LOCUS       V042836                10987 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042836
VERSION     V042836
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5013
                     /label="PRRT4(NM_001174164)"
                     /note="PRRT4(NM_001174164)"
                     /gene="PRRT4"
     CDS             5020..5085
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5129..5340
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5353..5621
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5650..5659
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5680..6342
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6412..6465
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6466..7065
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7066..7654
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7728..7961
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8033..8154
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8194..8329
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8362..8378)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8386..8402
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8410..8440)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8455..8476
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8764..9352)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9523..10383)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10384..10488)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10962..10978
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ccaggcatgg ctgtctaggg
     2341 ctgggactgt tctgctgcgt cctgtttgct gctactgtgg gcccccagcc caccccctcc
     2401 atcccaggtg cccctgccac cactttgacc cccgtacctc aaagtgaggc ctctatgctg
     2461 tctctcaacc tgggacttaa cttcaaattc catcttcggg gacctgctgc tgtctggggg
     2521 agcccagtca cagagaccca gccactctct cttgggccag gccaggagcc aggggaagag
     2581 gtggccagtg ggctgaggac tgaccccctt tgggaattgc tggtgggctc ctcagggaac
     2641 tctctcactg agtggggctc caccgaaggt ggctcaaagc cccgggcctc ctccctgctt
     2701 ccggagtcca catcccggcg ctctgggccc agcgatgggc ccactgcccc ctatcagccc
     2761 aggaggagca ctgtgacctg ggacactgct ctgatggtga cagcacttcc atccagtgct
     2821 cccaggcccc accagagcga gctggagctg aagtttgaca tggcactgag agcaggtgca
     2881 gcccccacgc ttgggcatcg aacgctgccc ctgctgccca gcctgcgggc cagcctggca
     2941 gagattgctg ggcgcctggg accctttgga ttctttggca ctactctgtc cccactccgg
     3001 aacttctccg gcctgagccc cccaggtgaa actacatcca caagctctgc ctctggagtt
     3061 tcgggttctc tggggttcct tggtaccact ctgtccctgc ccccatactc cctggagagg
     3121 aagctctcca gcccaagtcc tctggaccca gctgcttccc taagttttgc ctcgattgca
     3181 acaacatcat tagaccccac agtccccatc tctggcccag atgacctctc tcctcccgcc
     3241 agcctcggga acccttcggg gcagccagag tgtgggccag ggtcctgcag cgtgggagaa
     3301 ttgcctgaac gcgaggggca gcctcccgag gcgccgaggc ccctcttttt cctgaccctg
     3361 gaggccgact gggcagaggc cagggctcgc tgggggctgg cctgggaggc ccacgtgtac
     3421 ggggtaggcg cgctcttcgg cctggttgcc ttgctggcgc tgctggctct ggccctcttg
     3481 ccctggcggt gcccgcccgg cgccccctgc ctggcgctgc tggacctgct gctgctgtcg
     3541 gccgggacca cgcgggcctt cccgctcttc tacgacgcct atgggcacag ggatcgactg
     3601 cccgcgctcg cctggctgct gctgcaggac cttccgctgc cctgcttggc tgccggcctg
     3661 gggctggcct gcctgctgct ggcccggccg cgcccgccgc ggtgccccac cggcctggct
     3721 gcgctgctgc tcctggggct ggggctggcg gccgccgccg ccctcgggag cgccgcgcat
     3781 cgcccgctgc ggcccctgcg gctcgcctcg cgcgggctgc acgcctttct cgccgctttc
     3841 ctttccgggc tgctgctggc gctctcctgc tggggcgggc ggcggcggcg ggccggggcg
     3901 cccctgggag ggtcgggctt caagggcgcc acacccctgc cgcaggggcg cagccccttc
     3961 gcccctcggg agtcctggcg gcgcgcggcg cgcacggccc cggtggcggg caccttcggg
     4021 ctgctgagcg gagccctgca gggctatgag gtgctgcacg ccctgggcta cggcggccaa
     4081 tccggcctgg aagggccctg gccctggtgg gccttccagc taggcctgcg cctgggcgag
     4141 gtgggcgtcg cgctcccgtt ggcgctgctg ggcctctacc cggcgctctg cagtcctcgc
     4201 gtgccgccgc gctgctgggc caagctcttc cgcttgtccc cgggccacgc ggctccgctg
     4261 ctgccgggag gctgggtcac tgggccccca gacaaggagc ccctggggag cgccatcgct
     4321 cgcggggacg cggagctgct gcagctgtgc gcgctggcag ggccaggccc agacctccta
     4381 ctccagggcg gaggttgccg gggcttcgaa ggcgcggcgg ccaacccggc cccgtccccg
     4441 gcttcctctc cctgcagcga ttacaccgtg gacttccgcc cgccctcccc aatcaacctg
     4501 cgacgcagca tcgaggaggc cctctgcagc gaggccctgc ttgcgcctgg cctcttccag
     4561 ggccctgcct tcgaggacgc tctgcctggg ctcggactct accgcaccgc ctcgctgggg
     4621 accgggggca gggccagtga gagatcaggg gaggcctctg gccccgctgc gcccccggag
     4681 ctcccctccc ctggggcttg gcccgcaggc agcagcgtct catctggctc gttctgcgga
     4741 ctctcgcggg acagctcgtc catgctgctg tgttccagcc ccgacaggcc cccgcgctgc
     4801 cctctggtct gcgtcctcag tcccccgcgg ccctcaggaa gcagccccag cctcccggcc
     4861 tcaggatcct accaggccct gtccccaccc tctcgcgact ccccagagcc tgcttctgag
     4921 ctgcaggccg aggaggcctt gctgcaggag cagttcctgg acgcctgccg acagatcgac
     4981 gagctgagcg tgggcagcga caccatagac ctgggatccg actacaaaga ccatgacggt
     5041 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     5101 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5161 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5221 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5281 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5341 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5401 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5461 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5521 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5581 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     5641 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     5701 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     5761 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5821 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     5881 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     5941 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     6001 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     6061 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     6121 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     6181 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     6241 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     6301 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6361 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6421 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     6481 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6541 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6601 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6661 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6721 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6781 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6841 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6901 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6961 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7021 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     7081 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7141 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7201 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7261 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7321 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7381 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7441 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7501 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7561 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7621 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     7681 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7741 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     7801 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7861 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7921 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7981 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8041 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8101 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8161 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8221 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8281 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     8341 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8401 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8461 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8521 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8581 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8641 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8701 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8761 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8821 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8881 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8941 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9001 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9061 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9121 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9181 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     9241 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9301 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9361 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9421 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9481 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9541 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9601 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9661 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9721 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9781 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9841 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9901 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9961 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10021 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10081 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10141 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10201 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10261 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10321 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10381 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10441 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10501 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10561 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    10621 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    10681 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    10741 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    10801 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    10861 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    10921 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    10981 caagctg
//
