LOCUS       V034593                10197 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034593
VERSION     V034593
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4392
                     /label="PRMT7(NM_019023)"
                     /note="PRMT7(NM_019023)"
                     /gene="PRMT7"
     CDS             4399..4425
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4429..4455
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4459..4485
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4550..4761
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4774..5042
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5071..5080
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5101..5763
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5833..5886
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5887..6285
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    6292..6880
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6954..7187
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7259..7380
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7399..7534
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7572..7588)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7596..7612
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7620..7650)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7665..7686
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7974..8562)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8733..9593)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9594..9698)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10172..10188
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga agatcttctg cagtcgggcc
     2341 aatccgacca cggggtctgt ggagtggctg gaggaggatg aacactatga ttaccaccag
     2401 gagattgcaa ggtcatctta tgcagatatg ctacatgaca aagacagaaa tgtaaaatac
     2461 taccaaggta tccgggctgc cgtgagcagg gtgaaggaca gaggacagaa ggccttggtt
     2521 ctcgacattg gcactggcac gggactcttg tcaatgatgg cggtcacagc aggtgccgac
     2581 ttctgctatg ccatcgaggt tttcaagcct atggctgatg ctgctgtgaa gattgtggag
     2641 aaaaatggct ttagtgataa gattaaggtt atcaacaagc attccaccga ggtgactgta
     2701 ggtccagagg gtgacatgcc atgccgtgcc aacatcctgg tcacagagtt gtttgacaca
     2761 gagctgatcg gggagggggc gctgccctcc tatgagcacg cacacaggca tctcgtggag
     2821 gaaaattgtg aggccgtgcc ccacagagcc accgtctatg cacagctggt ggagtccggg
     2881 aggatgtggt cgtggaacaa gctatttccc atccacgtgc agaccagcct cggagagcag
     2941 gtcatcgtcc ctcccgttga cgtggagagc tgccctggcg caccctctgt ctgtgacatt
     3001 cagctgaacc aggtgtcacc agccgacttt acagtcctca gcgatgtgct gcccatgttc
     3061 agcatagact tcagcaagca agtcagtagc tcagcagcct gccatagcag gcggtttgaa
     3121 cctctgacat ctggccgagc tcaggtggtt ctctcgtggt gggacattga aatggaccct
     3181 gaggggaaga tcaagtgcac catggccccc ttctgggcac actcagaccc agaggagatg
     3241 cagtggcggg accactggat gcagtgtgtg tacttcctgc cacaagagga gcctgtggtg
     3301 cagggctcag cgctctatct ggtagcccac cacgatgact actgcgtatg gtacagcctg
     3361 cagaggacca gccctgaaaa gaatgagaga gtccgccaga tgcgccccgt gtgtgactgc
     3421 caggctcacc tgctctggaa ccggcctcgg tttggagaga tcaatgacca ggacagaact
     3481 gatcgatacg tccaggctct gaggaccgtg ctgaagccag acagcgtgtg cctgtgtgtc
     3541 agcgatggca gcctgctctc cgtgctggcc catcacctgg gggtggagca ggtgtttaca
     3601 gtcgagagtt cagcagcttc tcacaaactg ttgagaaaaa tcttcaaggc taaccacttg
     3661 gaagataaaa ttaacatcat agagaaacgg ccggaattat taacaaatga ggacctacag
     3721 ggcagaaagg tctctctcct cctgggcgag ccgttcttca ctaccagcct gctgccgtgg
     3781 cacaacctct acttctggta cgtgcggacc gctgtggacc agcacctggg gccaggtgcc
     3841 atggtgatgc cccaggcagc ctcgctgcac gctgtggttg tggagttcag ggacctgtgg
     3901 cggatccgga gcccctgtgg tgactgcgaa ggcttcgacg tgcacatcat ggacgacatg
     3961 attaagcgtg ccctggactt cagggagagc agggaagctg agccccaccc gctgtgggag
     4021 tacccatgcc gcagcctctc cgagccctgg cagatcctga cctttgactt ccagcagccg
     4081 gtgcccctgc agcccctgtg tgccgagggc accgtggagc tcagaaggcc cgggcagagc
     4141 cacgcagcgg tgctatggat ggagtaccac ctgaccccgg agtgcacgct cagcactggc
     4201 ctcctggagc ctgcagaccc cgaggggggc tgctgctgga acccccactg caagcaggcc
     4261 gtctacttct tcagccctgc cccagatccc agagcactgc tgggtggccc acggactgtc
     4321 agctatgcag tggagtttca ccccgacaca ggcgacatca tcatggagtt caggcatgca
     4381 gataccccag acctcgagta cccatacgac gtacctgatt atgcaactta tccttatgat
     4441 gtacctgatt atgctagcta tccttatgat gtcccagact acgcataggg atccctgcga
     4501 tatccagcgc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     4561 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     4621 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4681 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4741 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     4801 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     4861 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     4921 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     4981 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     5041 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     5101 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     5161 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     5221 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     5281 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     5341 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     5401 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     5461 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     5521 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     5581 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     5641 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     5701 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     5761 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     5821 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     5881 ggccctatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     5941 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     6001 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     6061 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     6121 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     6181 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     6241 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagtcga caatcaacct
     6301 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6361 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6421 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6481 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6541 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6601 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6661 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6721 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6781 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6841 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     6901 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6961 gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt
     7021 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7081 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7141 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7201 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     7261 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7321 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7381 tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc
     7441 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7501 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat
     7561 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7621 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7681 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7741 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7801 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7861 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7921 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7981 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8041 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8101 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8161 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8221 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8281 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8341 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8401 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8461 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8521 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8581 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8641 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8701 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8761 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8821 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8881 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8941 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9001 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9061 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9121 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9181 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9241 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9301 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9361 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9421 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9481 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9541 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9601 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9661 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9721 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     9781 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     9841 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     9901 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     9961 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    10021 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
    10081 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
    10141 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
