LOCUS       V038327                11326 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038327
VERSION     V038327
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6180
                     /label="Ptprc(NM_001111316)"
                     /note="Ptprc(NM_001111316)"
                     /gene="Ptprc"
     CDS             6187..6252
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6296..6507
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6520..6788
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6815..7414
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7421..8009
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8083..8316
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8388..8509
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8528..8663
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8701..8717)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8725..8741
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8749..8779)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8794..8815
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9103..9691)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9862..10722)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10723..10827)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11301..11317
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgaccatg ggtttgtggc tcaaacttct ggcctttgga
     2341 tttgcccttc tggacacaga agtctttgtc acagggcaaa cacctacacc cagtgatgaa
     2401 ctgagcacaa cagagaatgc ccttcttctg cctcaaagtg accccttacc tgctcgcacc
     2461 actgaatcca cacccccaag catctctgaa agaggaaatg gctcttcaga gaccacatat
     2521 catccaggtg tgttatccac gctgctgcct cacctgtccc cacagcctga ctcgcagacg
     2581 ccctctgccg gaggagctga cactcagaca ttcagcagcc aagctgacaa tcccacactc
     2641 acgcctgctc ccggcggcgg gactgaccca ccaggtgtgc caggggagag gactgtaccg
     2701 gggaccattc ctgcagacac agcctttcct gttgataccc ccagccttgc acgcaacagc
     2761 tctgctgcct cacctacaca cacctccaat gtcagcacca cagatatctc ttcaggtgcc
     2821 agcctcacaa ctcttacacc atccactctg ggccttgcaa gcactgaccc tccaagcaca
     2881 accatagcta ccacaacgaa gcaaacatgt gctgccatgt ttgggaacat tactgtgaat
     2941 tacacctatg aatctagtaa tcagactttt aaggcagacc tcaaagatgt ccaaaatgct
     3001 aagtgtggaa atgaggattg tgaaaacgtg ttaaataatc tagaagaatg ctcacagata
     3061 aaaaacatca gtgtgtctaa tgactcatgt gctccagcta caactataga tttatatgta
     3121 ccaccaggga ctgacaagtt ttcgctacat gactgcacac caaaagaaaa ggctaatact
     3181 tcaatttgtt tggagtggaa aacaaaaaac cttgatttca gaaaatgcaa cagtgacaat
     3241 atttcatatg tactccactg tgagccagaa aataatacaa aatgcattag aagaaataca
     3301 ttcatacctg aaagatgtca gttggacaac cttcgtgccc aaacaaatta cacatgtgta
     3361 gcagaaatct tatatcgcgg tgtaaaactc gtcaaaaatg ttataaatgt gcagacagat
     3421 ttggggattc cagaaacgcc taagcctagt tgtggggatc cagctgcaag aaaaacgtta
     3481 gtctcttggc ctgagcctgt atctaaacct gagtctgcat ctaaacccca tggatatgtt
     3541 ttatgctata agaacaattc agaaaaatgt aaaagtttgc ctaataatgt gaccagtttt
     3601 gaggtggaaa gcttgaaacc ttataaatac tatgaagtgt ccctacttgc ctatgtcaat
     3661 gggaagattc aaagaaatgg gactgctgag aagtgcaatt ttcacacaaa agcagatcgt
     3721 ccggacaagg tcaatggaat gaaaacctcc cggccgacag acaatagtat aaatgttaca
     3781 tgtggtcctc cttatgaaac taatggccct aaaacctttt acattttggt agtcagaagt
     3841 ggaggttctt ttgttacaaa atacaacaag acaaactgtc agttttatgt agataatctc
     3901 tactattcaa ctgactatga gtttctggtc tcttttcaca atggagtgta cgagggagat
     3961 tcagttataa gaaatgagtc aacaaatttt aatgctaaag cactgattat attcctggtg
     4021 tttctgatta ttgtgacatc aatagccttg cttgttgttt tgtataaaat ctatgatctg
     4081 cgcaagaaaa gatccagcaa tttagatgaa caacaggaac tcgttgaaag ggatgatgaa
     4141 aagcagctga tggatgtgga gccaatccat tctgacattt tgttggaaac atacaaaagg
     4201 aagattgctg atgagggcag actgttcctg gctgaatttc agagcattcc acgggtattc
     4261 agcaagtttc ccatcaaaga tgcccgaaag ccccacaatc agaataaaaa ccgttatgtt
     4321 gacattcttc cctatgatta taaccgtgtg gaactctctg aaataaatgg agatgcaggg
     4381 tccacctaca taaatgccag ctacattgat ggcttcaagg aacccaggaa atacattgct
     4441 gcacaagggc cccgggatga gacagttgat gacttctgga ggatgatctg ggagcaaaag
     4501 gccacagtta ttgtcatggt cacacgatgt gaagaaggaa acaggaacaa gtgcgcagaa
     4561 tactggccaa gcatggagga aggcactcgg gctttcaaag atattgttgt gacaatcaat
     4621 gaccacaaac gatgtcctga ttacatcatt cagaagctga acgttgcaca taaaaaagaa
     4681 aaagcaactg gaagagaagt gactcatatc caattcacca gctggccaga ccatggggtt
     4741 cctgaagacc ctcacctgct cctcaaactt cgacggagag ttaatgcttt tagcaacttc
     4801 ttcagtggtc ccattgtggt gcactgcagt gctggtgttg ggcgtacagg tacctacatt
     4861 ggaattgatg ccatgctgga aggcctggaa gcagagggca aagtggatgt ctatggttat
     4921 gttgtcaagc taaggcgaca gaggtgtctg atggtgcaag tggaggcaca gtatatcctg
     4981 attcatcagg ctttagtgga atacaatcag tttggagaaa cagaagtgaa cttgtctgag
     5041 ttacattcat gcctacacaa catgaagaag agagatccac ccagtgaccc ctcccctctg
     5101 gaggctgaat accagagact tccttcatac aggagttgga ggacacagca cattggaaat
     5161 caagaagaaa ataagaagaa gaacaggaat tctaatgttg ttccatatga ctttaacaga
     5221 gtgccactta agcatgaact ggagatgagc aaagagagtg agcctgaatc agatgagtct
     5281 tcagatgatg acagtgactc agaagaaacc agcaaataca ttaatgcatc ctttgtgatg
     5341 agttactgga aaccagaaat gatgattgct gctcaggggc cactaaaaga aacgatcggt
     5401 gacttttggc agatgatatt ccaaagaaaa gtcaaagtta ttgtgatgtt gacagagtta
     5461 gtgaatggag accaggaagt ctgtgctcag tactggggcg aaggaaagca gacttatgga
