LOCUS       V028074                10579 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028074
VERSION     V028074
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    113..134
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        149..179
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    187..203
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     211..227
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        248..266
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        294..520
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             521..701
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    748..873
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1366..1599
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1784..1828
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2095..2211
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     enhancer        2234..2537
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2538..2741
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2824..4905
                     /label="NFE2L3(NM_004289)"
                     /note="NFE2L3(NM_004289)"
                     /gene="NFE2L3"
     CDS             4942..5661
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        5711..6040
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6050..6649
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6716..7304
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7391..7624
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7696..7817
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7857..7992
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(8013..8031)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(8041..8057)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      8199..8654
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8680..8784
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8785..9645
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9816..10404
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
       61 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      241 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
      301 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
      361 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
      421 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
      481 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
      541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      721 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      781 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      901 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      961 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1021 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1081 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1141 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1201 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1261 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1321 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1381 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1441 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1501 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1561 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1621 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1681 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1741 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1801 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1861 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1921 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     1981 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2041 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     2101 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2161 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
     2221 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2401 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2461 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2521 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2641 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     2761 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagagcc
     2821 accatgaagc acctgaagcg gtggtggtcg gccggcggcg gcctcctgca cctcaccctc
     2881 ctgctgagct tggcggggct ccgcgtagac ctagatcttt acctgctgct gccgccgccc
     2941 accctgctgc aggacgagct gctgttcctg ggcggcccgg ccagctccgc ctacgcgctc
     3001 agccccttct cggcctcggg agggtggggg cgcgcgggcc acttgcaccc caagggccgg
     3061 gagctggacc ctgccgcgcc gcccgagggc cagctgctcc gggaggtgcg cgcgctcggg
     3121 gtccccttcg tccctcgcac cagcgtggat gcatggctgg tgcacagcgt ggctgccggg
     3181 agcgcggacg aggcccacgg gctgctcggc gccgccgccg cctcgtccac cggaggagcc
     3241 ggcgccagcg tggacggcgg cagccaggct gtgcaggggg gcggcgggga cccccgagcg
     3301 gctcggagtg gccccttgga cgccggggaa gaggagaagg cacccgcgga accgacggct
     3361 caggtgccgg acgctggcgg atgtgcgagc gaggagaatg gggtactaag agaaaagcac
     3421 gaagctgtgg atcatagttc ccagcatgag gaaaatgaag aaagggtgtc agcccagaag
     3481 gagaactcac ttcagcagaa tgatgatgat gaaaacaaaa tagcagagaa acctgactgg
     3541 gaggcagaaa agaccactga atctagaaat gagagacatc tgaatgggac agatacttct
     3601 ttctctctgg aagacttatt ccagttgctt tcatcacagc ctgaaaattc actggagggc
     3661 atctcattgg gagatattcc tcttccaggc agtatcagtg atggcatgaa ttcttcagca
     3721 cattatcatg taaacttcag ccaggctata agtcaggatg tgaatcttca tgaggccatc
     3781 ttgctttgtc ccaacaatac atttagaaga gatccaacag caaggacttc acagtcacaa
     3841 gaaccatttc tgcagttaaa ttctcatacc accaatcctg agcaaaccct tcctggaact
     3901 aatttgacag gatttctttc accggttgac aatcatatga ggaatctaac aagccaagac
     3961 ctactgtatg accttgacat aaatatattt gatgagataa acttaatgtc attggccaca
     4021 gaagacaact ttgatccaat cgatgtttct cagctttttg atgaaccaga ttctgattct
     4081 ggcctttctt tagattcaag tcacaataat acctctgtca tcaagtctaa ttcctctcac
     4141 tctgtgtgtg atgaaggtgc tataggttat tgcactgacc atgaatctag ttcccatcat
     4201 gacttagaag gtgctgtagg tggctactac ccagaaccca gtaagctttg tcacttggat
     4261 caaagtgatt ctgatttcca tggagatctt acatttcaac acgtatttca taaccacact
     4321 taccacttac agccaactgc accagaatct acttctgaac cttttccgtg gcctgggaag
     4381 tcacagaaga taaggagtag ataccttgaa gacacagata gaaacttgag ccgtgatgaa
     4441 cagcgtgcta aagctttgca tatccctttt tctgtagatg aaattgtcgg catgcctgtt
     4501 gattctttca atagcatgtt aagtagatat tatctgacag acctacaagt ctcacttatc
     4561 cgtgacatca gacgaagagg gaaaaataaa gttgctgcgc agaactgtcg taaacgcaaa
     4621 ttggacataa ttttgaattt agaagatgat gtatgtaact tgcaagcaaa gaaggaaact
     4681 cttaagagag agcaagcaca atgtaacaaa gctattaaca taatgaaaca gaaactgcat
     4741 gacctttatc atgatatttt tagtagatta agagatgacc aaggtaggcc agtcaatccc
     4801 aaccactatg ctctccagtg tacccatgat ggaagtatct tgatagtacc caaagaactg
     4861 gtggcctcag gccacaaaaa ggaaacccaa aagggaaaga gaaagctcga gggcggtgga
     4921 ggcagtggtg gaggcggtag catggtgagc aagggcgagg agctgttcac cggggtggtg
     4981 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     5041 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     5101 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     5161 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     5221 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     5281 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5341 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     5401 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     5461 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5521 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     5581 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     5641 atggacgagc tgtacaagta acgggatccg gactagttaa cgcgtcgacc agtggtcgac
     5701 cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     5761 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     5821 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5881 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5941 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6001 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttacca tgaccgagta
     6061 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     6121 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     6181 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6241 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6301 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6361 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6421 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6481 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6541 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6601 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
     6661 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgccag tcgacaatca
     6721 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6781 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6841 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6901 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6961 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7021 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7081 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     7141 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7201 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7261 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg
     7321 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7381 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     7441 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7501 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7561 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7621 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7681 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7741 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7801 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     7861 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7921 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7981 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     8041 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     8101 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     8161 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     8221 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8281 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8341 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8401 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8461 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8521 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8581 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     8641 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8701 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8761 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8821 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8881 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8941 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     9001 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     9061 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     9121 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     9181 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     9241 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     9301 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     9361 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     9421 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9481 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9541 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9601 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9661 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9721 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9781 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9841 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9901 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9961 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    10021 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    10081 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    10141 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    10201 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    10261 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    10321 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    10381 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    10441 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10501 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    10561 gagtcagtga gcgaggaag
//
