LOCUS       V043810                10721 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043810
VERSION     V043810
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4806
                     /label="PFKL(NM_001002021)"
                     /note="PFKL(NM_001002021)"
                     /gene="PFKL"
     CDS             4813..4836
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHH"
                     /label="8xHis"
     promoter        4880..5091
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5104..5372
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5401..5410
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5431..6093
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6163..6216
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6217..6816
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6817..7404
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7478..7711
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7783..7904
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7923..8058
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8096..8112)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8120..8136
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8144..8174)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8189..8210
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8498..9086)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9257..10117)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10118..10222)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10696..10712
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga gaagcatgtg taaccagggt
     2341 agaggtcgag agtcctctcg tgggggtctc catgttcaag ggagctgccg aggcttgagc
     2401 aggagccccc agcaggaaac tggctttgcc aaggcccccg ctgggacaga ctgtttcttt
     2461 cactgcagtc ctgggagccg agggcaaggg gacaggaaag aggaagtgac ctcagagcct
     2521 ggtggcacca gcatcatgtc caggctgggg ggcatgaacg ctgctgtccg ggctgtgacg
     2581 cgcatgggca tttatgtggg tgccaaagtc ttcctcatct acgagggcta tgagggcctc
     2641 gtggagggag gtgagaacat caagcaggcc aactggctga gcgtctccaa catcatccag
     2701 ctgggcggca ctatcattgg cagcgctcgc tgcaaggcct ttaccaccag ggaggggcgc
     2761 cgggcagcgg cctacaacct ggtccagcac ggcatcacca acctgtgcgt catcggcggg
     2821 gatggcagcc tcacaggtgc caacatcttc cgcagcgagt ggggcagcct gctggaggag
     2881 ctggtggcgg aaggtaagat ctcagagact acagcccgga cctactcgca cctgaacatc
     2941 gcgggcctag tgggctccat cgataacgac ttctgcggca ccgacatgac catcggcacg
     3001 gactcggccc tccaccgcat catggaggtc atcgatgcca tcaccaccac tgcccagagc
     3061 caccagagga ccttcgtgct ggaagtgatg ggccggcact gcgggtacct ggcgctggta
     3121 tctgcactgg cctcaggggc cgactggctg ttcatccccg aggctccacc cgaggacggc
     3181 tgggagaact tcatgtgtga gaggctgggt gagactcgga gccgtgggtc ccgactgaac
     3241 atcatcatca tcgctgaggg tgccattgac cgcaacggga agcccatctc gtccagctac
     3301 gtgaaggacc tggtggttca gaggctgggc ttcgacaccc gtgtaactgt gctgggccac
     3361 gtgcagcggg gagggacgcc ctctgccttc gaccggatcc tgagcagcaa gatgggcatg
     3421 gaggcggtga tggcgctgct ggaagccacg cctgacacgc cggcctgcgt ggtcaccctc
     3481 tcggggaacc agtcagtgcg gctgcccctc atggagtgcg tgcagatgac caaggaagtg
     3541 cagaaagcca tggatgacaa gaggtttgac gaggccaccc agctccgtgg tgggagcttc
     3601 gagaacaact ggaacattta caagctcctc gcccaccaga agccccccaa ggagaagtct
     3661 aacttctccc tggccatcct gaatgtgggg gccccggcgg ctggcatgaa tgcggccgtg
     3721 cgctcggcgg tgcggaccgg catctcccat ggacacacag tatacgtggt gcacgatggc
     3781 ttcgaaggcc tagccaaggg tcaggtgcaa gaagtaggct ggcacgacgt ggccggctgg
     3841 ttggggcgtg gtggctccat gctggggacc aagaggaccc tgcccaaggg ccagctggag
     3901 tccattgtgg agaacatccg catctatggt attcacgccc tgctggtggt cggtgggttt
     3961 gaggcctatg aaggggtgct gcagctggtg gaggctcgcg ggcgctacga ggagctctgc
     4021 atcgtcatgt gtgtcatccc agccaccatc agcaacaacg tccctggcac cgacttcagc
     4081 ctgggctccg acactgctgt aaatgccgcc atggagagct gtgaccgcat caaacagtct
     4141 gcctcgggga ccaagcgccg tgtgttcatc gtggagacca tggggggtta ctgtggctac
     4201 ctggccaccg tgactggcat tgctgtgggg gccgacgccg cctacgtctt cgaggaccct
     4261 ttcaacatcc acgacttaaa ggtcaacgtg gagcacatga cggagaagat gaagacagac
     4321 attcagaggg gcctggtgct gcggaacgag aagtgccatg actactacac cacggagttc
     4381 ctgtacaacc tgtactcatc agagggcaag ggcgtcttcg actgcaggac caatgtcctg
     4441 ggccacctgc agcagggtgg cgctccaacc ccctttgacc ggaactatgg gaccaagctg
     4501 ggggtgaagg ccatgctgtg gttgtcggag aagctgcgcg aggtttaccg caagggacgg
     4561 gtgttcgcca atgccccaga ctcggcctgc gtgatcggcc tgaagaagaa ggcggtggcc
     4621 ttcagccccg tcactgagct caagaaagac actgatttcg agcaccgcat gccacgggag
     4681 cagtggtggc tgagcctgcg gctcatgctg aagatgctgg cacaataccg catcagtatg
     4741 gccgcctacg tgtcagggga gctggagcac gtgacccgcc gcaccctgag catggacaag
     4801 ggcttcggat cccaccatca ccatcaccat caccattagc ggccgcgaag gatctgcgat
     4861 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     4921 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     4981 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     5041 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     5101 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     5161 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     5221 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     5281 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     5341 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     5401 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     5461 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     5521 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     5581 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     5641 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     5701 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     5761 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     5821 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     5881 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     5941 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     6001 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     6061 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     6121 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     6181 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     6241 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     6301 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     6361 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6421 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     6481 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     6541 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6601 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6661 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6721 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6781 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     6841 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6901 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6961 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7021 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7081 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7141 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7201 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7261 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7321 ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg
     7381 atctcccttt ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     7441 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7501 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     7561 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7621 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7681 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     7741 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     7801 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7861 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     7921 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7981 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8041 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     8101 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8161 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8221 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8281 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8341 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8401 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8461 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8521 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8581 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8641 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8701 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8761 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8821 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8881 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     8941 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9001 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9061 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9121 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9181 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9241 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9301 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9361 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9421 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9481 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9541 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9601 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9661 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9721 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9781 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9841 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9901 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9961 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10021 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10081 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10141 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10201 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    10261 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    10321 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    10381 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    10441 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    10501 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
    10561 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    10621 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    10681 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