     5521 gacatggaag tggagatgaa agacacaaac agagcctcag cctacactct ccgaactttt
     5581 gagctgagac attccaagag gaaggagccc agaactgtgt accagtacca gtgtaccaca
     5641 tggaaagggg aagagctgcc tgcagaaccc aaagacctgg tgtctatgat tcaggacctc
     5701 aaacagaagc ttcccaaggc ttccccagaa gggatgaagt atcacaagca tgcatccatc
     5761 ctcgtccact gcagagatgg atcccagcag acagggttgt tctgtgcctt gttcaatctc
     5821 ttggaaagtg cagaaacaga agatgtggtt gatgttttcc aagtggtaaa gtctctacgc
     5881 aaagcacggc ctggggtggt gtgcagctat gagcaatacc agttcctcta tgacatcatc
     5941 gccagcatct atcccgccca gaatggacaa gtcaagaaaa caaacagcca agacaaaatt
     6001 gaatttcata atgaagtgga tggaggcaag caggatgcta actgtgtccg tccagatggt
     6061 cctctgaata aagcccagga agacagcaga ggggtgggaa ccccggagcc taccaatagt
     6121 gctgaggaac cagaacatgc tgccaatggt tctgcgagcc cagctccaac ccagagttca
     6181 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     6241 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     6301 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     6361 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     6421 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     6481 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     6541 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     6601 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     6661 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     6721 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     6781 tacagatcca agctgtgacc ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg
     6841 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga
     6901 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct
     6961 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     7021 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     7081 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7141 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     7201 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     7261 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     7321 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     7381 cacctggtgc atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa
     7441 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7501 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7561 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7621 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7681 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7741 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7801 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7861 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7921 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7981 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg
     8041 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     8101 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     8161 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8221 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8281 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     8341 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     8401 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8461 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     8521 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8581 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8641 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg
     8701 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8761 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8821 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8941 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9001 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9541 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9721 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9781 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9841 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9901 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9961 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10021 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10081 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10141 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10201 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10261 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10321 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10381 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10441 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10501 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10561 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10621 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10681 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10741 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10801 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    10861 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10921 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    10981 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11041 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11101 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11161 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11221 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11281 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
