LOCUS       V031859                11398 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031859
VERSION     V031859
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    113..134
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        149..179
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    187..203
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     211..227
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        248..266
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        294..520
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             521..701
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    748..873
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1366..1599
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1784..1828
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2095..2211
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     enhancer        2234..2537
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2538..2741
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2824..5652
                     /label="Ncoa7(NM_172495)"
                     /note="Ncoa7(NM_172495)"
                     /gene="Ncoa7"
     CDS             5659..5724
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             5761..6480
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        6530..6859
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6869..7468
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7535..8123
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8210..8443
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8515..8636
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8676..8811
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(8832..8850)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(8860..8876)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      9018..9473
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9499..9603
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             9604..10464
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      10635..11223
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
       61 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      241 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
      301 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
      361 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
      421 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
      481 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
      541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      721 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      781 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      901 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      961 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1021 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1081 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1141 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1201 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1261 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1321 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1381 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1441 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1501 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1561 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1621 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1681 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1741 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1801 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1861 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1921 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     1981 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2041 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     2101 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2161 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
     2221 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2401 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2461 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2521 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2641 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     2761 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagagcc
     2821 accatggata ctaaggaaga gaagaaggaa cagaaggaac ggaaacagag ttattttgct
     2881 agactaaaaa agaaaaaaca agccaaacaa aatgcagaga tagtttcagc tgcgtcctcg
     2941 aagagtcgtt ccgggaaaga tgatgctaat tcagacattt tggaacaaga caagttcaac
     3001 gttacagcag agggggacca tagcactgat gacaaaaaga agagaaaaag taaccagtta
     3061 aaggaaatca ggcgcacgga actgaagaga tactacagtg ttgatgacaa tcaaaacaaa
     3121 actcatgata aaaaagagaa gaagatgatg gtccaaaagc cacagggcac catggaatac
     3181 actgctggaa gccaggacac cctcaactct gttgcactga agtttaacgt cactcccaat
     3241 aaattagtgg aattgaataa actcttcaca cacactattg ttccaggcca ggtcctgttt
     3301 gtgccagatg ccaacatctc ttctagtacc atacagcttt cttcatccac ccctggtgct
     3361 actgtctctc catcatcatc agatgcagag tatgataagc tgcctgatgc agatttagca
     3421 agaaaggcct taaaacccat cgagagggtc ttatcatcta cttccgaaga agatgagcca
     3481 ggtgttgtaa aattcctcaa aatgaactgt cgctacttca ctgatggaaa gggtgtggtt
     3541 gggggtgtca tgattgtcac tcctaacaac atcatgtttg accctcataa gtccgatccc
     3601 ttagtgattg aaaacggttg cgaagaatat ggtctcatct gccccatgga agaggtggtc
     3661 tccattgccc tgtacagtga catatcccac atgaagatca aagacgcctt gccatctgac
     3721 ctacctcggg atctttgtcc tctgtacagg cctggagaat gggaggacct ggcttcagag
     3781 aaagatatca atccattcag taaattcaag tccatcaaca aggaaaaacg acagcagaat
     3841 ggggagagga cccttgcttt agatgccaaa tcagtgcgct ctcctgagga gtcaactgaa
     3901 cgtacttgca cacgcataga gcccccagac aactctgaat tgtggaaatt aaagaatctg
     3961 caatcttcca ggggaacaac cagtgagtct ccctcagtga cccagctcag catgcgtgct
     4021 gccttggaac ctacagccaa agaaaactgt cttttaaaag gagatgagga ctttgttgac
     4081 ttggaagaac tttcttctca acctgagagt ggaatcaaca aaggagatgc cacgaaagaa
     4141 tgtctttcct atgaccaaaa gaaggatggg ccgcatacag tcatgtctgc aaagaagggg
     4201 cacgtgcagt cagcccagga agttatggga ccagagagtg atactgaact gaagggagcc
     4261 ctagatctag aaactgctga gaagcaagat gaagccccag aagtagacaa acactctgga
     4321 tccccagaaa acttggggga aagcacgctg aatatacacg aagatctaga taaaattaaa
     4381 ctcattgaat tttacctaaa taaaaacaag gaagggtcac agctgttaga aaatgtgcag
     4441 aagtcagagt taagtgaccg caaaagtatt gaaccaggag gaatagacat cacacttagt
     4501 agctctcttc cccaagcagg tgattcccca cctgaggaca acaaagagcc aggtacactc
     4561 tgggtaaaag ggggagaaac tctgcccctg aaactagatc ctagtgcaga ggaaactgta
     4621 attaacaata aagaggttct ggattctttg ggatcatctc tagacaagat gtgtcacagt
     4681 gcacagatgg ataacaaatc tgagattcag ttatggctgt taaagagaat tcaggtacca
     4741 attgaagata tacttccctc aaaagaagag aagagtaaga ccccgcccat gtttctgtgc
     4801 atcaaagtgg gaaagccaat gagaaaatcc tttgccagtc acaccgccac catggttcag
