LOCUS       V028129                15853 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028129
VERSION     V028129
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..9879
                     /label="PIEZO1(NM_001142864)"
                     /note="PIEZO1(NM_001142864)"
                     /gene="PIEZO1"
     CDS             9886..9951
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        9995..10206
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             10219..10487
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      10516..10525
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             10546..11208
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             11278..11331
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             11332..11931
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    11932..12520
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             12594..12827
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12899..13020
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      13060..13195
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(13228..13244)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    13252..13268
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(13276..13306)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    13321..13342
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(13630..14218)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(14389..15249)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(15250..15354)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     15828..15844
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agccgcacgt gctcggcgcg
     2341 gtcctgtact ggctgctgct gccctgcgcg ctgctggctg cctgcctgct ccgcttcagc
     2401 ggactctcgc tggtctacct gctcttcctg ctgctgctgc cctggttccc cggccccacc
     2461 cgatgcggcc tccaaggtca cacaggccgc ctcctgcggg cattgctggg cctcagcctg
     2521 ctcttcctgg tggcccatct cgccctccag atctgcctgc atattgtgcc ccgcctggac
     2581 cagctcctgg gacccagctg cagccgctgg gagaccctct cgcgacacat aggggtcaca
     2641 aggctggacc tgaaggacat ccccaacgcc atccggctgg tggcccctga cctgggcatc
     2701 ttggtggtct cctctgtctg cctcggcatc tgcgggcgcc ttgcaaggaa cacccggcag
     2761 agcccacatc cacgggagct ggatgatgat gagagggatg tggatgccag cccgacggca
     2821 gggctgcagg aagcagcaac gctggcccct acacggaggt cacggctggc cgctcgtttc
     2881 cgagtcacgg cccactggct gctggtggcg gctgggcggg tcctggccgt aacactgctt
     2941 gcactggcag gcatcgccca cccctcggcc ctctccagtg tctacctgct gctcttcctg
     3001 gccctctgca cctggtgggc ctgccacttt cccatcagca ctcggggctt cagcagactc
     3061 tgcgtcgcgg tggggtgctt cggcgccggc catctcatct gcctctactg ctaccagatg
     3121 cccttggcac aggctctgct cccgcctgcc ggcatctggg ctagggtgct gggtctcaag
     3181 gacttcgtgg gtcccaccaa ctgctccagc ccccacgcgc tggtcctcaa caccggcctg
     3241 gactggcctg tgtatgccag ccccggcgtc ctcctgctgc tgtgctacgc cacggcctct
     3301 ctgcgcaagc tccgcgcgta ccgcccctcc ggccagagga aggaggcggc aaaggggtat
     3361 gaggctcggg agctggagct agcagagctg gaccagtggc cccaggaacg ggagtctgac
     3421 cagcacgtgg tgcccacagc acccgacacc gaggctgata actgcatcgt gcacgagctg
     3481 accggccaga gctccgtcct gcggcggcct gtgcggccca agcgggctga gcccagggag
     3541 gcgtctccgc tccacagcct gggccacctc atcatggacc agagctatgt gtgcgcgctc
     3601 attgccatga tggtatggag catcacctac cacagctggc tgaccttcgt actgctgctc
     3661 tgggcctgcc tcatctggac ggtgcgcagc cgccaccaac tggccatgct gtgctcgccc
     3721 tgcatcctgc tgtatgggat gacgctgtgc tgcctacgct acgtgtgggc catggacctg
     3781 cgccctgagc tgcccaccac cctgggcccc gtcagcctgc gccagctggg gctggagcac
     3841 acccgctacc cctgtctgga ccttggtgcc atgttgctct acaccctgac cttctggctc
     3901 ctgctgcgcc agtttgtgaa agagaagctg ctgaagtggg cagagtctcc agctgcgctg
     3961 acggaggtca ccgtggcaga cacagagccc acgcggacgc agacgctgtt gcagagcctg
     4021 ggggagctgg tgaagggcgt gtacgccaag tactggatct atgtgtgtgc tggcatgttc
     4081 atcgtggtca gcttcgccgg ccgcctcgtg gtctacaaga ttgtctacat gttcctcttc
     4141 ctgctctgcc tcaccctctt ccaggtctac tacagcctgt ggcggaagct gctcaaggcc
     4201 ttctggtggc tcgtggtggc ctacaccatg ctggtcctca tcgccgtcta caccttccag
     4261 ttccaggact tccctgccta ctggcgcaac ctcactggct tcaccgacga gcagctgggg
     4321 gacctgggcc tggagcagtt cagcgtgtcc gagctcttct ccagcatcct ggtgcccggc
     4381 ttcttcctcc tggcctgcat cctgcagctg cactacttcc acaggccctt catgcagctc
