacaga aattgacaga aataagtcga
     3601 cgacatcgag aagaattaag tgactatgaa gaacgaattg aagaacttga aaatctgtta
     3661 caacaaggtg gctctggagt tatagaaact gatctctcta aaatctatga gatgcaaaaa
     3721 actattcaag ttctacaaat agaaaaagtg gagtctacca aaaaaatgga acaacttgag
     3781 gataaaataa aagatataaa taaaaaatta tcttctgcag aaaatgacag agatattttg
     3841 aggagagaac aagaacagct aaatgtggaa aagagacaaa taatggaaga atgtgaaaac
     3901 ttgaaattgg aatgtagtaa attgcagcct tctgctgtga agcaaagtga tactatgaca
     3961 gaaaaggaaa gaattcttgc ccagagtgca tcagtggaag aagtgttcag actacaacaa
     4021 gcactgtctg atgccgaaaa tgaaataatg agattgagta gtttaaacca ggataacagt
     4081 cttgctgaag acaatctgaa acttaaaatg cgtatcgaag ttttagaaaa agagaagtca
     4141 ttactgagtc aagaaaagga agaacttcag atgtcacttt taaaattgaa caatgaatat
     4201 gaagtaatta aaagtacagc tacaagagac ataagtttgg attcagaatt acatgactta
     4261 agacttaatt tggaggcaaa ggaacaagaa ctcaatcaga gtattagtga aaaggaaaca
     4321 ctgatagctg agatagaaga attggacaga cagaatcaag aagctacaaa gcacatgatt
     4381 ttgataaaag atcagctatc aaaacaacaa aatgaaggag atagcatcat cagtaaactg
     4441 aaacaagatc taaatgatga aaaaaagaga gttcatcaac ttgaagatga taaaatggac
     4501 attactaaag agttagatgt acagaaagaa aagctaattc aaagtgaagt ggccctaaat
     4561 gatttacatt taaccaagca gaaacttgag gacaaagtag aaaatttagt agatcagcta
     4621 aataaatcac aagaaagtaa tgtaagcatc cagaaggaga atttagaact taaggagcat
     4681 attagacaaa atgaggagga gctttctaga ataaggaatg agttaatgca gtctctaaat
     4741 caagactcta atagtaattt taaggatacc ttacttaaag aaagagaagc tgaagttaga
     4801 aacttaaagc aaaatctttc agaattagaa cagctcaatg aaaatttaaa gaaagttgct
     4861 tttgatgtca aaatggaaaa tgaaaagtta gttttagcat gtgaagatgt gaggcatcag
     4921 ttagaagaat gtcttgctgg taacaatcag ctttctctgg aaaaaaacac tattgtggag
     4981 actctaaaaa tggaaaaagg agagatagag gcagaattgt gttgggctaa aaagaggctg
     5041 ttggaagaag caaacaagta tgagaaaacc attgaagaac tgtcaaatgc acgtaatttg
     5101 aatacctctg ccttacagct ggaacatgag catttaatta aactcaatca aaagaaagac
     5161 atggaaatag cagaactcaa aaagaatatt gaacaaatgg atactgacca taaagaaact
     5221 aaggacgttt tgtcatctag tttagaagag cagaagcagt tgacacaact tataaacaag
     5281 aaagaaattt ttattgaaaa gcttaaagaa agaagttcaa agctgcagga ggaattggat
     5341 aaatattctc aggccttaag aaaaaatgaa attttaagac agaccataga ggaaaaagac
     5401 cgaagtcttg gatccatgaa agaggaaaat aatcatctgc aagaagaatt ggaacgactc
     5461 agggaagagc agagtcgaac cgcacctgtg gctgacccta aaacccttga tagtgttact
     5521 gaactagcat ctgaggtatc tcaactgaac acgatcaagg aacatcttga agaggaaatt
     5581 aaacatcatc aaaagataat tgaagatcaa aaccagagta agatgcaact acttcagtct
     5641 ttacaagagc aaaagaagga aatggatgag tttagatacc agcatgagca aatgaacgcc
     5701 acacacaccc agctcttttt agagaaggat gaggaaatta agagtttgca aaaaacaatt
     5761 gaacaaatca aaacccagtt gcatgaagaa agacaggaca ttcaaacaga taactctgat
     5821 atttttcaag aaacaaaagt tcagagcctt aatatagaaa atggaagtga aaagcatgat
     5881 ttatctaaag ctgaaacgga aagattagtg aaaggaataa aagagcgaga actggagatt
     5941 aaacttctaa atgaaaagaa tatatcttta actaaacaga ttgatcagtt gtccaaagat
     6001 gaagttggta aactaactca gattattcag cagaaagatt tggagataca agctcttcat
     6061 gctagaattt cttcaacttc ccatactcaa gatgttgttt accttcaaca gcaactgcag
     6121 gcttatgcta tggaaagaga aaaggtattt gctgttttga atgagaagac tagggaaaat
     6181 agccatctaa aaacagaata tcacaaaatg atggatattg ttgctgccaa ggaagcagct
     6241 cttatcaaac tgcaagatga aaataaaaaa ttgtccacta gatttgaaag tagtggccaa
     6301 gatatgttta gagaaactat tcagaattta tcacgtatca ttcgagaaaa agacatcgaa
     6361 atagatgcac taagtcagaa atgtcagact ttattggcag ttttacaaac atccagcact
     6421 ggtaatgagg ctggaggtgt taatagtaat caatttgagg agcttctaca ggaacgtgac
     6481 aagttaaaac agcaagtaaa gaaaatggaa gagtggaagc agcaggtgat gaccacagta
     6541 caaaatatgc aacacgagtc agcccagctt caggaagagc ttcaccaact tcaagcacag
     6601 gttttggttg acagtgataa taattctaaa ttacaagtgg actatactgg cctgatccaa
     6661 agttatgagc agaatgaaac caaactcaaa aattttgggc aggaattagc acaagttcag
     6721 cacagcattg ggcagctttg caataccaag gatcttcttt taggaaaact tgatattatt
     6781 tcaccccagc tgtcttctgc atcattgctt actccccagt ctgcagagtg tcttagagca
     6841 agtaagtctg aagtattgag tgaatcttct gaattgcttc agcaagagtt agaagagcta
     6901 agaaaatcac tacaggaaaa agatgcaaca attagaactc tccaggaaaa taaccacaga
     6961 ttgtctgatt cgattgctgc cacctcagag ctagaaagaa aagaacacga acaaaccgat
     7021 tcagaaatca agcagctaaa ggagaaacaa gatgttttgc aaaagttact taaggaaaaa
     7081 gacctcttaa tcaaagccaa aagtgatcaa ctactttctt ccaatgaaaa tttcactaac
     7141 aaagtaaatg aaaacgaact tttgaggcag gcagtaacaa acctgaagga gagaatatta
     7201 attctagaga tggacattgg caaactaaaa ggagaaaatg aaaaaatagt ggaaacatac
     7261 aggggaaagg aaacagaata tcaagcgtta caagagacta acatgaagtt ttctatgatg
     7321 ctgcgagaaa aagagtttga gtgccactca atgaaggaga aggctcttgc ttttgaacag
     7381 ctattgaaag agaaagaaca gggcaagact ggagagttaa atcagctttt aaatgcagtt
     7441 aaatcaatgc aggagaagac agttgtgttt caacaggaga gagaccaagt catgttggcc
     7501 ctgaaacaaa aacaaatgga aaatactgcc ctacagaatg aggttcaacg tttacgtgac
     7561 aaagaatttc gttcaaacca agagctagag agattgcgta atcatctttt agaatcagaa
     7621 gattcttata cccgtgaagc tttggctgca gaagatagag aggctaaact aagaaagaaa
     7681 gtcacagtat tggaggaaaa gctagtttca tcctctaatg caatggaaaa tgcaagccat
     7741 caagccagtg tgcaggtaga gtcattgcaa gaacagttga atgtagtttc caagcaaagg
     7801 gatgaaactg cgctgcagct ttctgtctct caggaacaag taaagcagta tgctctgtca
     7861 ctggccaacc tgcagatggt actagagcat ttccaacaag aggaaaaagc tatgtattct
     7921 gctgaactcg aaaagcaaaa acagcttata gctgaatgga agaaaaacgc agaaaatctg
     7981 gaaggaaaag tgatatcatt acaggaatgt ttggatgaag caaatgctgc attggattca
     8041 gcatcaagac ttacagaaca gttagatgta aaagaagaac aaattgaaga acttaaaaga
     8101 caaaatgagc tccgacaaga aatgctggat gatgtacaaa agaaattgat gagcttagca
     8161 aacagctcag aaggaaaagt agacaaagtc ctaatgagaa acctcttcat tggtcatttc
     8221 cacacaccga aaaatcagcg tcatgaagtg ttacggttaa tggggagcat cctgggcgtc
     8281 agaagggagg agatggagca gttgtttcat gacgatcagg gcggtgttac caggtggatg
     8341 actgggtggc ttggaggagg atcaaaaagt gttcccaaca cacctttgag accaaatcag
     8401 caatctgtgg ttaatagttc tttttcagaa ctttttgtta aatttctaga aacagaatct
     8461 catccatcca ttccaccacc aaagctttct gttcatgata tgaaacctct ggattcacca
     8521 ggaagaagaa aaagagatac aaatgcacca gaaagtttta aagatacagc agaatccagg
