LOCUS       V021849                10357 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021849
VERSION     V021849
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2301..3008
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3042..5282
                     /label="PKP1(NM_000299)"
                     /note="PKP1(NM_000299)"
                     /gene="PKP1"
     promoter        5327..5538
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5551..5819
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5846..6445
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6452..7040
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7114..7347
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7419..7540
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7559..7694
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7732..7748)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7756..7772
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7780..7810)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7825..7846
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8134..8722)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8893..9753)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9754..9858)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10332..10348
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcgccacc atggtgagca agggcgagga ggataacatg gccatcatca
     2341 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     2401 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     2461 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     2521 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     2581 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     2641 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     2701 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     2761 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     2821 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     2881 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     2941 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     3001 tgtacaagtc cggactcaga tctcgaggag gagggggaag catgaaccac tcgccgctca
     3061 agaccgcctt ggcgtacgaa tgcttccagg accaggacaa ctccacgttg gctttgccgt
     3121 cggaccaaaa gatgaaaaca ggcacgtctg gcaggcagcg cgtgcaggag caggtgatga
     3181 tgaccgtcaa gcggcagaag tccaagtctt cccagtcgtc caccctgagc cactccaatc
     3241 gaggttccat gtatgatggc ttggctgaca attacaacta tgggaccacc agcaggagca
     3301 gctactactc caagttccag gcagggaatg gctcatgggg atatccgatc tacaatggaa
     3361 ccctcaagcg ggagcctgac aacaggcgct tcagctccta cagccagatg gagaactgga
     3421 gccggcacta cccccggggc agctgtaaca ccaccggcgc aggcagcgac atctgcttca
     3481 tgcagaaaat caaggcgagc cgcagtgagc ccgacctcta ctgtgaccca cggggcaccc
     3541 tgcgcaaggg cacgctgggc agcaagggcc agaagaccac ccagaaccgc tacagctttt
     3601 acagcacctg cagtggtcag aaggccataa agaagtgccc tgtgcgcccg ccctcttgtg
     3661 cctccaagca ggaccctgtg tatatcccgc ccatctcctg caacaaggac ctgtcctttg
     3721 gccactctag ggccagctcc aagatctgca gtgaggacat cgagtgcagt gggctgacca
     3781 tccccaaggc tgtgcagtac ctgagctccc aggatgagaa gtaccaggcc attggggcct
     3841 attacatcca gcatacctgc ttccaggatg aatctgccaa gcaacaggtc tatcagctgg
     3901 gaggcatctg caagctggtg gacctcctcc gcagccccaa ccagaacgtc cagcaggccg
     3961 cggcaggggc cctgcgcaac ctggtgttca ggagcaccac caacaagctg gagacccgga
     4021 ggcagaatgg gatccgcgag gcagtcagcc tcctgaggag aaccgggaac gccgagatcc
     4081 agaagcagct gactgggctg ctctggaacc tgtcttccac tgacgagctg aaggaggaac
     4141 tcattgccga cgccctgcct gttctggccg accgcgtcat cattcccttc tctggctggt
     4201 gcgatggcaa tagcaacatg tcccgggaag tggtggaccc tgaggtcttc ttcaatgcca
     4261 caggctgctt gagaaagaga ctgggcatgc gggagcttct ggctcttgtt ccgcaaaggg
     4321 ccactagtag cagggtgaac ctgagctcgg ccgatgcagg ccgccagacc atgcgtaact
     4381 actcagggct cattgattcc ctcatggcct atgtccagaa ctgtgtagcg gccagccgct
     4441 gtgacgacaa gtctgtggaa aactgcatgt gtgttctgca caacctctcc taccgcctgg
     4501 acgccgaggt gcccacccgc taccgccagc tggagtataa cgcccgcaac gcctacaccg
     4561 agaagtcctc cactggctgc ttcagcaaca agagcgacaa gatgatgaac aacaactatg
     4621 actgccccct gcctgaggaa gagaccaacc ccaagggcag cggctggttg taccattcag
     4681 atgccatccg cacctacctg aacctcatgg gcaagagcaa gaaagatgct accctggagg
     4741 cctgtgctgg tgccctgcag aacctgacag ccagcaaggg gctgatgtcc agtggcatga
     4801 gccagttgat tgggctgaag gaaaagggcc tgccacaaat tgcccgcctc ctgcaatctg
     4861 gcaactctga tgtggtgcgg tccggagcct ccctcctgag caacatgtcc cgccaccctc
     4921 tgctgcacag agtgatgggg aaccaggtgt tcccggaggt gaccaggctc ctcaccagcc
     4981 acactggcaa taccagcaac tccgaagaca tcttgtcctc ggcctgctac actgtgagga
     5041 acctgatggc ctcgcagcca caactggcca agcagtactt ctccagcagc atgctcaaca
     5101 acatcatcaa cctgtgccga agcagtgcct cacccaaggc cgcagaagct gcccggcttc
     5161 tcctgtctga catgtggtcc agcaaggaac tgcagggtgt cctcagacag caaggtttcg
     5221 ataggaacat gctgggaacc ttagctgggg ccaacagcct caggaacttc acctcccgat
     5281 tctaagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     5341 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga
     5401 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     5461 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     5521 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     5581 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     5641 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     5701 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     5761 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     5821 aagctgtgac cggcgcctac gctagatgac cgagtacaag cccacggtgc gcctcgccac
     5881 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     5941 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     6001 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     6061 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     6121 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     6181 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     6241 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     6301 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     6361 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     6421 catgacccgc aagcccggtg cctgagtcga caatcaacct ctggattaca aaatttgtga
     6481 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6541 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6601 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6661 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6721 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6781 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6841 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6901 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6961 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7021 cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag
     7081 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7141 aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7201 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7261 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7321 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     7381 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7441 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7501 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7561 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7621 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7681 cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct
     7741 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7801 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7861 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7921 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7981 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8041 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8101 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8161 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8221 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8281 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8341 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8401 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8461 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8521 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     8581 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8641 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8701 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8761 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8821 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8881 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8941 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9001 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9061 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9121 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9181 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9241 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9301 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9361 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9421 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9481 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9541 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9601 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9661 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9721 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9781 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9841 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     9901 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     9961 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    10021 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    10081 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    10141 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    10201 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    10261 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    10321 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
