LOCUS       V031698                10660 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031698
VERSION     V031698
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4702
                     /label="Ptpn22(NM_008979)"
                     /note="Ptpn22(NM_008979)"
                     /gene="Ptpn22"
     CDS             4709..4774
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4819..5030
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5043..5311
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5340..5349
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5370..6032
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6102..6155
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6156..6755
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6756..7343
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7417..7650
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7722..7843
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7862..7997
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8035..8051)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8059..8075
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8083..8113)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8128..8149
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8437..9025)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9196..10056)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10057..10161)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10635..10651
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg accaaagaga aattctgcag caactactga aagaagccca
     2341 gaaaaagaaa ctcaacagtg aggagtttgc cagtgaattt ctgaagctga aaaggcaatc
     2401 caccaagtac aaggccgaca aaatctatcc tacaactgtg gctcagaggc ccaagaatat
     2461 caagaaaaac agatacaagg atattttgcc ctatgatcac agcctggtag agctgtctct
     2521 gttaacttcc gatgaggatt ccagttatat caatgccagc tttattaagg gtgtctatgg
     2581 acccaaggct tatattgcta cccagggtcc tttatctaca actctcctgg acttctggag
     2641 gatgatttgg gagtaccgca tcttggtcat tgtcatggca tgtatggagt ttgaaatggg
     2701 aaagaaaaaa tgtgagcgtt attgggccga accaggagaa acgcagctgc aatttggccc
     2761 cttttctata tcctgtgaag ctgagaaaaa gaaatctgat tataaaatca ggactctgaa
     2821 ggccaagttc aataatgaaa ctcgaattat ttaccagttt cattataaga attggccaga
     2881 ccatgatgtg ccttcatcta tagaccctat tcttcagctc atctgggata tgcgttgtta
     2941 ccaagaagat gactgtgttc ctatatgcat tcactgcagt gccggctgcg gaaggacagg
     3001 tgtcatttgt gctgttgatt atacatggat gctgctgaaa gatgggataa ttcctaagaa
     3061 cttcagtgtt tttaatttga ttcaggagat gcgaacacag aggccttcgc tagttcaaac
     3121 tcaggaacag tacgaactgg tctacagtgc tgtgttagag ctgtttaaga ggcacatgga
     3181 tgttatctct gataatcacc ttggaagaga gattcaagca caatgctcaa ttcctgaaca
     3241 aagcctcacg gtagaagctg actcttgtcc tctggattta ccaaaaaacg ccatgaggga
     3301 tgtgaaaacg acaaaccagc atagcaaaca aggggctgaa gcggagagca ctggagggtc
     3361 ttcccttggc cttaggactt ctacgatgaa tgccgaggaa gagttggttt tgcactcggc
     3421 taaatcaagc ccttctttta actgtttaga gctaaactgc gggtgtaaca acaaggctgt
     3481 cataaccagg aacgggcagg caagggcttc tccagtcgtg ggagagcccc ttcagaagta
     3541 tcaaagtctg gattttggtt ccatgttgtt tgggtcgtgt cctagtgctc tgcccataaa
     3601 cacagcggac aggtatcaca attcaaaggg gccggtaaaa cggaccaaat caactccctt
     3661 tgaactgatt cagcagagaa aaacaaatga cttggccgtg ggagacggtt tttcatgcct
     3721 ggaatctcag ctgcatgagc attacagtct cagggagctg caggtgcaaa gagtggccca
     3781 tgtttcttca gaagagctga attattcact gcctggtgcc tgtgatgcgt cgtgtgtgcc
     3841 ccggcacagc cccggcgctt tgagagtgca tctgtacaca tctttagcgg aagatcctta
     3901 tttttcatca tcccctccga atagtgctga ttcgaagatg tcttttgatc tgcctgagaa
     3961 acaggatgga gccacttccc ctggcgctct attgccagcc tcttctacaa cctccttctt
     4021 ttatagcaac ccacacgact ccctagtgat gaacactctg accagctttt ccccaccgtt
     4081 aaaccaagag acagctgtag aagctccttc tcggaggaca gatgatgaaa tccccccgcc
     4141 actccctgaa cggacacccg agtcttttat tgtggttgag gaagccggag agccctcacc
     4201 acgtgttacc gaatccttac ctctggtggt aacatttgga gcatcaccag aatgcagtgg
     4261 gacatctgaa atgaagagcc atgactctgt agggtttaca ccaagcaaga atgtgaaact
     4321 ccgaagtccc aaatcagatc gacatcagga tggttctcct ccacctcctc tcccagaaag
     4381 aactctagag tccttctttc ttgctgatga ggactgtata caggcccaag ccgtgcaaac
     4441 ttcttctact agctatcctg aaaccacaga gaactccaca tcttctaaac aaacattgag
     4501 gacccctgga aaaagtttca caaggagtaa gagtttgaag atttttcgaa atatgaaaaa
     4561 aagtgtttgt aattcttcct caccaagcaa gcctacagaa cgtgttcagc caaaaaattc
     4621 cagctccttt ctgaattttg gtttcggaaa tcgtttttca aaacccaaag gaccaaggaa
     4681 cccgccatca gcttggaata tgggatccga ctacaaagac catgacggtg attataaaga
     4741 tcatgacatc gactacaagg atgacgatga caagtgagcg gccgcgaagg atctgcgatc
     4801 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     4861 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     4921 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     4981 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc
     5041 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
     5101 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
     5161 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
     5221 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
     5281 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg
     5341 ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg
     5401 gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccaagcagg
     5461 gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc
     5521 tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga
     5581 accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg
     5641 aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg
     5701 gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga
     5761 tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac gtgctggtgg
     5821 gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc gtggtggaca
     5881 gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt
     5941 tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc gtggagtacc
     6001 agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg tccaattctg
     6061 ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga ggaagtcttc
     6121 taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc
     6181 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     6241 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     6301 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     6361 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     6421 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     6481 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     6541 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6601 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6661 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6721 gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt
     6781 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6841 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6901 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6961 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7021 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7081 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7141 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     7201 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7261 gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga
     7321 tctccctttg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     7381 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7441 gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7501 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7561 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7621 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7681 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7741 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7801 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7861 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7921 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7981 aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata
     8041 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8101 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8161 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8221 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8281 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8341 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8401 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8461 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8521 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8581 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8641 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8701 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8761 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8821 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8881 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8941 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9001 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9061 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9121 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9181 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9241 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9301 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9361 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9421 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9481 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9541 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9601 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9661 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9721 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9781 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9841 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9901 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9961 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10021 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10081 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10141 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10201 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10261 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10321 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10381 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    10441 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    10501 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10561 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10621 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
