LOCUS       V031692                10274 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031692
VERSION     V031692
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4275
                     /label="PIAS1(NM_001320687)"
                     /note="PIAS1(NM_001320687)"
                     /gene="PIAS1"
     CDS             4282..4308
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4312..4338
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4342..4368
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4433..4644
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4657..4925
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4954..4963
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4984..5646
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5716..5769
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5770..6369
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6370..6957
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7031..7264
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7336..7457
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7476..7611
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7649..7665)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7673..7689
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7697..7727)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7742..7763
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8051..8639)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8810..9670)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9671..9775)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10249..10265
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt ttacactcca agattcgtat
     2341 gttaagcaaa tggttatgag ccttagagtt tctgaactcc aagtactgtt gggctacgcc
     2401 gggagaaaca agcacggacg caaacacgaa cttctcacaa aagccctgca tttgctaaag
     2461 gctggctgta gtcctgctgt gcaaatgaaa attaaggaac tctataggcg gcggttccca
     2521 cagaaaatca tgacgcctgc agacttgtcc atccccaacg tacattcaag tcctatgcca
     2581 gcaactttgt ctccatctac cattccacaa ctcacttacg atggtcaccc tgcatcatcg
     2641 ccattactcc ctgtttctct tctgggacct aaacatgaac tggaactccc acatcttaca
     2701 tcagctcttc acccagtcca tccggatata aaacttcaaa aattaccatt ttatgattta
     2761 ctggatgaac tgataaaacc caccagtcta gcatcagaca acagtcagcg ctttcgagaa
     2821 acctgttttg catttgcctt gacaccacaa caagtgcagc aaatcagtag ttccatggat
     2881 atttctggga ccaaatgtga cttcacagta caggtccagt taaggttttg tttatcagaa
     2941 accagttgtc cacaagaaga tcacttccca cccaatcttt gtgtgaaagt gaatacaaaa
     3001 ccttgcagcc ttccaggtta ccttccacct acaaaaaatg gcgtggaacc aaagcgaccc
     3061 agccgaccaa ttaatatcac ctcacttgtc cgactgtcca caacagtacc aaacacgatt
     3121 gttgtttctt ggactgcaga aattggaaga aactattcca tggcagtata tcttgtaaaa
     3181 cagttgtcct caacagttct tcttcagagg ttacgagcaa agggaataag gaatccggat
     3241 cattctagag ctttaattaa agagaagttg actgcggatc cagacagtga aatagctaca
     3301 accagcctaa gggtttctct actatgtcca cttggtaaaa tgcggctgac aattccgtgt
     3361 cgggccctta catgttctca tctacaatgt tttgacgcaa ctctttacat tcagatgaat
     3421 gagaaaaaac caacctgggt ttgtcctgtc tgtgataaga aggctccata tgaacacctt
     3481 attattgatg gcttgtttat ggaaatccta aagtactgta cagactgtga tgaaatacaa
     3541 tttaaggagg atggcacttg ggcaccgatg agatcaaaaa aggaagtaca ggaagtttct
     3601 gcctcttaca atggagtcga tggatgcttg agctccacat tggagcatca ggtagcgtct
     3661 caccaccagt cctcaaataa aaacaagaaa gtagaagtga ttgacctaac catagacagt
     3721 tcatctgatg aagaggaaga agagccatct gccaagagga cctgtccttc cctatctccc
     3781 acatcaccac taaataataa aggcatttta agtcttccac atcaagcatc tccagtatcc
     3841 cgcaccccaa gccttcctgc tgtagacaca agctacatta atacctccct catccaagac
     3901 tataggcatc ctttccacat gacacccatg ccttacgact tacaaggatt agatttcttt
     3961 cctttcttat caggagacaa tcagcattac aacacctcct tgcttgccgc tgcagcagca
     4021 gcagtttcag atgatcaaga cctcctacac tcgtctcggt ttttcccgta tacctcctca
     4081 cagatgtttc ttgatcagtt aagtgcagga ggcagtactt ctctgccaac caccaatgga
     4141 agcagtagtg gcagtaacag cagcctggtt tcttccaaca gcctaaggga aagccatagc
     4201 cacaccgtca caaacaggag cagcacggac acggcatcca tctttggcat cataccagac
     4261 attatttcat tggacctcga gtacccatac gacgtacctg attatgcaac ttatccttat
     4321 gatgtacctg attatgctag ctatccttat gatgtcccag actacgcata gggatccctg
     4381 cgatatccag cgcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     4441 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     4501 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     4561 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     4621 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     4681 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     4741 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     4801 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     4861 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     4921 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc
     4981 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     5041 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     5101 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     5161 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     5221 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     5281 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     5341 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     5401 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     5461 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     5521 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     5581 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
     5641 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     5701 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     5761 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     5821 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     5881 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     5941 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6001 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6061 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6121 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6181 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6241 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     6301 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     6361 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6421 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6481 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6541 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6601 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6661 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6721 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     6781 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6841 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6901 ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg
     6961 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7021 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt
     7081 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7141 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7201 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7261 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7321 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7381 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7441 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     7501 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7561 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
     7621 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7681 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7741 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7801 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7861 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7921 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7981 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8041 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8101 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8161 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8221 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8281 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8341 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8401 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8461 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8521 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8581 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8641 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8701 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8761 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8821 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8881 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8941 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9001 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9061 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9121 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9181 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9241 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9301 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9361 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9421 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9481 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9541 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9601 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9661 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9721 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9781 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     9841 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     9901 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     9961 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    10021 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    10081 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
    10141 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    10201 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    10261 ccagtgccaa gctg
//
