LOCUS       V037384                 9752 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037384
VERSION     V037384
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..2955
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
                     /label="GST"
     CDS             2995..4671
                     /label="PRKAA1(NM_006251)"
                     /note="PRKAA1(NM_006251)"
                     /gene="PRKAA1"
     promoter        4722..4933
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4946..5214
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5241..5840
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5847..6435
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6509..6742
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6814..6935
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6954..7089
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7127..7143)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7151..7167
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7175..7205)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7220..7241
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7529..8117)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8288..9148)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9149..9253)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9727..9743
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtcccct atactaggtt attggaaaat taagggcctt
     2341 gtgcaaccca ctcgacttct tttggaatat cttgaagaaa aatatgaaga gcatttgtat
     2401 gagcgcgatg aaggtgataa atggcgaaac aaaaagtttg aattgggttt ggagtttccc
     2461 aatcttcctt attatattga tggtgatgtt aaattaacac agtctatggc catcatacgt
     2521 tatatagctg acaagcacaa catgttgggt ggttgtccaa aagagcgtgc agagatttca
     2581 atgcttgaag gagcggtttt ggatattaga tacggtgttt cgagaattgc atatagtaaa
     2641 gactttgaaa ctctcaaagt tgattttctt agcaagctac ctgaaatgct gaaaatgttc
     2701 gaagatcgtt tatgtcataa aacatattta aatggtgatc atgtaaccca tcctgacttc
     2761 atgttgtatg acgctcttga tgttgtttta tacatggacc caatgtgcct ggatgcgttc
     2821 ccaaaattag tttgttttaa aaaacgtatt gaagctatcc cacaaattga taagtacttg
     2881 aaatccagca agtatatagc atggcctttg cagggctggc aagccacgtt tggtggtggc
     2941 gaccatcctc caaaaggtgg aggcggatca ggaggcggtg gatctcgcgg ccgcatgcgc
     3001 agactcagtt cctggagaaa gatggcgaca gccgagaagc agaaacacga cgggcgggtg
     3061 aagatcggcc actacattct gggtgacacg ctgggggtcg gcaccttcgg caaagtgaag
     3121 gttggcaaac atgaattgac tgggcataaa gtagctgtga agatactcaa tcgacagaag
     3181 attcggagcc ttgatgtggt aggaaaaatc cgcagagaaa ttcagaacct caagcttttc
     3241 aggcatcctc atataattaa actgtaccag gtcatcagta caccatctga tattttcatg
     3301 gtgatggaat atgtctcagg aggagagcta tttgattata tctgtaagaa tggaaggctg
     3361 gatgaaaaag aaagtcggcg tctgttccaa cagatccttt ctggtgtgga ttattgtcac
     3421 aggcatatgg tggtccatag agatttgaaa cctgaaaatg tcctgcttga tgcacacatg
     3481 aatgcaaaga tagctgattt tggtctttca aacatgatgt cagatggtga atttttaaga
     3541 acaagttgtg gctcacccaa ctatgctgca ccagaagtaa tttcaggaag attgtatgca
     3601 ggcccagagg tagatatatg gagcagtggg gttattctct atgctttatt atgtggaacc
     3661 cttccatttg atgatgacca tgtgccaact ctttttaaga agatatgtga tgggatcttc
     3721 tatacccctc aatatttaaa tccttctgtg attagccttt tgaaacatat gctgcaggtg
     3781 gatcccatga agagggccac aatcaaagat atcagggaac atgaatggtt taaacaggac
     3841 cttccaaaat atctctttcc tgaggatcca tcatatagtt caaccatgat tgatgatgaa
     3901 gccttaaaag aagtatgtga aaagtttgag tgctcagaag aggaagttct cagctgtctt
     3961 tacaacagaa atcaccagga tcctttggca gttgcctacc atctcataat agataacagg
     4021 agaataatga atgaagccaa agatttctat ttggcgacaa gcccacctga ttcttttctt
     4081 gatgatcatc acctgactcg gccccatcct gaaagagtac cattcttggt tgctgaaaca
     4141 ccaagggcac gccataccct tgatgaatta aatccacaga aatccaaaca ccaaggtgta
     4201 aggaaagcaa aatggcattt aggaattaga agtcaaagtc gaccaaatga tattatggca
     4261 gaagtatgta gagcaatcaa acaattggat tatgaatgga aggttgtaaa cccatattat
     4321 ttgcgtgtac gaaggaagaa tcctgtgaca agcacttact ccaaaatgag tctacagtta
     4381 taccaagtgg atagtagaac ttatctactg gatttccgta gtattgatga tgaaattaca
     4441 gaagccaaat cagggactgc tactccacag agatcgggat cagttagcaa ctatcgatct
     4501 tgccaaagga gtgattcaga tgctgaggct caaggaaaat cctcagaagt ttctcttacc
     4561 tcatctgtga cctcacttga ctcttctcct gttgacctaa ctccaagacc tggaagtcac
     4621 acaatagaat tttttgagat gtgtgcaaat ctaattaaaa ttcttgcaca ataaggatcc
     4681 gcggccgcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc
     4741 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg
     4801 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     4861 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     4921 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc
     4981 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc
     5041 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg
     5101 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
     5161 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
     5221 gtgaccggcg cctacgctag atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     5281 acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     5341 gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     5401 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     5461 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     5521 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     5581 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     5641 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     5701 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     5761 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     5821 cccgcaagcc cggtgcctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     5881 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5941 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6001 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6061 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6121 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6181 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6241 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6301 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6361 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6421 gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6481 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa
     6541 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6601 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6661 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6721 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6781 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     6841 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6901 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     6961 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7021 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     7081 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
     7141 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7201 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7261 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7321 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7381 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7441 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7501 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7561 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7621 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7681 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7741 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7801 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7861 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7921 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7981 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8041 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8101 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8161 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8221 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8281 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8341 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8401 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8461 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8521 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8581 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8641 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8701 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8761 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8821 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8881 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8941 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9001 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9061 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9121 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9181 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9241 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     9301 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     9361 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     9421 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     9481 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     9541 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     9601 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     9661 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     9721 gacgttgtaa aacgacggcc agtgccaagc tg
//
