LOCUS       V044513                14983 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044513
VERSION     V044513
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..8226
                     /label="POLR2A(NM_000937)"
                     /note="POLR2A(NM_000937)"
                     /gene="POLR2A"
     CDS             8269..8334
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             8335..9081
                     /codon_start=1
                     /note="more active than the original APEX due to the A134P
                     mutation"
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell
                     proteomics (Lam et al., 2015)"
                     /transl_table=1
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
                     /label="APEX2"
     promoter        9125..9336
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             9349..9617
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      9646..9655
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             9676..10338
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             10408..10461
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             10462..11061
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    11062..11650
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11724..11957
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12029..12150
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      12190..12325
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(12358..12374)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12382..12398
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12406..12436)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12451..12472
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12760..13348)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13519..14379)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14380..14484)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14958..14974
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc acgggggtgg ccccccctcg
     2341 ggggacagcg catgcccgct gcgcaccatc aagagagtcc agttcggagt cctgagtccg
     2401 gatgaactga agcgaatgtc tgtgacggag ggtggcatca aatacccaga gacgactgag
     2461 ggaggccgcc ccaagcttgg ggggctgatg gacccgaggc agggggtgat tgagcggact
     2521 ggccgctgcc aaacatgtgc aggaaacatg acagagtgtc ctggccactt tggccacatt
     2581 gaactggcca agcctgtgtt tcacgtgggc ttcctggtga agacaatgaa agttttgcgc
     2641 tgtgtctgct tcttctgctc caaactgctt gtggactcta acaacccaaa gatcaaggat
     2701 atcctggcta agtccaaggg acagcccaag aagcggctca cacatgtcta cgacctttgc
     2761 aagggcaaaa acatatgcga gggtggggag gagatggaca acaagttcgg tgtggaacaa
     2821 cctgagggtg acgaggatct gaccaaagaa aagggccatg gtggctgtgg gcggtaccag
     2881 cccaggatcc ggcgttctgg cctagagctg tatgcggaat ggaagcacgt taatgaggac
     2941 tctcaggaga agaagatcct gctgagtcca gagcgagtgc atgagatctt caaacgcatc
     3001 tcagatgagg agtgttttgt gctgggcatg gagccccgct atgcacggcc agagtggatg
     3061 attgtcacag tgctgcctgt gcccccgctc tccgtgcggc ctgctgttgt gatgcagggc
     3121 tctgcccgta accaggatga cctgactcac aaactggctg acatcgtgaa gatcaacaat
     3181 cagctgcggc gcaatgagca gaacggcgca gcggcccatg tcattgcaga ggatgtgaag
     3241 ctcctccagt tccatgtggc caccatggtg gacaatgagc tgcctggctt gccccgtgcc
     3301 atgcagaagt ctgggcgtcc cctcaagtcc ctgaagcagc ggttgaaggg caaggaaggc
     3361 cgggtgcgag ggaacctgat gggcaaaaga gtggacttct cggcccgtac tgtcatcacc
     3421 cccgacccca acctctccat tgaccaggtt ggcgtgcccc gctccattgc tgccaacatg
     3481 acctttgcgg agattgtcac ccccttcaac attgacagac ttcaagaact agtgcgcagg
     3541 gggaacagcc agtacccagg cgccaagtac atcatccgag acaatggtga tcgcattgac
     3601 ttgcgtttcc accccaagcc cagtgacctt cacctgcaga ccggctataa ggtggaacgg
     3661 cacatgtgtg atggggacat tgttatcttc aaccggcagc caactctgca caaaatgtcc
     3721 atgatggggc atcgggtccg cattctccca tggtctacct ttcgcttgaa tcttagtgtg
     3781 acaactccgt acaatgcaga ctttgacggg gatgagatga acttgcacct gccacagtct
     3841 ctggagacgc gagcagagat ccaggagctg gccatggttc ctcgcatgat tgtcaccccc
     3901 cagagcaatc ggcctgtcat gggtattgtg caggacacac tcacagcagt gcgcaaattc
     3961 accaagagag acgtcttcct ggagcggggt gaagtgatga acctcctgat gttcctgtcg
     4021 acgtgggatg ggaaggtccc acagccggcc atcctaaagc cccggcccct gtggacaggc
     4081 aagcaaatct tctccctcat catacctggt cacatcaatt gtatccgtac ccacagcacc
     4141 catcccgatg atgaagacag tggcccttac aagcacatct ctcctgggga caccaaggtg
     4201 gtggtggaga atggggagct gatcatgggc atcctgtgta agaagtctct gggcacgtca
     4261 gctggctccc tggtccacat ctcctaccta gagatgggtc atgacatcac tcgcctcttc
     4321 tactccaaca ttcagactgt cattaacaac tggctcctca tcgagggtca tactattggc
     4381 attggggact ccattgctga ttctaagact taccaggaca ttcagaacac tattaagaag
     4441 gccaagcagg acgtaataga ggtcatcgag aaggcacaca acaatgagct ggagcccacc
     4501 ccagggaaca ctctgcggca gacgtttgag aatcaggtga accgcattct taacgatgcc
     4561 cgagacaaga ctggctcctc tgctcagaaa tccctgtctg aatacaataa cttcaagtct
     4621 atggtcgtgt ccggagctaa aggttccaag attaacatct cccaggtcat tgctgtcgtt
     4681 ggacagcaga acgtcgaggg caagcggatt ccatttggct tcaagcaccg gactctgcct
     4741 cacttcatca aggatgacta cgggcctgag agccgtggct ttgtggagaa ctcctaccta
     4801 gccggcctca cacccactga gttctttttc cacgccatgg ggggtcgtga ggggctcatt
     4861 gacacggctg tcaagactgc tgagactgga tacatccagc ggcggctgat caagtccatg
     4921 gagtcagtga tggtgaagta cgacgcgact gtgcggaact ccatcaacca ggtggtgcag
     4981 ctgcgctacg gcgaagacgg cctggcaggc gagagcgttg agttccagaa cctggctacg
     5041 cttaagcctt ccaacaaggc ttttgagaag aagttccgct ttgattatac caatgagagg
     5101 gccctgcggc gcactctgca ggaggacctg gtgaaggacg tgctgagcaa cgcacacatc
