LOCUS       V041121                13512 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041121
VERSION     V041121
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2285..3934
                     /EC_number=""
                     /codon_start=1
                     /experiment=""
                     /gene="luc"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
                     /label="luciferase"
     CDS             3971..8365
                     /label="PTPRM(NM_001105244)"
                     /note="PTPRM(NM_001105244)"
                     /gene="PTPRM"
     CDS             8372..8437
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8482..8693
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8706..8974
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             9001..9600
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9607..10195
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10269..10502
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10574..10695
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10714..10849
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10887..10903)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10911..10927
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10935..10965)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10980..11001
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11289..11877)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12048..12908)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12909..13013)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13487..13503
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattgc
     2281 caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctagagga
     2341 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
     2401 aattgctttt acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat
     2461 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
     2521 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
     2581 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa
     2641 catttcgcag cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
     2701 gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca
     2761 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
     2821 cgattttgta ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaactcctc
     2881 tggatctact gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt
     2941 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
     3001 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
     3061 atttcgagtc gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga
     3121 ttacaaaatt caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac
     3181 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct
     3241 ttcgaaagaa gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg
     3301 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
     3361 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
     3421 gaaaacgctg ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc
     3481 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
     3541 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa
     3601 gtctttaatt aaatacaaag gataccaggt ggcccccgct gaattggagt cgatattgtt
     3661 acaacacccc aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga
     3721 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
     3781 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
     3841 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
     3901 cataaaggcc aagaagggcg gaaagtccaa attgggtgga ggcggatcag gaggcggtgg
     3961 atctgaattc atgaggggac ttgggacttg cctggcgact ttggccggac ttttgctaac
     4021 tgcggcgggc gagacgttct caggtggctg cctctttgat gagccgtata gcacatgtgg
     4081 atatagtcaa tctgaaggtg atgacttcaa ttgggagcaa gtgaacacct tgactaaacc
     4141 gacttctgat ccatggatgc catcaggttc tttcatgctg gtgaatgcct ctgggagacc
     4201 tgaggggcag agagcccacc tgctcttacc ccaacttaaa gaaaatgaca cccactgcat
     4261 cgattttcac tattttgtgt ccagcaagag taattctcct ccggggttac tcaatgtcta
     4321 cgtgaaggtc aataacgggc cactggggaa tcctatctgg aatatatctg gagacccaac
     4381 acgtacatgg aacagggcag aactggccat tagtactttc tggcctaact tttatcaggt
     4441 gatttttgaa gtgataactt ctggacatca aggctatctc gctatcgatg aggtgaaggt
     4501 gttaggacat ccatgtacca ggactcctca cttcctgcgg attcagaatg tggaagttaa
     4561 tgctggccag tttgctacct tccagtgcag tgccatcggc aggaccgtgg caggagacag
     4621 gctctggtta cagggcattg atgtgcgaga tgctcctctg aaggaaatca aggtgaccag
     4681 ctcccgacgc ttcattgctt catttaatgt tgtgaatacc accaaacgag atgctggaaa
     4741 gtaccgctgc atgattcgca ctgaaggagg tgttggaata tcaaactatg cagagttggt
     4801 agttaaagaa ccacccgttc ctattgcccc acctcagctc gcctctgtag gagccaccta
     4861 cctgtggata cagctcaacg ccaactccat caatggggat gggcccattg tggcccgaga
     4921 ggtggagtac tgcacggcca gtgggagctg gaatgaccgg cagccagtcg attccacgag
     4981 ctataaaatt ggacaccttg acccagatac agaatatgag attagtgtgc tcctgaccag
     5041 gccaggggag ggtggcactg gctctcctgg tccagctctc aggacaagaa caaagtgtgc
     5101 tgatcccatg cgaggcccaa gaaaactaga agtagtggag gtcaaatctc ggcaaatcac
     5161 tatccgctgg gagccatttg gatataatgt aactcgttgc cacagttata atctcactgt
     5221 ccactactgt taccaagttg gaggacaaga acaagtgcga gaagaagtaa gctgggatac
     5281 agaaaactca caccctcaac acacgatcac taacctgtca ccatacacca atgtcagtgt
     5341 gaaactgatc ctcatgaacc cagagggccg gaaggaaagc caagaactca tagtgcagac
     5401 agatgaagac ctcccaggtg ctgttcccac tgaatccata caaggaagta cctttgaaga
     5461 gaagatattt cttcagtgga gagaaccaac tcaaacatat ggtgtaatca ctttatatga
     5521 gatcacctac aaagcagtca gttcctttga cccagaaata gatttatcca atcagagtgg
     5581 aagagtttca aagctgggaa atgaaaccca ttttctgttt tttggactgt atccggggac
     5641 cacatactcc tttaccatcc gagctagcac agctaagggt tttgggcctc cagcaacaaa
     5701 ccagttcacc accaaaatat cagcaccctc tatgccagct tatgaacttg agacaccttt
     5761 gaatcaaact gacaataccg tgacagtcat gctgaaacct gcccacagca gaggagcacc
     5821 tgtcagtgtc tatcaaatag ttgttgagga agaacgtcct cgaagaacta aaaagacgac
     5881 agaaatctta aagtgctacc cagtgccaat tcacttccag aatgcttctc tgctgaactc
     5941 acagtactac tttgctgcag aatttcctgc agacagcctc caagctgcgc agccttttac
     6001 aattggtgat aataagacat ataatggata ctggaacact ccccttctcc cctataaaag
     6061 ctacagaatt tatttccaag ctgctagtag agccaatggg gaaaccaaaa tagactgtgt
     6121 ccaagtggcc acaaaaggag ctgccactcc gaaaccagtc ccagaacccg agaaacagac
     6181 agaccataca gttaaaattg ctggagtcat cgcgggcatc ttgctgttcg tgattatatt
     6241 tcttggagtt gtgttggtaa tgaagaaaag gaaactggcc aagaagcgga aagagaccat
     6301 gagcagcacc cgacaggaga tgactgtgat ggtgaactca atggacaaga gctatgctga
     6361 gcagggcaca aactgcgacg aggctttctc attcatggac acgcacaatc tgaatgggag
     6421 atctgtgtct tcaccatcgt ccttcacaat gaaaacaaat acactgagca catcggtgcc
     6481 taattcctat tacccagacc catttgtgcc aactgcaatt ttagtgccaa taaatgatga
     6541 aacccacaca atggccagcg ataccagcag cctggtgcag tcccatactt acaagaagcg
     6601 agagccggcc gacgtgccct atcagactgg gcagctccac cccgccatcc gggtggcaga
     6661 cctccttcag cacatcacac agatgaagtg tgcggagggc tacggcttca aggaggaata
     6721 cgagagcttc tttgaagggc agtctgcacc atgggactcg gctaagaaag atgagaacag
     6781 aatgaagaac agatacggga atatcattgc atacgatcat tcccgagtga ggctgcagac
     6841 aatagaagga gacacaaact cagactatat caatggcaat tatatcgatg gttatcatcg
