LOCUS       V032936                 9863 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032936
VERSION     V032936
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(join(9777..9863,1..18))
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     492..508
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     LTR             751..931
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    975..1100
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1593..1826
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             2010..2054
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2315..2432
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2522..2725
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2799..2843
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic
                     ribonuclease A"
                     /transl_table=1
                     /translation="KETAAAKFERQHMDS"
                     /label="S-Tag"
     CDS             2877..2900
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             2904..2927
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             2937..3050
                     /codon_start=1
                     /note="selected from a peptide library; binds streptavidin
                     with nanomolar affinity (Keefe et al., 2001)"
                     /product="streptavidin-binding peptide"
                     /transl_table=1
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
                     /label="SBP"
     CDS             3132..5249
                     /label="Pcca(NM_001425592)"
                     /note="Pcca(NM_001425592)"
                     /gene="Pcca"
     CDS             5256..5279
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             5283..5306
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     promoter        5350..5561
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5574..5842
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5869..6468
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6475..7063
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7137..7370
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7442..7563
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7582..7717
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7755..7771)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7779..7795
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7803..7833)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7848..7869
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8157..8745)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8916..9776)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
       61 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
      121 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
      181 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
      241 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
      301 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
      361 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      421 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      481 gtcacgacgt tgtaaaacga cggccagtgc caagctgacg cgtgtagtct tatgcaatac
      541 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
      601 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
      661 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
      721 tatttaagtg cctagctcga tacaataaac gggtctctct ggttagacca gatctgagcc
      781 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      841 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      901 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga
      961 aagggaaacc agagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     1021 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     1081 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     1141 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     1201 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     1261 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     1321 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1381 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     1441 ccactgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     1501 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     1561 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     1621 gcagcaggaa gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta
     1681 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     1741 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     1801 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     1861 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gattggaatc
     1921 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1981 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     2041 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     2101 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     2161 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2221 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2281 ccattcgatt agtgaacgga tctcgacggt taacttttaa aagaaaaggg gggattgggg
     2341 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2401 tacaaaaaca aattacaaaa attcaaaatt ttatcgatac tagtattatg cccagtacat
     2461 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     2521 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     2581 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     2641 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     2701 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     2761 tccacgctgt tttgacctcc atagaagatt ctagaatgaa agaaaccgct gctgctaaat
     2821 tcgaacgcca gcacatggac agcggagccg gcgcaggagc cggcgctgac gcgcctgact
     2881 acaaagacga tgacgacaag ggagattaca aggatgacga tgacaaaggc gcgtcgatgg
     2941 acgagaagac caccggctgg cggggcggcc acgtggtgga gggcctggcc ggcgagctgg
     3001 agcagctgcg ggccaggctg gagcaccacc ctcagggcca gcgggagccc tccggcggct
     3061 gcaagctggg cggcgcgcct gaattctgca gtcgacggta ccgcgggccc atcgctcgat
     3121 ctagagctag catggcgggg cagtgggtca ggaccgtggc gctgttggcg gccaggcggc
     3181 attggcggcg gtcctcgcag cagcaattgc tggggacgct gaagcatgct ccagtctatt
     3241 cataccaatg cctagtggtg tccagaagtc tcagttctgt ggaatatgag cctaaagaaa
     3301 agacttttga taaaattctc attgctaaca gaggagaaat tgcctgtagg gttattaaaa
     3361 cttgcaagaa gatgggcatc aagacagttg ccattcacag tgatgttgat gccagttctg
     3421 ttcacgtgaa aatggcggat gaggctgtct gtgttggccc agctcccacc agtaaaagct
     3481 acctcaacat ggatgccatc atggaagcca ttaagaaaac cagggcccaa gctgtacacc
     3541 cagggtatgg attcctgtca gaaaacaaag agtttgcaaa gcgtctggca gcagaagatg