     5161 cagaacgagt tggagcggga atttgagcgg atgcgggagg atcgggaggt gctcagggtc
     5221 atcttcccaa ctggagacag caaggtcgtc ctcccctgta acctgctgcg gatgatctgg
     5281 aatgctcaga aaatcttcca catcaaccca cgccttccct ccgacctgca ccccatcaaa
     5341 gtggtggagg gagtcaagga attgagcaag aagctggtga ttgtgaatgg ggatgaccca
     5401 ctaagtcgac aggcccagga aaatgccacg ctgctcttca acatccacct gcggtccacg
     5461 ttgtgttccc gccgcatggc agaggagttt cggctcagtg gggaggcctt cgactggctg
     5521 cttggggaga ttgagtccaa gttcaaccaa gccattgcgc atcccgggga aatggtgggg
     5581 gctctggctg cgcagtccct tggagaacct gccacccaga tgaccttgaa taccttccac
     5641 tatgctggtg tgtctgccaa gaatgtgacg ctgggtgtgc cccgacttaa ggagctcatc
     5701 aacatttcca agaagccaaa gactccttcg cttactgtct tcctgttggg ccagtccgct
     5761 cgagatgctg agagagccaa ggatattctg tgccgtctgg agcatacaac gttgaggaag
     5821 gtgactgcca acacagccat ctactatgac cccaaccccc agagcacggt ggtggcagag
     5881 gatcaggaat gggtgaatgt ctactatgaa atgcctgact ttgatgtggc ccgaatctcc
     5941 ccctggctgt tgcgggtgga gctggatcgg aagcacatga ctgaccggaa gctcaccatg
     6001 gagcagattg ctgaaaagat caatgctggt tttggtgacg acttgaactg catctttaat
     6061 gatgacaatg cagagaagct ggtgctccgt attcgcatca tgaacagcga tgagaacaag
     6121 atgcaagagg aggaagaggt ggtggacaag atggatgatg atgtcttcct gcgctgcatc
     6181 gagtccaaca tgctgacaga tatgaccctg cagggcatcg agcagatcag caaggtgtac
     6241 atgcacttgc cacagacaga caacaagaag aagatcatca tcacggagga tggggaattc
     6301 aaggccctgc aggagtggat cctggagacg gacggcgtga gcttgatgcg ggtgctgagt
     6361 gagaaggacg tggaccccgt acgcaccacg tccaatgaca ttgtggagat cttcacggtg
     6421 ctgggcattg aagccgtgcg gaaggccctg gagcgggagc tgtaccacgt catctccttt
     6481 gatggctcct atgtcaatta ccgacacttg gctctcttgt gtgataccat gacctgtcgt
     6541 ggccacttga tggccatcac ccgacacgga gtcaaccgcc aggacacagg accactcatg
     6601 aagtgttcct ttgaggaaac ggtggacgtg cttatggaag cagccgcaca cggtgagagt
     6661 gaccccatga agggggtctc tgagaatatc atgctgggcc agctggctcc ggccggcact
     6721 ggctgctttg acctcctgct tgatgcagag aagtgcaagt atggcatgga gatccccacc
     6781 aatatccccg gcctgggggc tgctggaccc accggcatgt tctttggttc agcacccagt
     6841 cccatgggtg gaatctctcc tgccatgaca ccttggaacc agggtgcaac ccctgcctat
     6901 ggcgcctggt cccccagtgt tgggagtgga atgaccccag gggcagccgg cttctctccc
     6961 agtgctgcgt cagatgccag cggcttcagc ccaggttact cccctgcctg gtctcccaca
     7021 ccgggctccc cggggtcccc aggtccctca agcccctaca tcccttcacc aggtggtgcc
     7081 atgtctccca gctactcgcc aacgtcacct gcctacgagc cccgctctcc tgggggctac
     7141 acaccccaga gtccctctta ttcccccact tcaccctcct actcccctac ctctccatcc
     7201 tattctccaa ccagtcccaa ctatagtccc acatcaccca gctattcgcc aacgtcaccc
     7261 agctactcac cgacctctcc cagctactca cccacctctc ccagctactc gcccacctct
     7321 cccagctact cgcccacctc tcccagctac tcacccactt cccctagcta ctcgcccact
     7381 tcccctagct actcgccaac gtctcccagc tactcgccga catctcccag ctactcgcca
     7441 acttcaccca gctattctcc cacttctccc agctactcac ctacctctcc aagctattca
     7501 cccacctccc ccagctactc acccacttcc ccaagttact cacccaccag cccgaactat
     7561 tctccaacca gtcccaatta caccccaaca tcacccagct acagcccgac atcacccagc
     7621 tattcaccta ctagtcccaa ctacacacct accagcccta actacagccc aacctctcca
     7681 agctactctc caacatcacc cagctattcc ccgacctcac caagttactc cccttccagc
     7741 ccacgataca caccacagtc tccaacctat accccaagct cacccagcta cagccccagc
     7801 tcgcccagct acagcccaac ctcacccaag tacaccccaa ccagtccttc ttacagtccc
     7861 agctccccag agtatacccc aacctctccc aagtactcac ctaccagtcc caaatattca
     7921 cccacctctc ccaagtactc gcctaccagt cccacctatt cacccaccac cccaaaatac
     7981 tccccaacat ctcctactta ttccccaacc tctccagtct acaccccaac ctctcccaag
     8041 tactcaccta ctagccccac ttactcgccc acttccccca agtactcgcc caccagcccc
     8101 acctactcgc ccacctcccc caaaggctca acctactctc ccacttcccc tggttactcg
     8161 cccaccagcc ccacctacag tctcacaagc ccggctatca gcccggatga cagtgacgag
     8221 gagaacggat ccggtggagg cggatcagga ggcggtggat ctggatccga ctacaaagac
     8281 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagggaaag
     8341 tcttacccaa ctgtgagtgc tgattaccag gacgccgttg agaaggcgaa gaagaagctc
     8401 agaggcttca tcgctgagaa gagatgcgct cctctaatgc tccgtttggc attccactct
     8461 gctggaacct ttgacaaggg cacgaagacc ggtggaccct tcggaaccat caagcaccct
     8521 gccgaactgg ctcacagcgc taacaacggt cttgacatcg ctgttaggct tttggagcca
     8581 ctcaaggcgg agttccctat tttgagctac gccgatttct accagttggc tggcgttgtt
     8641 gccgttgagg tcacgggtgg acctaaggtt ccattccacc ctggaagaga ggacaagcct
     8701 gagccaccac cagagggtcg cttgcctgat cccactaagg gttctgacca tttgagagat
     8761 gtgtttggca aagctatggg gcttactgac caagatatcg ttgctctatc tgggggtcac
     8821 actattggag ctgcacacaa ggagcgttct ggatttgagg gtccctggac ctctaatcct
     8881 cttattttcg acaactcata cttcacggag ttgttgagtg gtgagaagga aggtctcctt
     8941 cagctacctt ctgacaaggc tcttttgtct gaccctgtat tccgccctct cgttgataaa
     9001 tatgcagcgg acgaagatgc cttctttgct gattacgctg aggctcacca aaagctttcc
     9061 gagcttgggt ttgctgatgc ttagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     9121 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     9181 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     9241 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     9301 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     9361 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     9421 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     9481 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     9541 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     9601 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     9661 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     9721 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     9781 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     9841 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     9901 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     9961 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
    10021 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
    10081 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
    10141 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
    10201 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
    10261 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
    10321 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
    10381 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
    10441 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
    10501 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
    10561 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
    10621 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
    10681 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
    10741 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
    10801 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
    10861 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
    10921 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
    10981 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
    11041 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
    11101 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
    11161 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
    11221 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
    11281 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
    11341 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
    11401 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
    11461 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
    11521 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
    11581 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
    11641 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
    11701 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
    11761 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    11821 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    11881 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    11941 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
    12001 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
    12061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    12121 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
    12181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    12241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    12301 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
    12361 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    12421 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    12481 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    12541 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    12601 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    12661 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    12721 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    12781 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    12841 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    12901 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    12961 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    13021 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    13081 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    13141 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    13201 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    13261 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    13321 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    13381 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    13441 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    13501 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    13561 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    13621 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    13681 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    13741 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    13801 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    13861 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    13921 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    13981 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    14041 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    14101 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    14161 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    14221 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    14281 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    14341 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    14401 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    14461 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    14521 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    14581 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    14641 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    14701 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    14761 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    14821 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    14881 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    14941 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
