LOCUS       V036714                10597 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036714
VERSION     V036714
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    113..134
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        149..179
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    187..203
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     211..227
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        248..266
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        294..520
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             521..701
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    748..873
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1366..1599
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1784..1828
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2095..2211
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     enhancer        2234..2537
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2538..2741
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2818..2827
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2827..2892
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             2893..4923
                     /label="FOXP1(NM_032682)"
                     /note="FOXP1(NM_032682)"
                     /gene="FOXP1"
     CDS             4960..5679
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        5729..6058
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6068..6667
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6734..7322
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7409..7642
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7714..7835
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7875..8010
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(8031..8049)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(8059..8075)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      8217..8672
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8698..8802
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8803..9663
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9834..10422
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
       61 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      241 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
      301 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
      361 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
      421 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
      481 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
      541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      721 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      781 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      901 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      961 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1021 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1081 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1141 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1201 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1261 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1321 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1381 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1441 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1501 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1561 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1621 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1681 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1741 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1801 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1861 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1921 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     1981 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2041 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     2101 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2161 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
     2221 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2401 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2461 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2521 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2641 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     2761 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagagcc
     2821 accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     2881 gacgatgaca agatgatgca agaatctggg actgagacaa aaagtaacgg ttcagccatc
     2941 cagaatgggt cgggcggcag caaccactta ctagagtgcg gcggtcttcg ggaggggcgg
     3001 tccaacggag agacgccggc cgtggacatc ggggcagctg acctcgccca cgcccagcag
     3061 cagcagcaac aggcacttca ggtggcaaga cagctccttc ttcagcagca acagcagcag
     3121 caagttagtg gattaaaatc tcccaagagg aatgacaaac aaccagctct tcaggttccc
     3181 gtgtcagtgg ctatgatgac acctcaagtt atcactcccc agcaaatgca gcagatcctc
     3241 cagcaacaag tgctgagccc tcagcagctc caggttctcc tccagcagca gcaggccctc
     3301 atgcttcaac agcagcagct tcaagagttt tataaaaaac aacaggaaca gttgcagctt
     3361 caacttttac aacaacaaca tgctggaaaa cagcctaaag agcaacagca ggtggctacc
     3421 cagcagttgg cttttcagca gcagctttta cagatgcagc agttacagca gcagcacctc
     3481 ctgtctttgc agcgccaagg ccttctgaca attcagcccg ggcagcctgc ccttcccctt
     3541 caacctcttg ctcaaggcat gattccaaca gaactgcagc agctctggaa agaagtgaca
     3601 agtgctcata ctgcagaaga aaccacaggc aacaatcaca gcagtttgga tctgaccacg
     3661 acatgtgtct cctcctctgc accttccaag acctccttaa taatgaaccc acatgcctct
     3721 accaatggac agctctcagt ccacactccc aaaagggaaa gtttgtccca tgaggagcac
     3781 ccccatagcc atcctctcta tggacatggt gtatgcaagt ggccaggctg tgaagcagtg
     3841 tgcgaagatt tccaatcatt tctaaaacat ctcaacagtg agcatgcgct ggacgataga
     3901 agtacagccc aatgtagagt acaaatgcag gttgtacagc agttagagct acagcttgca
     3961 aaagacaaag aacgcctgca agccatgatg acccacctgc atgtgaagtc tacagaaccc
     4021 aaagccgccc ctcagccctt gaatctggta tcaagtgtca ctctctccaa gtccgcatcg
     4081 gaggcttctc cacagagctt acctcatact ccaacgaccc caaccgcccc cctgactccc
     4141 gtcacccaag gcccctctgt catcacaacc accagcatgc acacggtggg acccatccgc
     4201 aggcggtact cagacaaata caacgtgccc atttcgtcag cagatattgc gcagaaccaa
     4261 gaattttata agaacgcaga agttagacca ccatttacat atgcatcttt aattaggcag
     4321 gccattctcg aatctccaga aaagcagcta acactaaatg agatctataa ctggttcaca
     4381 cgaatgtttg cttacttccg acgcaacgcg gccacgtgga agaatgcagt gcgtcataat
     4441 cttagtcttc acaagtgttt tgtgcgagta gaaaacgtta aaggggcagt atggacagtg
     4501 gatgaagtag aattccaaaa acgaaggcca caaaagatca gtggtaaccc ttcccttatt
     4561 aaaaacatgc agagcagcca cgcctactgc acacctctca atgcagcttt acaggcttca
     4621 atggctgaga atagtatacc tctatacact accgcttcca tgggaaatcc cactctgggc
     4681 aacttagcca gcgcaatacg ggaagagctg aacggggcaa tggagcatac caacagcaac
     4741 gagagtgaca gcagtccagg cagatctcct atgcaagccg tgcatcctgt acacgtcaaa
     4801 gaagagcccc tcgatccaga ggaagctgaa gggcccctgt ccttagtgac aacagccaac
     4861 cacagtccag attttgacca tgacagagat tacgaagatg aaccagtaaa cgaggacatg
     4921 gagctcgagg gcggtggagg cagtggtgga ggcggtagca tggtgagcaa gggcgaggag
     4981 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     5041 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     5101 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     5161 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     5221 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     5281 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     5341 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     5401 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     5461 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     5521 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     5581 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     5641 gccgggatca ctctcggcat ggacgagctg tacaagtaac gggatccgga ctagttaacg
     5701 cgtcgaccag tggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     5761 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     5821 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5881 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5941 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6001 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6061 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     6121 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6181 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6241 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6301 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6361 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6421 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6481 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6541 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6601 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6661 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
     6721 atcgccagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6781 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6841 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6901 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6961 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7021 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7081 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     7141 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7201 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7261 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7321 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7381 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7441 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7501 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7561 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7621 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     7681 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7741 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7801 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     7861 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7921 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7981 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     8041 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     8101 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8161 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     8221 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8281 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8341 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8401 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8461 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8521 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8581 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8641 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     8701 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8761 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8821 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8881 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8941 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9001 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     9061 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9961 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    10381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    10441 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    10501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    10561 cgaacgaccg agcgcagcga gtcagtgagc gaggaag
//