     4441 accgacatgg agcacgtgtc cctgcctggc acgcgcctcc cgcgctgggc tcacaggcag
     4501 gatgcagtga gtgggacccc actgctgcgg gaggagcagc aggagcatca gcagcagcag
     4561 caggaggagg aggaggagga ggaggactcc agggacgagg ggctgggcgt ggccactccc
     4621 caccaggcca cgcaggtgcc tgaaggggca gccaagtggg gcctggtggc tgagcggctg
     4681 ctggagctgg cagccggctt ctcggacgtc ctctcacgcg tgcaggtgtt cctgcggcgg
     4741 ctgctggagc ttcacgtttt caagctggtg gccctgtaca ccgtctgggt ggccctgaag
     4801 gaggtgtcgg tgatgaacct gctgctggtg gtgctgtggg ccttcgccct gccctaccca
     4861 cgcttccggc ccatggcctc ctgcctgtcc accgtgtgga cctgcgtcat catcgtgtgt
     4921 aagatgctgt accagctcaa ggttgtcaac ccccaggagt attccagcaa ctgcaccgag
     4981 cccttcccca acagcaccaa cttgctgccc acggagatca gccagtccct gctgtaccgg
     5041 gggcccgtgg accctgccaa ctggtttggg gtgcggaaag ggttccccaa cctgggctac
     5101 atccagaacc acctgcaagt gctgctgctg ctggtattcg aggccatcgt gtaccggcgc
     5161 caggagcact accgccggca gcaccagctg gccccgctgc ctgcccaggc cgtgtttgcc
     5221 agcggcaccc gccagcagct ggaccaggat ctgctcggct gcctcaagta cttcatcaac
     5281 ttcttcttct acaaattcgg gctggagatc tgcttcctga tggccgtgaa cgtgatcggg
     5341 cagcgcatga actttctggt gaccctgcac ggttgctggc tggtggccat cctcacccgc
     5401 aggcaccgcc aggccattgc ccgcctctgg cccaactact gcctcttcct ggcgctgttc
     5461 ctgctgtacc agtacctgct gtgcctgggg atgcccccgg ccctgtgcat tgattatccc
     5521 tggcgctgga gccgggccgt ccccatgaac tccgcactca tcaagtggct gtacctgcct
     5581 gatttcttcc gggcccccaa ctccaccaac ctcatcagcg actttctcct gctgctgtgc
     5641 gcctcccagc agtggcaggt gttctcagct gagcgcacag aggagtggca gcgcatggct
     5701 ggcgtcaaca ccgaccgcct ggagccgctg cggggggagc ccaaccccgt gcccaacttt
     5761 atccactgca ggtcctacct tgacatgctg aaggtggccg tcttccgata cctgttctgg
     5821 ctggtgctgg tggtggtgtt tgtcacgggg gccacccgca tcagcatctt cgggctgggc
     5881 tacctgctgg cctgcttcta cctgctgctc ttcggcacgg ccctgctgca gagggacaca
     5941 cgggcccgcc tcgtgctgtg ggactgcctc attctgtaca acgtcaccgt catcatctcc
     6001 aagaacatgc tgtcgctcct ggcctgcgtc ttcgtggagc agatgcagac cggcttctgc
     6061 tgggtcatcc agctcttcag ccttgtatgc accgtcaagg gctactatga ccccaaggag
     6121 atgatggaca gagaccagga ctgcctgctg cctgtggagg aggctggcat catctgggac
     6181 agcgtctgct tcttcttcct gctgctgcag cgccgcgtct tccttagcca ttactacctg
     6241 cacgtcaggg ccgacctcca ggccaccgcc ctgctagcct ccaggggctt cgccctctac
     6301 aacgctgcca acctcaagag cattgacttt caccgcagga tagaggagaa gtccctggcc
     6361 cagctgaaaa gacagatgga gcgtatccgt gccaagcagg agaagcacag gcagggccgg
     6421 gtggaccgca gtcgccccca ggacaccctg ggccccaagg accccggcct ggagccaggg
     6481 cccgacagtc cagggggctc ctccccgcca cggaggcagt ggtggcggcc ctggctggac
     6541 cacgccacag tcatccactc cggggactac ttcctgtttg agtccgacag tgaggaagag
     6601 gaggaggctg ttcctgaaga cccgaggccg tcggcacaga gtgccttcca gctggcgtac
     6661 caggcatggg tgaccaacgc ccaggcggtg ctgaggcggc ggcagcagga gcaggagcag
     6721 gcaaggcagg aacaggcagg acagctaccc acaggaggtg gtcccagcca ggaggtggag
     6781 ccagcagagg gccccgagga ggcagcggca ggccggagcc atgtggtgca gagggtgctg
     6841 agcacggcgc agttcctgtg gatgctgggg caggcgctag tggatgagct gacacgctgg
     6901 ctgcaggagt tcacccggca ccacggcacc atgagcgacg tgctgcgggc agagcgctac
     6961 ctcctcacac aggagctcct gcagggcggc gaagtgcaca ggggcgtgct ggatcagctg
     7021 tacacaagcc aggccgaggc cacgctgcca ggccccaccg aggcccccaa tgccccaagc
     7081 accgtgtcca gtgggctggg cgcggaggag ccactcagca gcatgacaga cgacatgggc
     7141 agccccctga gcaccggcta ccacacgcgc agtggcagtg aggaggcagt caccgacccc
     7201 ggggagcgtg aggctggtgc ctctctgtac cagggactga tgcggacggc cagcgagctg
     7261 ctcctggaca ggcgcctgcg catcccagag ctggaggagg cagagctgtt tgcggagggg
     7321 cagggccggg cgctgcggct gctgcgggcc gtgtaccagt gtgtggccgc ccactcggag
     7381 ctgctctgct acttcatcat catcctcaac cacatggtca cggcctccgc cggctcgctg
     7441 gtgctgcccg tgctcgtctt cctgtgggcc atgctgtcga tcccgaggcc cagcaagcgc
     7501 ttctggatga cggccatcgt cttcaccgag atcgcggtgg tcgtcaagta cctgttccag
     7561 tttgggttct tcccctggaa cagccacgtg gtgctgcggc gctacgagaa caagccctac
     7621 ttcccgcccc gcatcctggg cctggagaag actgacggct acatcaagta cgacctggtg
     7681 cagctcatgg cccttttctt ccaccgctcc cagctgctgt gctatggcct ctgggaccat
     7741 gaggaggact caccatccaa ggagcatgac aagagcggcg aggaggagca gggagccgag
     7801 gaggggccag gggtgcctgc ggccaccacc gaagaccaca ttcaggtgga agccagggtc
     7861 ggacccacgg acgggacccc agaaccccaa gtggagctca ggccccgtga tacgaggcgc
