LOCUS       V046039                10909 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046039
VERSION     V046039
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5763
                     /label="PDZD8(NM_173791)"
                     /note="PDZD8(NM_173791)"
                     /gene="PDZD8"
     CDS             5770..5835
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5879..6090
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6103..6371
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6398..6997
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7004..7592
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7666..7899
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7971..8092
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8111..8246
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8284..8300)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8308..8324
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8332..8362)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8377..8398
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8686..9274)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9445..10305)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10306..10410)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10884..10900
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggggctg ctgctcatga tcctggcgtc ggccgtgctg
     2341 ggttccttcc tcacgctcct cgcccagttc ttcctgctgt accgcagaca gcccgagccg
     2401 ccggcggacg aggccgcccg cgcgggcgag ggcttccgct acatcaagcc agtgccgggc
     2461 ctgctcctaa gggagtacct ttatggcggc ggccgggatg aggagccctc cggagcggcc
     2521 cctgagggcg gcgcgacccc caccgcggcc cccgagaccc ccgccccgcc gacgcgggag
     2581 acttgctact tcctcaacgc caccatccta ttcctgttcc gggagttgcg ggacaccgcg
     2641 ctgacccgcc gctgggtcac caagaagatc aaggtggagt tcgaggagct gctgcagacc
     2701 aagacggccg ggcgcctgct ggaggggctg agcctgcggg acgtgttcct gggcgagacg
     2761 gtgcccttca tcaagaccat ccggctcgtg cggccagtcg tgccctcggc caccggggag
     2821 cccgatggcc ctgaagggga ggcgctgccc gccgcctgcc ccgaggagct ggccttcgag
     2881 gcggaggtgg agtacaacgg gggcttccac ctggccatcg acgtggacct ggtcttcggc
     2941 aagtccgcct acttgtttgt caagctgtcc cgcgtggtgg gaaggctgcg cttggtcttt
     3001 acgcgcgtgc ccttcaccca ctggttcttc tccttcgtgg aagacccgct gatcgacttc
     3061 gaggtgcgct cccagtttga agggcggccc atgccccagc tcacctccat catcgtcaac
     3121 cagctcaaga agatcatcaa gcgcaagcac accctaccga attacaagat caggtttaag
     3181 ccgttttttc cataccagac cttgcaagga tttgaagaag atgaagagca tatccatata
     3241 caacaatggg cacttactga aggccgtctt aaagttacgt tgttagaatg tagcaggtta
     3301 ctcatttttg gatcctatga cagagaggca aatgttcatt gcacacttga gttaagcagt
     3361 agtgtttggg aagaaaaaca gaggagttct attaagacgg ttgaattaat aaaaggaaat
     3421 ttacaaagtg ttggacttac acttcgtctt gtccagtcaa ctgatgggta tgctgggcac
     3481 gtcatcattg aaactgtggc tccaaactcg cctgctgcaa ttgcagatct tcagcgggga
     3541 gatcgactta tcgccattgg aggtgtgaaa atcacatcaa cactgcaagt gttgaagctt
     3601 atcaagcagg ctggtgaccg agtcctggtg tactatgaaa ggcctgttgg ccagagtaat
     3661 caaggtgcag tgctgcaaga taactttggc cagttggaag aaaacttttt gtcaagctca
     3721 tgccaatcgg gttatgaaga ggaagctgcc gggttgacag tagatactga aagtagagag
     3781 ctggattctg aatttgaaga cttggcaagt gatgtcagag cacaaaatga gttcaaagat
     3841 gaggcacaat cattaagtca tagtcccaaa cgtgttccaa caacactttc tattaaaccc
     3901 cttggagcta tatcaccagt tttaaaccgt aaattagctg taggaagtca cccactacca
     3961 ccgaaaattc agtccaaaga tggaaataaa cctccacccc taaaaacttc tgagataaca
     4021 gacccagcac aagtgtcaaa accaacccaa ggatctgctt tcaaaccacc tgtgccacca
     4081 cgaccacaag cgaaagttcc tttgccttcc gccgatgctc caaatcaggc agaaccagat
     4141 gttctcgttg aaaagccaga gaaggtggtg ccacctcctc ttgtagataa atctgctgaa
     4201 aagcaagcaa aaaatgtgga tgccatagac gatgcagctg cacctaagca atttttagca
     4261 aagcaagaag tggccaaaga tgtcacttca gaaacttcct gccctactaa ggacagttcg
     4321 gacgaccgtc aaacatggga atcatcagaa attctttatc gtaataagct aggaaaatgg
     4381 acaagaacca gagcatcctg tttgtttgac atagaagcct gtcacaggta cttaaacatt
     4441 gcattgtggt gcagggatcc tttcaagttg ggaggtctca tctgtttggg gcatgttagt
     4501 ttaaaacttg aagatgtggc tttaggatgc ctagctacat caaacacgga atacctttcc
     4561 aaattgagac tggaagcccc ctcacctaag gctatagtca ctagaaccgc actacgcaat
     4621 ctgagtatgc aaaagggatt caatgacaaa ttttgctatg gtgacattac tattcacttc
     4681 aaatatttga aagaaggaga atcagaccac catgtagtta ctaacgtaga aaaagaaaaa
     4741 gaaccccatt tggttgaaga agtttctgtt ctccctaaag aggagcaatt tgttggacag
     4801 atgggtttaa cagaaaacaa acacagtttt caggatactc agttccagaa cccaacatgg
     4861 tgtgactact gtaagaaaaa agtttggact aaagcagctt cccagtgtat gttttgtgct
     4921 tatgtttgcc ataaaaaatg tcaagaaaag tgtctagctg agacttctgt ttgtggagca
     4981 actgataggc gaatagacag gacactgaaa aaccttaggc tggaaggaca ggaaaccctc
     5041 ttaggcctgc ctcctcgtgt tgatgctgaa gctagcaagt cagtcaataa aacaacaggt
     5101 ttgacaaggc atattatcaa tactagttct cgtttattaa atttgcgtca agtctctaaa
     5161 actcgccttt ctgaaccagg aaccgatctc gtagaacctt caccaaaaca cacacccaac
     5221 acgtcagaca acgaaggcag tgacacggag gtctgtggtc caaacagtcc ttctaaacgg
     5281 ggaaacagca caggaataaa gttagtgaga aaagagggtg gtctggatga cagtgttttc
     5341 attgcagtta aagaaattgg tcgtgatctg tacaggggct tgcctacaga ggaaaggatc
