LOCUS V037439 11045 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037439 VERSION V037439 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..5139 /label="PRPF6(NM_012469)" /note="PRPF6(NM_012469)" /gene="PRPF6" promoter 5204..5415 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5428..5696 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5725..5734 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5755..6417 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 6487..6540 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6541..7140 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7141..7728 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7802..8035 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 8107..8228 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 8247..8382 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(8420..8436) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8444..8460 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8468..8498) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8513..8534 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8822..9410) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9581..10441) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10442..10546) /gene="bla" /label="AmpR promoter" primer_bind 11020..11036 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga acaagaagaa gaaaccgttc 2341 ctagggatgc ccgcgcccct cggctacgtg ccggggctgg gccggggcgc cactggcttc 2401 accacgcggt cagacattgg gcccgcccgt gatgcaaatg accctgtgga tgatcgccat 2461 gcacccccag gcaagagaac cgttggggac cagatgaaga aaaatcaggc tgctgacgat 2521 gacgacgagg atctaaatga caccaattac gatgagttta atggctatgc tgggagcctc 2581 ttctcaagtg gaccctacga gaaagatgat gaggaagcag atgctatcta tgcagccctg 2641 gataaaagga tggatgaaag aagaaaagaa agacgggagc aaagggagaa agaagaaata 2701 gagaaatatc gtatggaacg ccccaaaatc caacagcagt tctcagacct caagaggaag 2761 ttggcagaag tcacagaaga agagtggctg agcatccccg aggttggcga tgccagaaat 2821 aaacgtcagc ggaacccacg ctatgagaag ctgacccctg ttcctgacag tttctttgcc 2881 aaacatttac agaccggaga gaaccatacc tcagtggatc cccgacaaac tcaatttgga 2941 ggtcttaaca caccctatcc aggtggacta aacactccat acccaggtgg aatgacgcca 3001 ggactgatga cacctggcac aggtgagctg gacatgagga agattggcca agcgaggaac 3061 actctgatgg acatgaggct gagccaggtg tctgactccg tgagtggaca gaccgtcgtt 3121 gaccccaaag gctacctgac ggatttaaat tccatgatcc cgacacacgg aggagacatc 3181 aatgatatca agaaggcgcg actgctcctc aagtctgttc gggagacgaa ccctcatcac 3241 ccgccagcct ggattgcatc agcccgcctg gaagaagtca ctgggaagct acaagtagct 3301 cggaacctta tcatgaaggg gacggagatg tgccccaaga gtgaagatgt ctggctggaa 3361 gcagccaggt tgcagcctgg ggacacagcc aaggccgtgg tagcccaagc tgtccgtcat 3421 ctcccacagt ctgtcaggat ttacatcaga gccgcagagc tggaaacgga cattcgtgca 3481 aagaagcggg ttcttcggaa agccctcgag catgttccaa actcggttcg cttgtggaaa 3541 gcagccgttg agctggaaga acctgaagat gctagaatca tgctgagccg agctgtggag 3601 tgctgcccca ccagcgtgga gctctggctt gctctggcaa ggctggagac ctatgaaaat 3661 gcccgcaagg tcttgaacaa ggcgcgggag aacattccta cagaccgaca tatctggatc 3721 acggctgcta agctggagga agccaatggg aacacgcaga tggtggagaa gatcatcgac 3781 cgagccatca cctcgctgcg ggccaacggt gtggagatca accgtgagca gtggatccag 3841 gatgccgagg aatgtgacag ggctgggagt gtggccacct gccaggccgt catgcgtgcc 3901 gtgattggga ttgggattga ggaggaagat cggaagcata cctggatgga ggatgctgac 3961 agttgtgtag cccacaatgc cctggagtgt gcacgagcca tctacgccta cgccctgcag 4021 gtgttcccca gcaagaagag tgtgtggctg cgcgccgcgt acttcgagaa gaaccatggc 4081 actcgggagt ccctggaagc actcctgcag agggctgtgg cccactgccc caaagcagag 4141 gtgctgtggc tcatgggcgc caagtccaag tggctggcag gggatgtgcc tgcagcaagg 4201 agcatcctgg ccctggcctt ccaggccaac cccaacagtg aggagatctg gctggcagcc 4261 gtgaagctgg agtccgagaa tgatgagtac gagcgggccc ggaggctgct ggccaaggcg 4321 cggagcagtg cccccaccgc ccgggtgttc atgaagtctg tgaagctgga gtgggtgcaa 4381 gacaacatca gggcagccca agatctgtgc gaggaggccc tgcggcacta tgaggacttc 4441 cccaagctgt ggatgatgaa ggggcagatc gaggagcaga aggagatgat ggagaaggcg 4501 cgggaagcct ataaccaggg gttgaagaag tgtccccact ccacacccct gtggcttttg 4561 ctctctcggc tggaggagaa gattgggcag cttactcgag cacgggccat tttggaaaag 4621 tctcgtctga agaacccaaa gaaccctggg ctgtggttgg agtccgtgcg gctggagtac 4681 cgtgcggggc tgaagaacat cgcaaataca ctcatggcca aggcgctgca ggagtgcccc 4741 aactccggta tcctgtggtc tgaggccatc ttcctcgagg caaggcccca gaggaggacc 4801 aagagcgtgg atgccctgaa gaagtgtgag catgaccccc atgtgctcct ggccgtggcc 4861 aagctgtttt ggagtcagcg gaagatcacc aaggccaggg agtggttcca ccgcactgtg 4921 aagattgact cggacctggg ggatgcctgg gccttcttct acaagtttga gctgcagcat 4981 ggcactgagg agcagcagga ggaggtgagg aagcgctgtg agagtgcaga gcctcggcat 5041 ggggagctgt ggtgcgccgt gtccaaggac atcgccaact ggcagaagaa gatcggggac 5101 atccttaggc tggtggccgg ccgcatcaag aacaccttct gaggatccct gcgatatcca 5161 gcgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 5221 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 5281 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 5341 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 5401 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 5461 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 5521 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 5581 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 5641 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 5701 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 5761 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 5821 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 5881 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 5941 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 6001 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 6061 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 6121 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 6181 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 6241 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 6301 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 6361 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 6421 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 6481 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 6541 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 6601 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 6661 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 6721 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 6781 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 6841 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 6901 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 6961 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 7021 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 7081 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 7141 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 7201 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 7261 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 7321 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 7381 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 7441 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 7501 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 7561 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 7621 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 7681 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 7741 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 7801 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 7861 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 7921 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 7981 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 8041 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 8101 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 8161 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 8221 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 8281 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 8341 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 8401 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 8461 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 8521 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 8581 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 8641 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 8701 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 8761 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 8821 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 8881 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 8941 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 9001 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 9061 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 9121 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 9181 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 9241 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 9301 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 9361 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 9421 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 9481 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 9541 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 9601 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 9661 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 9721 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 9781 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 9841 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 9901 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 9961 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 10021 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 10081 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 10141 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 10201 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 10261 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 10321 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 10381 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 10441 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 10501 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 10561 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 10621 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 10681 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 10741 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 10801 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 10861 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 10921 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 10981 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 11041 agctg //