     3601 tcactttcat tggacctgat actcatgcta ttcaagccat gggtgacaag atagaaagca
     3661 aactattagc caagagagca aaggtcaaca caatccctgg ttttgatggg gtagtaaagg
     3721 atgcagatga agctgtcaga attgcaaggg aaattggcta ccctgtgatg atcaaggcct
     3781 cagcaggcgg tggtgggaaa ggcatgcgca tcgcctggga tgacgaagag accagggatg
     3841 gctttagatt ttcatcccag gaagctgctt ctagttttgg tgatgataga ctactaatag
     3901 aaaaatttat cgataaccct cgtcatatag aaatccaggt tttaggggat aaacatggca
     3961 atgctctgtg gctcaatgag agggagtgct cgatccagag aaggaaccag aaggtggtag
     4021 aggaggcgcc aagcattttt ctggatcctg aaactcgcca agcaatggga gagcaggccg
     4081 tggctttggc taaagccgtg aagtattctt ctgctggaac tgtggaattt cttgtggact
     4141 cccagaagaa tttttacttc ttggagatga atacaagact acaggtcgaa catcctgtca
     4201 cagagtgcat tactggcctg gacttagtcc aagaaatgat ccttgttgct aagggttacc
     4261 cactcaggca caagcaagag gatattccca tcagtggctg ggcagttgaa tgtcgggttt
     4321 atgctgagga cccctacaag tctttcggtt taccgtctat tgggaggctg tcccagtacc
     4381 aagagccgat acatctacct ggtgtccgag ttgacagtgg catccaacca ggaagtgaca
     4441 tcagcatcta ttatgatcct atgatttcaa agctagtcac atatgggtct gacagagcag
     4501 aagccctgaa gaggatggaa gacgcactgg acaattatgt gatccggggt gttacacaca
     4561 acatcccatt gctccgggag gtgataatca acacacgttt tgtgaaagga gacatcagca
     4621 ctaagtttct ctctgatgtg tatcctgatg gcttcaaagg gcacacgtta acactgagtg
     4681 agagaaacca gttattggcc attgcatcat ctgtatttgt ggcatcccag ctacgagctc
     4741 agcgcttcca agaacattca agagtaccag ttattaggcc tgatgtggct aagtgggagc
     4801 tctcggtaaa gttacatgat gaagatcata ctgtcgtggc atctaacaat gggccggcat
     4861 ttaccgtgga agttgatggc tcgaaactaa atgtgaccag tacgtggaac ctggcgtcac
     4921 ccttattgtc tgtcaacgtt gatggcacgc agaggactgt gcagtacaaa gtgcacattt
     4981 taaccaagct tgctgcagag ctgaacaaat tcatgcttga aaaagtgccc aaggacacca
     5041 gcagcactct gtgctccccg atgcctggag tggtggtggc cgtttctgtc aagcctggag
     5101 acatggtagc agaaggtcag gaaatctgtg tgattgaagc tatgaaaatg cagaacagta
     5161 tgacagctgg gaaaatgggc aaggtgaaat tggtgcactg caaagctgga gacacagttg
     5221 gtgaaggaga cctgcttgtg gagctggaag gatccgatta taaggatgat gacgacaaag
     5281 gagactataa agacgacgat gacaagtagc ggccgcgaag gatctgcgat cgctccggtg
     5341 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     5401 gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     5461 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     5521 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc
     5581 gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc
     5641 gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt
     5701 taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca
     5761 gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt
     5821 ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagat gaccgagtac
     5881 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     5941 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6001 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6061 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6121 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6181 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6241 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6301 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6361 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6421 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt cgacaatcaa
     6481 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6541 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6601 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6661 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6721 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6781 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6841 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6901 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6961 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7021 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     7081 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7141 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg
     7201 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7261 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7321 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7381 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7441 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7501 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7561 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg
     7621 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7681 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca
     7741 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7801 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7861 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7921 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7981 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8041 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8101 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8161 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8221 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8281 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8341 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8401 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8461 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8521 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8581 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8641 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8701 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8761 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8821 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8881 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8941 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9001 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9061 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9121 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9181 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9241 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9301 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9361 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9421 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9481 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9541 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9601 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9661 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9721 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9781 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9841 tttgaatgta tttagaaaaa taa
//