     6901 acccaatcat tacattgcta cccaagggcc aatgcaggaa accatctatg acttctggag
     6961 gatggtgtgg cacgaaaaca ctgcaagtat catcatggtg accaatcttg tggaagtggg
     7021 aagggtcaaa tgctgcaaat actggccaga tgacacagag atatataaag acattaaagt
     7081 taccctaata gaaacagaac tactggcaga atatgtgata agaacatttg ctgttgaaaa
     7141 gagaggtgtg catgaaatcc gagagatcag acagtttcac ttcactggct ggccggatca
     7201 tggggtcccc taccatgcca ccggcctgct gggattcgtg cggcaagtca agtccaagag
     7261 cccgcccagt gcaggcccac tggtggtgca ctgcagtgct ggtgcaggga ggactggctg
     7321 tttcatcgtc attgatatca tgttggacat ggccgaaagg gaaggggtcg tagacatcta
     7381 caactgcgtc agggagctgc ggtcacggag ggtgaacatg gtgcaaacag aggagcagta
     7441 tgtgtttatc cacgatgcga tcctggaagc ctgtctttgt ggggacacct ctgtgcctgc
     7501 ttcccaagtt aggtctctgt attatgacat gaacaaactg gatccacaga caaactcaag
     7561 ccagattaaa gaggaattcc ggacgctaaa catggtgaca ccaacgctgc gagtagagga
     7621 ctgcagcatc gcactgttgc cccggaacca tgagaaaaac cggtgcatgg acatcctgcc
     7681 cccagaccgc tgcctgccct tcctcatcac catcgatggg gagagcagca actacatcaa
     7741 tgctgccctc atggacagct ataaacagcc ttcagctttt atagtcaccc agcatccttt
     7801 gccaaacaca gtgaaagact tttggagact ggtcctggat tatcactgca catccgtagt
     7861 tatgctaaat gatgtggatc ctgcccagtt gtgtccacag tactggccag aaaacggagt
     7921 acacagacac ggccccatcc aggtggaatt tgtctctgct gacctggaag aggacatcat
     7981 cagcaggata ttccgcattt acaatgccgc cagaccccaa gatggatatc ggatggtgca
     8041 gcaattccag ttcctgggct ggccgatgta cagggacaca ccagtgtcta agcgctcctt
     8101 cttgaagctc attcgccagg tggacaagtg gcaagaggag tacaatggcg gggaaggccg
     8161 cacggttgtg cactgcttga acgggggagg ccgcagtggg acgttctgcg ccatcagcat
     8221 cgtatgtgag atgctccggc accagagaac cgtggatgtc tttcacgctg tgaagacact
     8281 gaggaacaac aagcccaaca tggtcgacct cctggatcag tacaagttct gctacgaggt
     8341 ggccctggaa tacttgaatt ctggcggatc cgactacaaa gaccatgacg gtgattataa
     8401 agatcatgac atcgactaca aggatgacga tgacaagtga gcggccgcga aggatctgcg
     8461 atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     8521 ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa
     8581 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     8641 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc
     8701 ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac
     8761 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg
     8821 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc
     8881 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct
     8941 ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag
     9001 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     9061 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
     9121 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     9181 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     9241 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     9301 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     9361 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     9421 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     9481 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     9541 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     9601 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     9661 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     9721 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     9781 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     9841 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     9901 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     9961 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
    10021 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    10081 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    10141 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
    10201 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
    10261 ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac
    10321 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    10381 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    10441 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    10501 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
    10561 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10621 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10681 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc
    10741 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    10801 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
    10861 agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    10921 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    10981 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    11041 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    11101 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    11161 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    11221 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    11281 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    11341 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    11401 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    11461 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    11521 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    11581 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    11641 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    11701 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
    11761 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    11821 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    11881 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    11941 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    12001 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    12061 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    12121 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    12181 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    12241 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    12301 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    12361 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    12421 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    12481 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    12541 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    12601 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    12661 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    12721 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    12781 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    12841 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    12901 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    12961 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    13021 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    13081 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
    13141 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    13201 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
    13261 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
    13321 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
    13381 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
    13441 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
    13501 agtgccaagc tg
//
