LOCUS       V025229                13319 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025229
VERSION     V025229
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7362
                     /label="Plch1(NM_183191)"
                     /note="Plch1(NM_183191)"
                     /gene="Plch1"
     CDS             7369..7434
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7478..7689
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7702..7970
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7999..8008
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8029..8691
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8761..8814
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8815..9414
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9415..10002
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10076..10309
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10381..10502
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10521..10656
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10694..10710)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10718..10734
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10742..10772)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10787..10808
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11096..11684)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11855..12715)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12716..12820)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13294..13310
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cagaccttga agtgtataaa
     2341 aacttaagtc cagaaaaagt tgaaagatgc atgagtgtaa tgcagtccgg gacacagatg
     2401 atcaaactga agcgtggcac caaagggctc gttcgcctct tttacctgga tgagcaccgg
     2461 acccgcctcc gatggcgacc ctctcggaag agtgagaagg caaaaatact tattgactcc
     2521 atctacaaag tcaccgaggg caggcagtct gaaatattcc acagacaggc tgaggggaac
     2581 tttgacccta gctgctgctt taccatctac catggcaacc acatggagtc tctggacctc
     2641 attacctcca acccagagga ggcacgcacc tggatcacgg gcctcaagta tctgatggct
     2701 ggcatcagtg atgaagactc ccttgccaag aggcagagga cccatgacca atgggtgaag
     2761 cagacctttg aggaagctga taagaacggt gatggcttat tgaatattga agagattcac
     2821 cagctgatgc ataaactaaa tgtcaatctg ccccgcagaa aagtcaggca aatgtttcag
     2881 gaagcagata cagatgagaa tcagggaacc ttgacatttg aagagttctg cgttttttac
     2941 aaaatgatgt cattgagacg agacctctat ttgctgctct tgagctacag tgacaagaaa
     3001 gaccacctga ctgtggagga gctggctcag tttttgaaag tggaacaaaa gatgagtaat
     3061 gtgacactgg actattgtct tgacatcata atgaagtttg aagtttctga agaaaacaaa
     3121 gtgaaaaacg tccttggtat agaaggcttc acgaacttca tgcgtagccc tgcctgtgac
     3181 gtatttaacc cgttgcacca tgaagtgtac caagacatgg atcagcccct gtgcaactac
     3241 tacattgctt catctcacaa cacatacctg actggggatc agctcctttc tcaatctaaa
     3301 gtggatatgt atgcacgggt gctacaagag ggttgtagat gcgtggaagt tgactgttgg
     3361 gatggcccag atggagagcc agtggtccac catggttata ctctcacttc aaaaattctc
     3421 ttcagagatg ttgtggagac catcaacaag catgcattcg tgaagaatga gttcccggtt
     3481 atcctgtcca ttgagaacca ctgcagcatt cagcagcaga ggaagattgc tcagtacctg
     3541 aaaggtatat tgcaggacaa actggacctg tcttctgtag acaccggaga gtgcaggcag
     3601 cttccaagcc ctcagagtct gaaaggcaaa atcctagtga agggcaagaa gttgccgtat
     3661 caccttgggg atgatgcaga ggaaggagaa gtgtccgatg aggacagtgc agatgagatt
     3721 gaagatgagt gcaagttcaa gctgcattac agtaacggga ccactgagca ccaggtagaa
     3781 tctttcatac ggaaaaagct ggagtcactg ttgaaggagt ctcagattcg agacaaagaa
     3841 gatccagaca gtttcacagt gagggcgtta ctcaaggcta cacatgaagg cttaaatgca
     3901 cacctgaagc agaacctgga tgtaaaggaa agtggaaaga agtcccatgg gcgatccctg
     3961 atggccaact ttgggaaaca caagcagaaa gctacgaaat cacgttctaa atcctatagt
     4021 actgatgatg aggatgatag ccttcagaat cctggcaagg agggaggcca gctgtacagg
     4081 ctgggccgcc gaaggagaac catgaaactc tgcagagagc tctcggactt ggtggtgtac
     4141 acaaactccg tggcagccca ggatattgtt gatgatggaa ccacagggaa tgtgttgtcc
     4201 ttcagcgaaa caagagccca tcaagtggtt cagcagaagt cagagcagtt catgatctac
     4261 aaccaaaaac agctcacaag gatttacccc tctgcctacc gcatcgattc cagtaacttc
     4321 aacccactgc cctactggaa tgcaggctgc cagctcgtgg ctctgaacta ccagtctgaa
     4381 gggcgaatga tgcagatcaa tcgagcaaaa ttcaaggcga atggcaactg tggctatatt
     4441 ctcaagcccc agcaaatgtg caaaggtact ttcaaccctt tctctggtga cccacttcct
     4501 gccaacccca aaaaacagct catactgaaa gtgatcagtg gacagcaact ccccaaacct
     4561 cctgactcta tgtttggaga ccgaggcgag atcattgacc ctttcgttga agttgaaatt
     4621 attgggctgc cagtagactg ttgcaaggac caaactcgtg tggtggatga caacggattt
     4681 aaccctgtgt gggaagaaac cctaactttt acagttcaca tgccagaaat agctttggtt
     4741 cggttccttg tgtgggatca tgatcctatt ggaagagact ttgtcggaca aaggactgtg
     4801 accttcagta gcctcgtgcc cggctaccgg catgtctacc tggaaggact cacagaagca
     4861 tctattttcg ttcacataac catcaatgag atctttggaa agtggagtcc tttaatcctc
     4921 aaccccagtt ataccatatt gcactttcta ggagctacaa agaacagaca gctccaaggc
     4981 ctgaaggggc tgttcaataa gaatcccagg cacgcttctt cagaaaacaa ctcgcattat
     5041 gttcggaagc gatcgattgg agataggatt ctgcgacgta cagctagtgc tccagccaaa
     5101 ggcaggaaaa agagcaaagt gggcttccaa gaaatggttg agataaaaga ttctgtctct
     5161 gaggcttcaa gagatcaaga tggtgtcctg aggaggacca cccggagtct gcaagtacgc
     5221 cctgtctcta tgcctgttga caagagcctt ctgggggctt tgtcgctgcc catatctgaa
     5281 gcagcaaaag acacggatgg aaaagaaaac tgtctggcag gagacaagga tgacagaaga
     5341 aagggagccg caactagaaa agacccacat ttttcaaatt tcaacaaaaa gttatcctcc
     5401 tcctccagtg cgctcctcca caaagatgcc aaccaagggc caactgccag tgtatcaaac
     5461 ccagaacagt gtggaggacg aggtgcaaag agtgagagga tcaaaccaaa tatgacaaat
     5521 gattgccagg aaaaccacaa tcccccgaaa ttcctttctc caaggaagca tttggctctg
     5581 gatcctgcaa cgaagggact gcaagagaga ctgcatggaa tgaaaaccaa tgagaaggag
     5641 catgccgagg gcttcttggg agagaaaagc atgctgtccg gaagcgttct ttctcaaagt
     5701 tctctggagg tagagaacct ggaaggcagc agagccaagg gcagagctgc aacctccttt
     5761 tctctctcgg acgtctccgc gctctgttct gacatccctg atttacattc aactgccatt
     5821 ctacaggaca ctgaaatttc caatctcatt gatgacgtga ctttaacaaa tgagaatcag
     5881 tcgggaagtt ccatctcggc actgattggc cagtttgaag aaagcaacca cccggctaac
     5941 gttactgtgg tttctcatct cagcaccagt ggggcatcag gaagtgctcc tttccaaaca
     6001 cccttcaagc atggtctttc ccaggggaac cagaaggcct ccttcttgtg ctcatctcct
     6061 gagctgaata agctctctag tgttgagacc accaaactgg caaataacgc agttccttgt
     6121 ggagtgattg gttctcccat ctctacacca aagccaggtg atgacccttc agataaggcc
     6181 aagacaagag tcattgaagg caacctgcct gggttccctg atgcttctcc tggtcaattt
     6241 cccaaaagcc ccacccatgg agaggaccat agtcaagtga tgaacagccc tgctctctcc
     6301 acggagttgg ccatagaaga tataattgca gaccctgctc tctctataaa ttctgcagaa
     6361 agcagtcttg tggaaattga tggagaatct gaaaacctat ctctaacaac ctgtgactac
     6421 agggaagaag ctccaagtca acttgtttct ccactaaaac tgcagcagag ccaggagatg
     6481 gtggaacaca ttcagagggg cttgaggaat ggctactgta aagagactct cctcccttct
     6541 gaaatattca acaatattcc aggtgtcaaa aatcacagta tttctcatct aacctatcag
     6601 ggtgctggct ttgtgtataa ccatttctca agttcagatg caaaaacgaa ccaaatctgt
