LOCUS       V042399                10519 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042399
VERSION     V042399
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5373
                     /label="POP1(NM_015029)"
                     /note="POP1(NM_015029)"
                     /gene="POP1"
     CDS             5380..5445
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5489..5700
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5713..5981
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6008..6607
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6614..7202
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7276..7509
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7581..7702
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7721..7856
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7894..7910)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7918..7934
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7942..7972)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7987..8008
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8296..8884)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9055..9915)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9916..10020)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10494..10510
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtcaaat gcaaaagaaa gaaaacacgc caagaaaatg
     2341 agaaaccagc ctaccaatgt gactctgtcc tctggctttg tggctgacag aggtgtaaag
     2401 caccacagtg gaggtgaaaa acctttccaa gctcaaaaac aagagcctca tcctggaact
     2461 tcacgacagc ggcaaaccag agtcaacccc cattctctgc ctgaccctga agtgaatgag
     2521 cagtcttcct ccaaagggat gtttagaaaa aagggaggat ggaaagcagg tcccgagggc
     2581 acgtctcagg agatccccaa gtatataact gcttctactt ttgctcaagc acgagctgct
     2641 gaaatcagtg ctatgttaaa agctgtgacc cagaagtctt cgaattcact ggtttttcag
     2701 actctgccac ggcacatgcg acgaagagcc atgagccaca acgtcaaacg ccttcccaga
     2761 cggttacagg agattgccca gaaagaggcg gagaaagccg tacatcagaa aaaagaacat
     2821 tcaaaaaata aatgccataa agctcgaaga tgtcacatga accggacgct agaatttaac
     2881 cgtagacaaa agaagaacat ttggttagaa actcacatct ggcacgccaa gcggtttcat
     2941 atggtcaaga agtggggcta ctgccttggg gagaggccaa cagtcaagag ccacagagcc
     3001 tgctatcgag ccatgacgaa ccggtgcctc ctgcaggatt tatcctatta ctgttgtttg
     3061 gagttgaaag gcaaagagga agaaatacta aaggcgcttt ctggaatgtg taacatagac
     3121 acagggctga cgtttgcagc agttcactgc ttgtctggaa agcgccaagg gagccttgtg
     3181 ctttatcggg tgaataaata tcccagagaa atgcttgggc ctgttacgtt tatctggaag
     3241 tcccagagga ccccgggtga cccttctgag agcaggcagc tgtggatctg gctgcatcca
     3301 acccttaaac aggatatctt agaggaaata aaagcagcgt gccagtgtgt ggaacccatc
     3361 aaatcagctg tctgcatcgc tgacccactt ccaacaccat cccaagaaaa aagccaaact
     3421 gaattgcctg acgagaaaat tggcaagaaa agaaaaagga aagatgatgg agaaaatgct
     3481 aaaccaatta aaaaaattat cggtgatgga actagagatc catgtctacc atactcttgg
     3541 atctctccaa ccacaggcat tataatcagc gatttgacga tggagatgaa cagattccgg
     3601 ctgattgggc cactttccca ctccatccta actgaagcaa taaaagctgc ttctgtccac
     3661 actgtgggag aggacacaga ggagacacct caccgctggt ggatagaaac ctgtaagaaa
     3721 cctgacagcg tttcccttca ttgcagacaa gaagccattt tcgagttgtt gggaggaata
     3781 acatcaccag cagaaattcc ggcaggtact attctgggac tgacagttgg ggatcctcga
     3841 ataaatttgc cccaaaagaa gtccaaagct ttgcccaatc cagaaaaatg ccaagataat
     3901 gagaaagtta gacagctgct tctggagggt gtgcctgtgg aatgtacgca tagctttatc
     3961 tggaaccaag atatctgtaa gagtgtcaca gagaataaaa tctcggatca ggatttaaac
     4021 cggatgagga gtgaattgct ggtgcctggg tcacagctta ttttaggtcc ccatgaatcc
     4081 aagataccta tacttttgat tcagcagcca ggaaaagtga ctggtgaaga tcgactaggc
     4141 tggggaagtg gctgggatgt cctactccca aagggctggg gcatggcttt ctggattcca
     4201 tttatttatc gaggtgtgag agtcggaggg ttgaaagagt ctgcagtgca ttctcagtat
     4261 aagaggtcgc ctaatgtccc aggcgatttt ccagactgcc ctgccgggat gctgtttgcg
     4321 gaagagcaag ctaagaatct tcttgaaaag tacaaaagac gccctcctgc aaaacggccc
     4381 aactacgtta agcttggcac tctggcacct ttctgctgtc cctgggagca gttaactcaa
     4441 gactgggagt caagagtcca ggcttacgaa gaaccttctg tagcttcatc tccaaatggt
     4501 aaggagagtg acctaagaag atctgaggtg ccttgtgctc ccatgcctaa aaaaactcat
     4561 cagccatctg atgaagtggg cacatccata gagcacccca gggaggcaga ggaggtaatg
     4621 gatgcagggt gtcaagaatc ggcagggcct gagaggatca cagaccagga ggccagtgaa
     4681 aaccatgttg ctgccacagg gagtcacctc tgcgttctca ggagtagaaa attactgaag
     4741 caactgtcag cctggtgtgg gcccagttct gaggatagtc ggggaggccg gcgagctccc
     4801 ggcagaggcc agcaaggatt gaccagagag gcttgcctgt ccatcttggg ccacttcccc
     4861 agggccctgg tttgggtcag cctgtccctg ctcagcaagg gcagccccga gcctcacacc
     4921 atgatctgtg tcccagccaa ggaggacttc ctccagctcc atgaggactg gcattactgt
     4981 gggccccagg aatccaaaca cagtgaccca ttcaggagca agatcctgaa acagaaagag
     5041 aagaagaaaa gggagaagag gcagaagcca ggacgtgcct cttctgatgg cccggcgggg
     5101 gaagagcccg tggctgggca ggaagctctg actctagggc tgtggtcagg ccctctgccg
     5161 cgtgtgacgt tgcactgctc cagaactctc ctaggctttg tgactcaggg agatttttcc
     5221 atggctgttg gctgtggaga agccctgggg tttgttagct tgacaggctt gctggatatg
     5281 ctgtccagcc agcctgcagc gcagaggggc ttagtgctac tgaggcctcc cgcctctctg
     5341 cagtatcgat ttgcgaggat tgctattgag gtgggatccg actacaaaga ccatgacggt
     5401 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     5461 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5521 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5581 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5641 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5701 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5761 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5821 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5881 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5941 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     6001 acgctagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     6061 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6121 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6181 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6241 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6301 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6361 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6421 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6481 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6541 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6601 tgcctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6661 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6721 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6781 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6841 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6901 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6961 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     7021 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7081 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7141 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7201 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7261 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg
     7321 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7381 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7441 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7501 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7561 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     7621 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7681 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7741 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7801 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga
     7861 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7921 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7981 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8041 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8101 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8161 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8221 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8281 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8341 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8401 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8461 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8521 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8581 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8641 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8701 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8761 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8821 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8881 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8941 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9001 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9061 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9121 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9181 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9241 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9301 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9361 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9421 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9481 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9541 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9601 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9661 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9721 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9781 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9841 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9901 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9961 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10021 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10081 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    10141 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10201 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    10261 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    10321 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    10381 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    10441 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    10501 gacggccagt gccaagctg
//