     8581 tctggtagaa gaacagatgt aaatccgttt ttggctcctc gctcggcagc tgtacctctt
     8641 attaacccag ctggacttgg acctggtggg cccgggcatc ttcttctgaa acccatctca
     8701 gatgttttgc ccacatttac acctttgcca gcgttacctg acaacagtgc tggggttgtg
     8761 ctgaaagacc ttttaaagca actcgagggc ggtggaggca gtggtggagg cggtagcatg
     8821 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     8881 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     8941 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     9001 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     9061 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     9121 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     9181 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     9241 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     9301 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     9361 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     9421 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     9481 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaacgg
     9541 gatccggact agttaacgcg tcgaccagtg gtcgaccctg tggaatgtgt gtcagttagg
     9601 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     9661 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9721 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     9781 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     9841 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9901 cctaggcttt tgcaaaaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     9961 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
    10021 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
    10081 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
    10141 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
    10201 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
    10261 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
    10321 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
    10381 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
    10441 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
    10501 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
    10561 gagcgcacga ccccatgcat cgccagtcga caatcaacct ctggattaca aaatttgtga
    10621 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
    10681 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
    10741 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
    10801 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
    10861 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
    10921 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
    10981 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
    11041 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
    11101 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
    11161 cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc aatgacttac
    11221 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
    11281 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
    11341 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
    11401 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
    11461 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
    11521 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
    11581 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    11641 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    11701 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
    11761 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    11821 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
    11881 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
    11941 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
    12001 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
    12061 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
    12121 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
    12181 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
    12241 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
    12301 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
    12361 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
    12421 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
    12481 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
    12541 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
    12601 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
    12661 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
    12721 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
    12781 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
    12841 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
    12901 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
    12961 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
    13021 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
    13081 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
    13141 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
    13201 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
    13261 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    13321 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
    13381 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
    13441 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    13501 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
    13561 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    13621 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    13681 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    13741 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    13801 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    13861 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    13921 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    13981 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    14041 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    14101 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    14161 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    14221 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    14281 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    14341 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    14401 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaag
//