     6661 gagccccagc agcctagggc tccagatatg catgccccta cacctacacc atcaacacat
     6721 gctcctttgg ctgctttgaa actgccaagc ccatgcaaat ccaaaagtct gggggactta
     6781 acatcagagg acattgcctg caattttgaa agcaaatatc aatgtattag taggagcttt
     6841 gtgacaaacg gcattaggga caagagcgtg actatgaaga caaagtcatt agagccttta
     6901 gacgccctga ctgaacagct ccggaagctg gtgtcctttg accaagaaga cagctgtcaa
     6961 gtgctctact caaagcagga tgtcaatcag tgtcccaggg cattagtcag aaagttgtcg
     7021 tctagaagtc agagcagagt gcgcaacatt gctagccgtg ccaaggagaa gcaggaagct
     7081 ggcaagcaaa aagctatggc ccagagcacc agaggaggag tggtccttag aagtaaaccg
     7141 cccgctcctg ctctggccgt gaaccgtcac tccacgggct catacattgc aagctacctg
     7201 aggaacatga aagctggtgg cctagagggt cgaggcatcc cagagggagc gtgcacggcc
     7261 cttcgctatg gctacatgga ccagttttgt tcagataatt cagttctgca gactgagcca
     7321 agcagcgaag ataaacccga aatttatttt cttttgaggc ttggatccga ctacaaagac
     7381 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     7441 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     7501 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     7561 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     7621 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     7681 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     7741 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     7801 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     7861 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     7921 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     7981 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     8041 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     8101 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     8161 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     8221 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     8281 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     8341 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     8401 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     8461 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     8521 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     8581 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     8641 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     8701 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     8761 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     8821 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     8881 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     8941 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     9001 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     9061 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     9121 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     9181 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     9241 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     9301 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     9361 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     9421 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     9481 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     9541 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     9601 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     9661 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     9721 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     9781 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     9841 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     9901 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt
     9961 cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca
    10021 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
    10081 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg
    10141 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    10201 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    10261 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
    10321 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
    10381 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10441 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10501 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc
    10561 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
    10621 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa
    10681 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    10741 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    10801 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    10861 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10921 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10981 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    11041 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    11101 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    11161 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    11221 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    11281 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    11341 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    11401 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    11461 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    11521 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    11581 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    11641 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    11701 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    11761 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11821 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11881 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11941 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    12001 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    12061 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    12121 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    12181 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    12241 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    12301 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    12361 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    12421 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    12481 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    12541 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    12601 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    12661 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    12721 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12781 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12841 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    12901 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    12961 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    13021 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
    13081 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    13141 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    13201 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    13261 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
