LOCUS       V032257                11839 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032257
VERSION     V032257
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5857
                     /label="PLCB2(NM_004573)"
                     /note="PLCB2(NM_004573)"
                     /gene="PLCB2"
     CDS             5864..5953
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5998..6209
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6222..6490
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6519..6528
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6549..7211
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7281..7334
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7335..7934
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7935..8522
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8596..8829
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8901..9022
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9041..9176
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9214..9230)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9238..9254
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9262..9292)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9307..9328
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9616..10204)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10375..11235)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11236..11340)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11814..11830
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgtctct gctcaaccct gtcctgctgc cccccaaggt
     2341 gaaggcctat ctgagccaag gggagcgctt catcaaatgg gatgatgaaa ctacagttgc
     2401 ctctccagtt atcctccgtg tggatcctaa gggctactac ttatactgga cgtatcaaag
     2461 taaggagatg gagtttctgg atatcaccag catccgggat actcgctttg ggaagtttgc
     2521 caagatgccc aagagccaga agctccggga cgtcttcaac atggactttc ctgataacag
     2581 tttcctgctg aagacactca cggtggtgtc cggcccggac atggtggacc tcaccttcca
     2641 caacttcgtc tcctacaagg agaacgtggg caaggcctgg gctgaggacg tactggccct
     2701 agtcaaacat ccgctgacgg ccaacgcctc ccgcagcacc ttcctggaca agatccttgt
     2761 gaagctcaag atgcagctca actctgaagg gaagattccg gtgaagaact ttttccagat
     2821 gtttcctgct gaccgcaagc gggtggaagc tgctctcagt gcctgccacc tccccaaagg
     2881 caaaaatgac gccatcaatc ctgaggactt cccagaacct gtctacaaga gtttcctcat
     2941 gagcctctgt cctcggccag aaatagatga gatcttcact tcttaccatg ctaaggccaa
     3001 accctacatg acgaaggagc acctgaccaa attcatcaac cagaaacagc gggactcccg
     3061 gcttaactcc ctgctgttcc cgccagcacg gcctgaccag gtgcagggcc tcatcgacaa
     3121 gtatgagccc agtggcatca atgcacagag gggccagctg tcacctgaag gcatggtctg
     3181 gtttctctgt gggccagaga acagcgtgct ggcccaggac aagctgctgc tccaccacga
     3241 catgacgcag ccactcaatc attacttcat caactcgtcc cacaacacct acctgacagc
     3301 cggccagttc tcaggcctct cctcggctga gatgtaccgc caggtgctgc tctctggctg
     3361 ccgttgcgtg gagctagact gctggaaggg gaaaccccct gacgaggagc ccattatcac
     3421 ccatggcttc accatgacca cagacatctt cttcaaagaa gcaattgagg ctattgcaga
     3481 aagcgccttt aagacctccc cctatcccat catcctgtcg tttgagaacc atgtggactc
     3541 accccgccag caggctaaga tggctgagta ttgccggacg atctttgggg atatgctgct
     3601 cacagagccc ctggaaaagt tcccactaaa accaggtgtc cccctgccca gccctgagga
     3661 tctcaggggc aagatcctca tcaagaacaa gaagaaccag ttttctggcc ccacctcctc
     3721 cagtaaggat actggtgggg aggctgaggg cagcagccca cccagtgccc ctgcaggtga
     3781 gggcacagtg tgggctggcg aggaagggac tgagctggag gaggaggagg tggaagagga
     3841 agaggaggag gagtcaggaa acctggatga agaagagatt aagaagatgc agtcggatga
     3901 gggcacagcg ggcctggaag tgacggctta tgaggagatg tccagcctag tcaattacat
     3961 ccagcccacc aagttcgtct cctttgagtt ctctgcccaa aagaaccgaa gttatgtcat
     4021 ctcgtccttc acagagctca aggcatatga cctgctctcc aaggcctcgg tgcagtttgt
     4081 ggactacaac aagcgccaga tgagccgcat ttaccccaag ggaacccgca tggactcctc
     4141 caactacatg ccccagatgt tctggaatgc tggatgccag atggttgccc tcaacttcca
     4201 gacgatggac ttgcccatgc agcagaacat ggcagtattt gagttcaacg ggcagagcgg
     4261 ctacctcctc aagcatgagt tcatgcgccg gccggacaag cagttcaacc ccttctcagt
     4321 ggaccgcatc gacgtggtgg tggccaccac cctttccatt acggtgatct ctgggcagtt
     4381 cctgtcagaa cgcagcgtgc gcacctatgt agaagtggag ctgtttggcc ttcctgggga
     4441 ccccaagagg cgctatcgaa ctaagctgtc acccagtact aactccatca atcctgtctg
     4501 gaaggaggag ccctttgtct ttgagaagat cttgatgcct gagctggcct ccctcagagt
     4561 ggctgtgatg gaggaaggca acaagtttct tggacaccgc atcatcccca tcaatgccct
     4621 aaattctggg taccaccacc tgtgcctgca cagtgagagc aacatgcccc tcaccatgcc
     4681 tgcgctcttc atcttcctgg agatgaagga ctacatacct ggtgcttggg cagatctcac
     4741 tgtggccctc gccaacccca ttaagttctt cagtgcccat gacacgaagt ctgtgaagct
     4801 caaggaggcc atgggaggtc tgcctgagaa gcccttccca ctggcgagtc cagttgccag
     4861 ccaggtcaat ggggcgttgg ccccaacgag caatgggtca ccagcagcca gggccggggc
     4921 cagggaagag gctatgaaag aagctgcgga gccgcggacc gccagcctgg aggagctccg
     4981 ggagctaaag ggcgtggtga agctgcagcg gcggcacgag aaggagctgc gagagttgga
     5041 gcggcgcgga gcgcggcgct gggaggagct gctgcagcgg ggcgcggcgc agctggcgga
     5101 gctcgggcca ccgggcgtgg ggggcgtcgg ggcctgcaag ctcggtcccg gcaagggctc
     5161 tcgcaagaag aggagcctgc cccgcgagga gagcgccgga gccgcgccgg gcgagggccc
     5221 tgagggcgtg gacgggcgcg tgcgggagct gaaagacagg ctggagctgg agctgctgcg
     5281 gcagggcgag gagcagtacg agtgcgttct gaagcgcaag gagcagcacg tggccgagca
     5341 aatctccaaa atgatggagc tggccagaga gaaacaggcg gcagagctga aggccctgaa
     5401 ggagacgtcg gagaacgaca ccaaagagat gaagaaaaag ctggagacaa agagactgga
     5461 gcggatccag ggcatgacca aagtcaccac agacaagatg gcccaggaga ggttgaagag
     5521 agagattaac aactcccaca tccaggaagt agtgcaggtg atcaagcaga tgacggagaa
     5581 cttggagagg caccaggaga agctggagga gaagcaggcg gcttgcctgg aacagatacg
     5641 ggagatggaa aagcagttcc agaaggaggc gctggcagag tacgaggcca ggatgaaggg
     5701 tctggaggca gaggtgaagg agtcggtgag ggcctgcctc aggacctgct ttccctccga
