LOCUS       V043321                11419 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043321
VERSION     V043321
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2279..2288
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2288..2311
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             2324..5527
                     /label="PIK3CA(NM_006218)"
                     /note="PIK3CA(NM_006218)"
                     /gene="PIK3CA"
     promoter        5578..5789
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5802..6070
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6099..6108
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6129..6791
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6861..6914
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6915..7514
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7515..8102
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8176..8409
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8481..8602
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8621..8756
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8794..8810)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8818..8834
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8842..8872)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8887..8908
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9196..9784)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9955..10815)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10816..10920)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11394..11410
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatggat tacaaggatg acgacgataa ggctagcgaa ttcatgcctc cacgaccatc
     2341 atcaggtgaa ctgtggggca tccacttgat gcccccaaga atcctagtag aatgtttact
     2401 accaaatgga atgatagtga ctttagaatg cctccgtgag gctacattaa taaccataaa
     2461 gcatgaacta tttaaagaag caagaaaata ccccctccat caacttcttc aagatgaatc
     2521 ttcttacatt ttcgtaagtg ttactcaaga agcagaaagg gaagaatttt ttgatgaaac
     2581 aagacgactt tgtgaccttc ggctttttca acccttttta aaagtaattg aaccagtagg
     2641 caaccgtgaa gaaaagatcc tcaatcgaga aattggtttt gctatcggca tgccagtgtg
     2701 tgaatttgat atggttaaag atccagaagt acaggacttc cgaagaaata ttctgaacgt
     2761 ttgtaaagaa gctgtggatc ttagggacct caattcacct catagtagag caatgtatgt
     2821 ctatcctcca aatgtagaat cttcaccaga attgccaaag cacatatata ataaattaga
     2881 taaagggcaa ataatagtgg tgatctgggt aatagtttct ccaaataatg acaagcagaa
     2941 gtatactctg aaaatcaacc atgactgtgt accagaacaa gtaattgctg aagcaatcag
     3001 gaaaaaaact cgaagtatgt tgctatcctc tgaacaacta aaactctgtg ttttagaata
     3061 tcagggcaag tatattttaa aagtgtgtgg atgtgatgaa tacttcctag aaaaatatcc
     3121 tctgagtcag tataagtata taagaagctg tataatgctt gggaggatgc ccaatttgat
     3181 gttgatggct aaagaaagcc tttattctca actgccaatg gactgtttta caatgccatc
     3241 ttattccaga cgcatttcca cagctacacc atatatgaat ggagaaacat ctacaaaatc
     3301 cctttgggtt ataaatagtg cactcagaat aaaaattctt tgtgcaacct acgtgaatgt
     3361 aaatattcga gacattgata agatctatgt tcgaacaggt atctaccatg gaggagaacc
     3421 cttatgtgac aatgtgaaca ctcaaagagt accttgttcc aatcccaggt ggaatgaatg
     3481 gctgaattat gatatataca ttcctgatct tcctcgtgct gctcgacttt gcctttccat
     3541 ttgctctgtt aaaggccgaa agggtgctaa agaggaacac tgtccattgg catggggaaa
     3601 tataaacttg tttgattaca cagacactct agtatctgga aaaatggctt tgaatctttg
     3661 gccagtacct catggattag aagatttgct gaaccctatt ggtgttactg gatcaaatcc
     3721 aaataaagaa actccatgct tagagttgga gtttgactgg ttcagcagtg tggtaaagtt
     3781 cccagatatg tcagtgattg aagagcatgc caattggtct gtatcccgag aagcaggatt
     3841 tagctattcc cacgcaggac tgagtaacag actagctaga gacaatgaat taagggaaaa
     3901 tgacaaagaa cagctcaaag caatttctac acgagatcct ctctctgaaa tcactgagca
     3961 ggagaaagat tttctatgga gtcacagaca ctattgtgta actatccccg aaattctacc
     4021 caaattgctt ctgtctgtta aatggaattc tagagatgaa gtagcccaga tgtattgctt
     4081 ggtaaaagat tggcctccaa tcaaacctga acaggctatg gaacttctgg actgtaatta
     4141 cccagatcct atggttcgag gttttgctgt tcggtgcttg gaaaaatatt taacagatga
     4201 caaactttct cagtatttaa ttcagctagt acaggtccta aaatatgaac aatatttgga
     4261 taacttgctt gtgagatttt tactgaagaa agcattgact aatcaaagga ttgggcactt
     4321 tttcttttgg catttaaaat ctgagatgca caataaaaca gttagccaga ggtttggcct
     4381 gcttttggag tcctattgtc gtgcatgtgg gatgtatttg aagcacctga ataggcaagt
     4441 cgaggcaatg gaaaagctca ttaacttaac tgacattctc aaacaggaga agaaggatga
     4501 aacacaaaag gtacagatga agtttttagt tgagcaaatg aggcgaccag atttcatgga
     4561 tgctctacag ggctttctgt ctcctctaaa ccctgctcat caactaggaa acctcaggct
     4621 tgaagagtgt cgaattatgt cctctgcaaa aaggccactg tggttgaatt gggagaaccc
     4681 agacatcatg tcagagttac tgtttcagaa caatgagatc atctttaaaa atggggatga
     4741 tttacggcaa gatatgctaa cacttcaaat tattcgtatt atggaaaata tctggcaaaa
     4801 tcaaggtctt gatcttcgaa tgttacctta tggttgtctg tcaatcggtg actgtgtggg
     4861 acttattgag gtggtgcgaa attctcacac tattatgcaa attcagtgca aaggcggctt
     4921 gaaaggtgca ctgcagttca acagccacac actacatcag tggctcaaag acaagaacaa
