LOCUS       V023694                10106 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023694
VERSION     V023694
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4107
                     /label="Ptpn11(NM_011202)"
                     /note="Ptpn11(NM_011202)"
                     /gene="Ptpn11"
     CDS             4114..4140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4144..4170
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4174..4200
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4265..4476
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4489..4757
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4786..4795
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4816..5478
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5548..5601
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5602..6201
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6202..6789
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6863..7096
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7168..7289
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7308..7443
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7481..7497)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7505..7521
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7529..7559)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7574..7595
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7883..8471)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8642..9502)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9503..9607)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10081..10097
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga catcgcggag atggtttcac
     2341 cccaacatca ctggtgtgga ggcagagaat ctcctgctga ccagaggagt cgatggcagt
     2401 tttttagcaa ggcccagtaa gagtaaccct ggagacttca ctctgtctgt tagaagaaat
     2461 ggagctgtta cccacatcaa gattcagaac actggggact actatgacct ctatggtggg
     2521 gagaagtttg ccactttggc tgaactggtt cagtattaca tggaacacca tgggcagctg
     2581 aaagagaaga atggagatgt tatcgagctc aagtacccgc tgaactgtgc agaccctacc
     2641 tctgaaaggt ggttccatgg tcacttgtct ggaaaagaag cagagaagct gctgacggag
     2701 aagggcaagc atggcagctt cctcgttcga gagagccaga gccaccccgg agacttcgtt
     2761 ctctccgtgc gcactggtga cgacaaaggg gagagcaacg acggcaagtc caaagtgacc
     2821 cacgtcatga tccgctgtca ggagctgaaa tacgacgttg gtgggggaga gcgctttgac
     2881 tctctgacag acctggtgga gcattacaag aagaacccca tggtggagac gctgggcaca
     2941 gtcctgcagc tcaagcagcc cctcaacaca actcgtatca atgctgctga aattgaaagc
     3001 cgggttcgag agttaagcaa gctggctgag accacagata aagtcaagca gggcttttgg
     3061 gaagagtttg agacgctcca gcaacaggaa tgcaaacttc tctatagccg aaaagaagga
     3121 cagagacaag aaaataaaaa caaaaacaga tacaaaaaca tcctgccctt tgatcatacc
     3181 agggtcgttc tgcatgatgg ggatcccaat gagcctgttt ctgattacat taatgcaaac
     3241 atcatcatgc ctgagtttga gaccaagtgc aacaattcca aacccaaaaa gagttacatt
     3301 gccactcaag gctgcctgca gaacacggtg aatgacttct ggcggatggt gttccaggag
     3361 aactctcgag tcattgtcat gaccacaaag gaagtggaga gagggaagag caaatgtgtc
     3421 aagtactggc ctgatgagta tgcgctcaaa gaatacgggg tcatgcgtgt taggaacgtc
     3481 aaagaaagtg ccgcccatga ctacacttta cgagagctca aactctctaa ggtcggacaa
     3541 gctctactcc agggaaacac agagagaacc gtctggcagt accactttcg gacctggcca
     3601 gaccatggcg tgcctagtga ccctggaggt gtgctggact tcctggagga ggtccaccac
     3661 aagcaggaga gcatcgtgga tgcaggccct gtcgtggttc actgcagcgc tgggattggc
     3721 cggacaggaa ccttcattgt gattgacatc cttattgaca tcattcgaga gaaaggtgtg
     3781 gactgtgaca tcgacgttcc taaaaccatt cagatggtgc ggtcccagag gtcggggatg
     3841 gtccagacag aagcacagta ccggtttatc tacatggctg tccagcacta catagagacg
     3901 ctgcagcgcc ggatcgagga ggagcagaaa agcaaaagaa aaggacatga atataccaat
     3961 attaagtatt ccttggtgga ccagacaagt ggtgatcaga gtcccctgcc accctgcacc
     4021 ccaacgccac cctgtgcaga aatgagggag gacagcgccc gagtctatga gaacgtgggc
     4081 ctcatgcagc agcagaggag tttcagactc gagtacccat acgacgtacc tgattatgca
     4141 acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca
     4201 tagggatccc tgcgatatcc agcgcggccg cgaaggatct gcgatcgctc cggtgcccgt
     4261 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     4321 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     4381 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     4441 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     4501 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     4561 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     4621 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     4681 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     4741 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     4801 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     4861 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     4921 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     4981 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     5041 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     5101 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     5161 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     5221 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     5281 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     5341 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     5401 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     5461 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     5521 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     5581 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     5641 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5701 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5761 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5821 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5881 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5941 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6001 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6061 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6121 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6181 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     6241 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6301 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6361 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6421 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6481 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6541 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     6601 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6661 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6721 ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttggccg
     6781 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6841 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg
     6901 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6961 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7021 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7081 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     7141 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7201 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7261 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     7321 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7381 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7441 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt
     7501 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7561 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7621 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7681 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7741 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7801 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7861 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7921 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7981 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8041 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8101 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8161 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8221 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8281 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     8341 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8401 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8461 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8521 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8581 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8641 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8701 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8761 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8821 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8881 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8941 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9001 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9061 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9121 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9181 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9241 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9301 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9361 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9421 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9481 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9541 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9601 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     9661 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     9721 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     9781 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     9841 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     9901 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     9961 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
    10021 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    10081 gtaaaacgac ggccagtgcc aagctg
//