     5761 ggccaaggac aagcctgaga gggcctgcga gtgcccccca gagctgtgtg agcaggaccc
     5821 actcatagca aaggcagatg cccaggagag ccgcctcctc gaggaacaaa aactaatatc
     5881 agaggaagat ttggaacaga aactaatcag tgaagaagat ttagagcaaa agttaatttc
     5941 cgaggaggac ttataggcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg
     6001 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg
     6061 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     6121 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     6181 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt
     6241 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc
     6301 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt
     6361 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca
     6421 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc
     6481 gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag agcgacgaga
     6541 gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt
     6601 tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga
     6661 agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg
     6721 gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca
     6781 acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg
     6841 tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca
     6901 ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg
     6961 tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca
     7021 gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg
     7081 ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg
     7141 agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca
     7201 tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac
     7261 ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg
     7321 agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     7381 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     7441 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     7501 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     7561 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     7621 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7681 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7741 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     7801 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7861 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7921 agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7981 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     8041 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     8101 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     8161 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     8221 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     8281 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     8341 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     8401 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     8461 ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc
     8521 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8581 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg
     8641 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8701 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8761 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8821 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     8881 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     8941 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9001 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     9061 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9121 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga
     9181 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     9241 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     9301 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     9361 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9421 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9481 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9541 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    10021 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    10081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    10141 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    10201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10261 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    10321 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    10381 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10441 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10501 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10561 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10621 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10681 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10741 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10801 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10861 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10921 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10981 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    11041 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    11101 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    11161 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    11221 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    11281 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    11341 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    11401 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    11461 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    11521 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    11581 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    11641 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    11701 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    11761 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    11821 gacggccagt gccaagctg
//