     4981 aggagaaata tatgatgcag ccattgacct gtttacacgt tcatgtgctg gatactgtgt
     5041 agctaccttc attttgggaa ttggagatcg tcacaatagt aacatcatgg tgaaagacga
     5101 tggacaactg tttcatatag attttggaca ctttttggat cacaagaaga aaaaatttgg
     5161 ttataaacga gaacgtgtgc catttgtttt gacacaggat ttcttaatag tgattagtaa
     5221 aggagcccaa gaatgcacaa agacaagaga atttgagagg tttcaggaga tgtgttacaa
     5281 ggcttatcta gctattcgac agcatgccaa tctcttcata aatcttttct caatgatgct
     5341 tggctctgga atgccagaac tacaatcttt tgatgacatt gcatacattc gaaagaccct
     5401 agccttagat aaaactgagc aagaggcttt ggagtatttc atgaaacaaa tgaatgatgc
     5461 acatcatggt ggctggacaa caaaaatgga ttggatcttc cacacaatta aacagcatgc
     5521 attgaactga ggatccgcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg
     5581 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg
     5641 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     5701 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     5761 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt
     5821 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc
     5881 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt
     5941 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca
     6001 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc
     6061 gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag agcgacgaga
     6121 gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt
     6181 tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga
     6241 agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg
     6301 gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca
     6361 acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg
     6421 tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca
     6481 ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg
     6541 tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca
     6601 gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg
     6661 ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg
     6721 agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca
     6781 tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac
     6841 ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg
     6901 agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     6961 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     7021 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     7081 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     7141 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     7201 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7261 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7321 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     7381 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7441 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7501 agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7561 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7621 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7681 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7741 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7801 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7861 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     7921 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7981 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     8041 ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc
     8101 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8161 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg
     8221 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8281 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8341 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8401 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     8461 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     8521 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8581 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     8641 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8701 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga
     8761 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8821 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8881 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8941 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9001 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9061 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9601 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10921 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10981 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    11041 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    11101 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    11161 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    11221 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    11281 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    11341 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    11401 gacggccagt gccaagctg
//
