LOCUS       V045046                10032 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045046
VERSION     V045046
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4227
                     /label="Prmt5(NM_013768)"
                     /note="Prmt5(NM_013768)"
                     /gene="Prmt5"
     CDS             4234..4260
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4264..4290
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4294..4320
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4385..4596
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4609..4877
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4906..4915
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4936..5598
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5668..5721
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5722..6120
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    6127..6715
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6789..7022
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7094..7215
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7234..7369
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7407..7423)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7431..7447
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7455..7485)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7500..7521
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7809..8397)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8568..9428)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9429..9533)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10007..10023
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggcgatggc agtcggaggt
     2341 gctggtggca gccgcgtgtc cagcgggagg gacctgaatt gcgtccccga aatagctgac
     2401 acactgggtg ctgtggccaa gcaggggttt gatttcctct gcatgcctgt cttccacccg
     2461 cgtttcaaga gggagttcat tcaggaacct gctaagaatc ggcctggccc ccagacacga
     2521 tcagacctac tgctgtcagg aagggactgg aatacgctaa ttgtgggaaa gctttctcca
     2581 tggattcatc cagactcaaa agtggagaag atccgaagga actctgaagc ggctatgtta
     2641 caggagttga attttggggc atatctgggt cttccagctt tcctattgcc ccttaatcag
     2701 gaagataaca cgaatctggc cagagttctg accaaccaca tccacactgg ccaccactct
     2761 tccatgttct ggatgagggt acccttggtg gccccagagg acctgagaga tgatgtaatt
     2821 gagaatgccc cgactacaca cacagaggag tacagtggag aagagaaaac atggatgtgg
     2881 tggcataact ttcggactct gtgtgactat agcaagagaa ttgcagtagc tcttgaaatt
     2941 ggagctgacc tcccgtctaa tcacgtcatt gaccgctggc ttggagagcc catcaaagca
     3001 gccattctcc ccaccagcat tttcctaacc aacaagaaag gatttcctgt tctttctaag
     3061 gtgcagcaga ggctgatctt ccggctcctc aagttggaag tgcagtttat catcacggga
     3121 accaaccacc actcagagaa ggagttctgt tcctacctcc agtacttgga atacttaagc
     3181 caaaatcgcc ctccacccaa tgcctatgag ctctttgcca aaggctatga agactatctg
     3241 cagtccccac tccagcctct gatggacaat ctggaatctc agacatatga agtgtttgaa
     3301 aaggacccca tcaaatactc tcaatatcag caggctattt ataaatgttt gctagaccga
     3361 gtaccagaag aagaaaagga gaccaatgtc caggtactta tggtgctggg tgcaggccgg
     3421 ggtcctcttg tgaatgcgtc tcttcgggca gccaaacagg ccgagcggcg gatcaggctg
     3481 tatgctgtgg agaagaaccc caatgctgtg gtgacgctag agaactggca gtttgaagaa
     3541 tgggggagcc aggtgacagt tgtctcatca gacatgcggg aatgggtggc tccggagaaa
     3601 gctgacatca ttgtcagtga gcttctgggt tcctttgccg acaacgagct gtcacctgag
     3661 tgtctggatg gagcacagca cttcctgaaa gatgatggcg tgagcatccc tggagaatac
     3721 acctccttcc tggctcccat ttcttcctct aagctgtaca atgaggtccg tgcctgtcgg
     3781 gaaaaggacc gcgatcctga ggcacagttt gagatgcctt atgtggttcg gttgcacaac
     3841 ttccaccagc tctctgctcc taagccctgc tttaccttca gccatcccaa ccgagatcct
     3901 atgattgaca acaaccgcta ctgtaccttg gagtttcctg tggaggtgaa cacagtgctt
     3961 catggcttcg caggctactt tgagactgtg ctttaccggg acatcactct gagtatccgc
     4021 ccagagactc actctcctgg gatgttctca tggttcccca tcttcttccc cattaagcag
     4081 cccatcacgg tgcacgaagg ccagaacatc tgtgtgcgtt tctggcgatg cagcaattcc
     4141 aagaaagtgt ggtacgagtg ggcggtgacg gcccccgtct gttcttctat tcacaaccct
     4201 accggccgct cctataccat tggcctcctc gagtacccat acgacgtacc tgattatgca
     4261 acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca
     4321 tagggatccc tgcgatatcc agcgcggccg cgaaggatct gcgatcgctc cggtgcccgt
     4381 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     4441 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     4501 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     4561 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     4621 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     4681 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     4741 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     4801 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     4861 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     4921 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     4981 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     5041 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     5101 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     5161 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     5221 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     5281 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     5341 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     5401 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     5461 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     5521 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     5581 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     5641 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     5701 gtggaggaga atcccggccc tatggccaag cctttgtctc aagaagaatc caccctcatt
     5761 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     5821 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     5881 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     5941 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     6001 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     6061 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     6121 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6181 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6241 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6301 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6361 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6421 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6481 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6541 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6601 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6661 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     6721 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6781 ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac
     6841 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6901 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6961 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7021 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7081 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7141 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7201 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc
     7261 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7321 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     7381 agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7441 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7501 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7561 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7621 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7681 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7741 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7801 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7861 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7921 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7981 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8041 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8101 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8161 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8221 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8281 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8341 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8401 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8461 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8521 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8581 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8641 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8701 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8761 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8821 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8881 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8941 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9001 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9061 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9121 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9181 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9241 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9301 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9361 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9421 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9481 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9541 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     9601 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     9661 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     9721 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     9781 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     9841 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     9901 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     9961 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
    10021 agtgccaagc tg
//
