LOCUS       V020811                10580 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020811
VERSION     V020811
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2293..5478
                     /label="Ptk7(NM_175168)"
                     /note="Ptk7(NM_175168)"
                     /gene="Ptk7"
     CDS             5479..5502
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     promoter        5550..5761
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5774..6042
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6069..6668
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6675..7263
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7337..7570
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7642..7763
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7782..7917
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7955..7971)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7979..7995
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8003..8033)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8048..8069
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8357..8945)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9116..9976)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9977..10081)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10555..10571
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcca ccatgggagc ccgcccgctg actctgctca gagccctgct tctgccgctg
     2341 ctggccggtg cccaagccgc tatcgtcttt atcaaggagc cgtcctccca ggatgcactt
     2401 caagggcgcc gggcgctgct ccgttgtgaa gtggaggctc cggacccggt gcacgtgtac
     2461 tggctgctca atggggtgcc tgtccaggac acggagcgac ggtttgccca gggcagcagc
     2521 ctgagtttcg cagctgtgga ccggctccag gattctggtg ctttccagtg tgtggctcgg
     2581 gataacgtca ctggagagga agtccgcagt actaatgcct ccttcaacat caaatggatc
     2641 gaagcaggtc ctgtggtcct gaagcaccca gcctcagaag ctgagatcca gccccagacc
     2701 caggtcacac tgcgatgtca cattgacggg caccctcggc ctacctacca gtggttccga
     2761 gatgggaccc ccctctctga tgatcagagc acccacaccg tcagcagcag ggagaggaac
     2821 ctgaccctac ggccagccag tcccgaacat agtggcctct actcctgctg tgcccacaat
     2881 gcctttggcc aggcctgcag cagccagaac ttcaccttga gtgttgcaga tgaaagcttt
     2941 gccagggtgg tgctggcacc tcaggatgtt gttgtagcaa ggaacgagga ggccatgttc
     3001 cattgccagt tttcagccca gccacccccg agcctgcagt gggtctttga ggacgagact
     3061 cccatcacta accgaagccg tccccctcac ctccgcagag ctgtggtgtt tgccaatggc
     3121 tctctgctgc tgacccaagt ccggccacgg aatgcaggcg tctaccgttg catcggtcag
     3181 ggacagaggg gcccacccat tgtcctggaa gccactctcc acctggcaga gattgaagac
     3241 atgctgccgt ttgaaccacg ggtgttcata gctggtgatg aggaacgagt cacctgccca
     3301 gccccccagg gcctgcctac acccagtgtc tggtgggagc atgctggagt ccctcttcct
     3361 gcccatggca gggtccacca gaagggcctt gagcttgtgt ttgtcaccat cgctgagagt
     3421 gatactggtg tctacacctg ccatgcaagc aacctggctg gtcagcggcg acaggatgtc
     3481 aatatcacag tggccactgt acccacatgg cttaggaagc cccaagacag ccagctggaa
     3541 gagggcaagc ctggctactt gcactgtctg acacaggcca cacccaagcc cactgtgatc
     3601 tggtacagga accagatgct gatctcagag gactcgaggt tcgaagtttc gaagaatggg
     3661 accttgcgca tcaatagtgt ggaggtgtat gacgggacat tgtaccgttg cgtgagcagt
     3721 accccagccg gcagcatcga ggcccaggcc cgagtccagg tgctggaaaa actcaagttc
     3781 acgccaccac cgcagccaca gcagtgcatg gagtttgata aggaggctac ggtgccctgc
     3841 tcagccactg gccgggagaa gcccacggtt aagtgggtac gggcagatgg aagtagcctc
     3901 cctgaatggg tgacagacaa tgctggaacc ctacactttg cccgagtgac ccgagatgat
     3961 gctggcaact acacttgcat tgcatccaac gagccacagg gccagatccg cgctcatgtc
     4021 cagctgacgg tggcagtttt catcaccttc aaagtggaac ctgagcgtac aactgtgtac
     4081 cagggccaca cagcgctgct gcggtgtgag gctcagggcg accccaagcc tctcattcag
     4141 tggaaaggca aagaccggat cctggaccct accaagctgg gacccaggat gcacatcttc
     4201 cagaacggtt ccctggtgat ccatgacgtg gcccctgagg actcaggctc ctacacctgc
     4261 atcgctggca acagctgtaa catcaggcac acagaggccc cgctcttagt cgtggacaag
     4321 ccagtgatgg aagactcgga gggcccaggc agccctcccc catacaagat gatccagacc
     4381 atcgggctgt cggtgggcgc agcggtagcc tacatcatcg cggtcctggg cctcatgttc
     4441 tactgcaaga agcggtgtaa agccaagcgg ctgcagaaac agcctgaggg ggaggagcca
     4501 gagatggagt gcctcaatgg tgggcctttg cagaatggtc agccatcagc cgagatccag
     4561 gaagaagtgg cgttgaccag cttgggctct ggccccccag ccaccaacaa gcgccacagc
     4621 gccggtgata ggatgcattt cccgagagcc agcctgcagc ctatcaccac tctggggaaa
     4681 agcgagttcg gggaggtgtt cctggcaaag gcgcagggcg tagaggaagg cgcgacggag
     4741 accctggtcc tcgtgaagag cctgcagagc agggacgagc aacaacagct ggacttccgg
     4801 agagaggtgg agatgttcgg gaagttgaac catgccaacg tggtgcggct tctggggctg
     4861 tgccgggagg ctgagcccca ctacatggtg ctggagtatg tggacctggg agatctcaaa
     4921 cagttcctga ggatttccaa gaacaaggat gaaaagttga agtcacagcc cctcagcacc
     4981 aaacagaagg tggccctgtg ttcccaggtg gccctgggca tggagcacct gtccaacaac
     5041 cgctttgtgc acaaggactt agcggctcgc aactgcctga tcagtgccca gaggcaggtg
     5101 aaggtgtccg cactgggcct gagcaaggat gtatacaaca gtgagtacta ccacttccgc
     5161 caggcctggg tacctctgcg ttggatgtct ccagaggctg tcctggaggg tgacttctcc
     5221 actaagtctg atgtctgggc ctttggtgtg ctcatgtggg aagtgttcac tcatggagag
     5281 atgccccatg gtggacaggc agatgatgag gtgctggcag acttgcaggc tggcaaggct
     5341 cgactcccgc agcccgaagg ctgcccgtct aagctctacc ggctgatgca gcgctgctgg
     5401 gccccgaacc ccaaggaccg gccctccttc agtgagatcg ccagcaccct gggagacagc
     5461 cctgcagaca gcaagcagga ttacaaggac gacgatgaca agtgatccgc ggccgcgaag
     5521 gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     5581 gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa
     5641 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     5701 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     5761 gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg
     5821 ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc
     5881 gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc
     5941 ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact
     6001 ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc
     6061 tacgctagat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     6121 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     6181 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6241 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6301 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     6361 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     6421 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     6481 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6541 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6601 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6661 gtgcctgagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6721 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6781 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6841 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6901 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6961 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7021 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     7081 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7141 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7201 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7261 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7321 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt
     7381 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7441 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7501 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7561 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7621 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     7681 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7741 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     7801 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7861 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7921 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     7981 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8041 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8101 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8161 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8221 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8281 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8341 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8401 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8461 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8521 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8581 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8641 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8701 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8761 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     8821 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8881 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8941 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9001 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9061 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9121 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9181 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9241 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9301 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9361 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9421 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9481 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9541 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9601 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9661 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9721 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9781 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9841 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9901 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9961 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10021 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10081 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    10141 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
    10201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    10261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
    10321 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    10381 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
    10441 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    10501 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    10561 cgacggccag tgccaagctg
//