     4861 cagtatagca agagaagaaa gcagcctgaa tactggtttg ctgttccccg ggagagggtg
     4921 gatcacttgt acaccttctt tgttcagtgg tctcctgatg tctatgggaa agatgccaag
     4981 gagcaaggct ttgtggtggt ggagaaggaa gagctgaaca tgatcgacaa tttcttcagt
     5041 gagccgacca ccaagagctg ggagatcatc acagttgagg aggcaaaacg caggaagagc
     5101 acttgcagct actacgagga agaggaagaa gaggaagagg gactgcctat cctacagccc
     5161 cacagtgcac tcctggaaaa catgcacatc gaacagctgg cccgacgact tccagccagg
     5221 gtacaaggct atccatggag actggcctac agcacattag agcatggaac gagcttgaag
     5281 actctctaca ggaaatcagc atctctagat agtcctgttc ttttggtcat caaagacatg
     5341 gataaccaga tatttggggc atatgcaact catcccttca agttcagtga ccactattat
     5401 ggcacaggcg aaacgtttct ctacaccttt agtccgaatt tcaaggtctt taagtggagt
     5461 ggagaaaact catattttat caatggagac ataagttctt tagaacttgg tggtggaggg
     5521 ggacgatttg gtctgtggct agatgctgat ttatatcatg gacgcagcaa ctcttgcagt
     5581 acttttaata atgatatcct ttcaaaaaaa gaagacttca tagttcagga cctagaggta
     5641 tggacatttg agggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     5701 gactacaagg atgacgatga caagctcgag ggcggtggag gcagtggtgg aggcggtagc
     5761 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     5821 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     5881 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     5941 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     6001 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     6061 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6121 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6181 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     6241 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     6301 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6361 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6421 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     6481 cgggatccgg actagttaac gcgtcgacca gtggtcgacc ctgtggaatg tgtgtcagtt
     6541 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6601 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6661 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     6721 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6781 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6841 aggcctaggc ttttgcaaaa agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     6901 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6961 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     7021 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     7081 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     7141 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     7201 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     7261 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     7321 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     7381 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     7441 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     7501 aaggagcgca cgaccccatg catcgccagt cgacaatcaa cctctggatt acaaaatttg
     7561 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7621 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7681 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7741 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7801 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7861 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7921 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     7981 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8041 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     8101 ctccctttgg gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact
     8161 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     8221 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     8281 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8341 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8401 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     8461 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     8521 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8581 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8641 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8701 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8761 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     8821 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     8881 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     8941 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     9001 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     9061 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     9121 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     9181 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     9241 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     9301 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     9361 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     9421 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     9481 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     9541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     9601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     9661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     9721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     9781 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     9841 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     9901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     9961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    10021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    10081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    10141 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    10201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    10261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    10321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    10381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    10441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    10501 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    10561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    10621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    10681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    10741 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    10801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    10861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    10921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    10981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    11041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    11101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    11161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    11221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    11281 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    11341 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaag
//
