LOCUS       V033516                10720 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033516
VERSION     V033516
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4746
                     /label="PLG(NM_000301)"
                     /note="PLG(NM_000301)"
                     /gene="PLG"
     CDS             4753..4818
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4862..5073
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5086..5354
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5383..5392
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5413..6075
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6145..6198
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6199..6798
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6799..7387
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7461..7694
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7766..7887
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7927..8062
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8095..8111)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8119..8135
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8143..8173)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8188..8209
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8497..9085)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9256..10116)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10117..10221)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10695..10711
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aacataagga agtggttctt
     2341 ctacttcttt tatttctgaa atcaggtcaa ggagagcctc tggatgacta tgtgaatacc
     2401 cagggggctt cactgttcag tgtcactaag aagcagctgg gagcaggaag tatagaagaa
     2461 tgtgcagcaa aatgtgagga ggacgaagaa ttcacctgca gggcattcca atatcacagt
     2521 aaagagcaac aatgtgtgat aatggctgaa aacaggaagt cctccataat cattaggatg
     2581 agagatgtag ttttatttga aaagaaagtg tatctctcag agtgcaagac tgggaatgga
     2641 aagaactaca gagggacgat gtccaaaaca aaaaatggca tcacctgtca aaaatggagt
     2701 tccacttctc cccacagacc tagattctca cctgctacac acccctcaga gggactggag
     2761 gagaactact gcaggaatcc agacaacgat ccgcaggggc cctggtgcta tactactgat
     2821 ccagaaaaga gatatgacta ctgcgacatt cttgagtgtg aagaggaatg tatgcattgc
     2881 agtggagaaa actatgacgg caaaatttcc aagaccatgt ctggactgga atgccaggcc
     2941 tgggactctc agagcccaca cgctcatgga tacattcctt ccaaatttcc aaacaagaac
     3001 ctgaagaaga attactgtcg taaccccgat agggagctgc ggccttggtg tttcaccacc
     3061 gaccccaaca agcgctggga actttgtgac atcccccgct gcacaacacc tccaccatct
     3121 tctggtccca cctaccagtg tctgaaggga acaggtgaaa actatcgcgg gaatgtggct
     3181 gttaccgtgt ccgggcacac ctgtcagcac tggagtgcac agacccctca cacacataac
     3241 aggacaccag aaaacttccc ctgcaaaaat ttggatgaaa actactgccg caatcctgac
     3301 ggaaaaaggg ccccatggtg ccatacaacc aacagccaag tgcggtggga gtactgtaag
     3361 ataccgtcct gtgactcctc cccagtatcc acggaacaat tggctcccac agcaccacct
     3421 gagctaaccc ctgtggtcca ggactgctac catggtgatg gacagagcta ccgaggcaca
     3481 tcctccacca ccaccacagg aaagaagtgt cagtcttggt catctatgac accacaccgg
     3541 caccagaaga ccccagaaaa ctacccaaat gctggcctga caatgaacta ctgcaggaat
     3601 ccagatgccg ataaaggccc ctggtgtttt accacagacc ccagcgtcag gtgggagtac
     3661 tgcaacctga aaaaatgctc aggaacagaa gcgagtgttg tagcacctcc gcctgttgtc
     3721 ctgcttccag atgtagagac tccttccgaa gaagactgta tgtttgggaa tgggaaagga
     3781 taccgaggca agagggcgac cactgttact gggacgccat gccaggactg ggctgcccag
     3841 gagccccata gacacagcat tttcactcca gagacaaatc cacgggcggg tctggaaaaa
     3901 aattactgcc gtaaccctga tggtgatgta ggtggtccct ggtgctacac gacaaatcca
     3961 agaaaacttt acgactactg tgatgtccct cagtgtgcgg ccccttcatt tgattgtggg
     4021 aagcctcaag tggagccgaa gaaatgtcct ggaagggttg taggggggtg tgtggcccac
     4081 ccacattcct ggccctggca agtcagtctt agaacaaggt ttggaatgca cttctgtgga
     4141 ggcaccttga tatccccaga gtgggtgttg actgctgccc actgcttgga gaagtcccca
     4201 aggccttcat cctacaaggt catcctgggt gcacaccaag aagtgaatct cgaaccgcat
     4261 gttcaggaaa tagaagtgtc taggctgttc ttggagccca cacgaaaaga tattgccttg
     4321 ctaaagctaa gcagtcctgc cgtcatcact gacaaagtaa tcccagcttg tctgccatcc
     4381 ccaaattatg tggtcgctga ccggaccgaa tgtttcatca ctggctgggg agaaacccaa
     4441 ggtacttttg gagctggcct tctcaaggaa gcccagctcc ctgtgattga gaataaagtg
     4501 tgcaatcgct atgagtttct gaatggaaga gtccaatcca ccgaactctg tgctgggcat
     4561 ttggccggag gcactgacag ttgccagggt gacagtggag gtcctctggt ttgcttcgag
     4621 aaggacaaat acattttaca aggagtcact tcttggggtc ttggctgtgc acgccccaat
     4681 aagcctggtg tctatgttcg tgtttcaagg tttgttactt ggattgaggg agtgatgaga
     4741 aataatggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     4801 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     4861 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     4921 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     4981 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5041 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5101 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5161 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5221 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     5281 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     5341 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     5401 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     5461 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     5521 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     5581 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     5641 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     5701 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     5761 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     5821 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     5881 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     5941 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     6001 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     6061 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     6121 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     6181 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6241 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6301 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6361 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6421 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6481 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6541 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6601 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6661 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6721 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6781 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6841 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6901 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6961 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7021 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7081 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7141 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7201 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7261 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7321 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7381 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7441 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     7501 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7561 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7621 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7681 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7741 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     7801 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7861 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7921 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7981 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8041 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     8101 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8161 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8221 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8281 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8341 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8401 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8461 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8521 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8581 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8641 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8701 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8761 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8821 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8881 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8941 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9001 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9061 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9121 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9361 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9781 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9961 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10021 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10081 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10141 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10201 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10261 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10321 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10381 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10441 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    10501 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    10561 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10621 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10681 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