     7921 atcagtctac gttttagaag aaggaagaag gagggcccag cacggaaagg agcggcagcc
     7981 atcgaagctg aggacaggga ggaagaagag ggggaggaag agaaagaggc ccccacgggg
     8041 agagagaaga ggccaagccg ctctggagga agagtaaggg cggccgggcg gcggctgcag
     8101 ggcttctgcc tgtccctggc ccagggcaca tatcggccgc tacggcgctt cttccacgac
     8161 atcctgcaca ccaagtaccg cgcagccacc gacgtctatg ccctcatgtt cctggctgat
     8221 gttgtcgact tcatcatcat catttttggc ttctgggcct ttgggaagca ctcggcggcc
     8281 acagacatca cgtcctccct atcagacgac caggtacccg aggctttcct ggtcatgctg
     8341 ctgatccagt tcagtaccat ggtggttgac cgcgccctct acctgcgcaa gaccgtgctg
     8401 ggcaagctgg ccttccaggt ggcgctggtg ctggccatcc acctatggat gttcttcatc
     8461 ctgcccgccg tcactgagag gatgttcaac cagaatgtgg tggcccagct ctggtacttc
     8521 gtgaagtgca tctacttcgc cctgtccgcc taccagatcc gctgcggcta ccccacccgc
     8581 atcctcggca acttcctcac caagaagtac aatcatctca acctcttcct cttccagggg
     8641 ttccggctgg tgccgttcct ggtggagctg cgggcagtga tggactgggt gtggacggac
     8701 accacgctgt ccctgtccag ctggatgtgt gtggaggaca tctatgccaa catcttcatc
     8761 atcaaatgca gccgagagac agagaagaaa tacccgcagc ccaaagggca gaagaagaag
     8821 aagatcgtca agtacggcat gggtggcctc atcatcctct tcctcatcgc catcatctgg
     8881 ttcccactgc tcttcatgtc gctggtgcgc tccgtggttg gggttgtcaa ccagcccatc
     8941 gatgtcaccg tcaccctgaa gctgggcggc tatgagccgc tgttcaccat gagcgcccag
     9001 cagccgtcca tcatcccctt cacggcccag gcctatgagg agctgtcccg gcagtttgac
     9061 ccccagccgc tggccatgca gttcatcagc cagtacagcc ctgaggacat cgtcacggcg
     9121 cagattgagg gcagctccgg ggcgctgtgg cgcatcagtc cccccagccg tgcccagatg
     9181 aagcgggagc tctacaacgg cacggccgac atcaccctgc gcttcacctg gaacttccag
     9241 agggacctgg cgaagggagg cactgtggag tatgccaacg agaagcacat gctggccctg
     9301 gcccccaaca gcactgcacg gcggcagctg gccagcctgc tcgagggcac ctcggaccag
     9361 tctgtggtca tccctaatct cttccccaag tacatccgtg cccccaacgg gcccgaagcc
     9421 aaccctgtga agcagctgca gcccaatgag gaggccgact acctcggcgt gcgtatccag
     9481 ctgcggaggg agcagggtgc gggggccacc ggcttcctcg aatggtgggt catcgagctg
     9541 caggagtgcc ggaccgactg caacctgctg cccatggtca ttttcagtga caaggtcagc
     9601 ccaccgagcc tcggcttcct ggctggctac ggcatcatgg ggctgtacgt gtccatcgtg
     9661 ctggtcatcg gcaagttcgt gcgcggattc ttcagcgaga tctcgcactc cattatgttc
     9721 gaggagctgc cgtgcgtgga ccgcatcctc aagctctgcc aggacatctt cctggtgcgg
     9781 gagactcggg agctggagct ggaggaggag ttgtacgcca agctcatctt cctctaccgc
     9841 tcaccggaga ccatgatcaa gtggactcgt gagaaggagg gatccgacta caaagaccat
     9901 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     9961 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
    10021 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
    10081 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
    10141 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
    10201 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
    10261 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
    10321 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
    10381 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
    10441 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
    10501 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
    10561 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
    10621 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
    10681 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
    10741 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
    10801 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
    10861 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
    10921 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
    10981 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
    11041 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
    11101 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
    11161 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
    11221 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
    11281 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
    11341 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
    11401 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
    11461 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
    11521 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
    11581 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
    11641 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
    11701 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
    11761 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
    11821 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
    11881 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
    11941 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
    12001 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
    12061 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
    12121 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
    12181 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
    12241 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
    12301 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
    12361 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
    12421 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
    12481 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
    12541 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
    12601 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
    12661 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    12721 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    12781 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
    12841 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
    12901 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    12961 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    13021 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
    13081 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    13141 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
    13201 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
    13261 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    13321 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    13381 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    13441 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    13501 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    13561 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    13621 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    13681 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    13741 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    13801 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    13861 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    13921 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    13981 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    14041 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    14101 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    14161 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    14221 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    14281 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    14341 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    14401 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    14461 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    14521 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    14581 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    14641 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    14701 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    14761 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    14821 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    14881 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    14941 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    15001 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    15061 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    15121 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    15181 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    15241 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    15301 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    15361 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    15421 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    15481 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    15541 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    15601 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    15661 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    15721 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    15781 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    15841 cagtgccaag ctg
//