     5401 cagaaactag agttcatgtt ggataagcta cagaatgaaa ttgatcagga gttggaacac
     5461 aataattccc ttgttagaga agaaaaagag acaactgata caaggaaaaa atcacttctt
     5521 tctgctgcct tagctaaatc aggtgaaagg ctacaagctc taacacttct tatgattcac
     5581 tacagagcag gcattgaaga tatagaaact ttagaaagtc tgtctttaga ccagcactcc
     5641 aaaaaaataa gcaagtacac agatgataca gaagaagacc ttgataatga aataagccaa
     5701 ctaatagact ctcagccatt cagcagcata tcagatgact tatttggccc atccgagtct
     5761 gtgggatccg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     5821 gatgacgatg acaagtagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     5881 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     5941 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     6001 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     6061 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     6121 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     6181 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     6241 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     6301 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     6361 cgttacagat ccaagctgtg accggcgcct acgctagatg accgagtaca agcccacggt
     6421 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
     6481 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
     6541 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6601 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6661 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6721 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6781 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6841 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6901 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6961 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtc gacaatcaac ctctggatta
     7021 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7081 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7141 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7201 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7261 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7321 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7381 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     7441 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7501 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7561 gagtcggatc tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca
     7621 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7681 actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7741 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7801 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7861 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     7921 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     7981 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8041 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8101 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8161 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8221 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc
     8281 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8341 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8401 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8461 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8521 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8581 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8641 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8701 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8761 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8821 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8881 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8941 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9001 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9061 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9121 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9181 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9241 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9301 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9361 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9421 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9481 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9541 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9601 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9661 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9721 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9781 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9841 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9901 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9961 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10021 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10081 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10141 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10201 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10261 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10321 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10381 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10441 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    10501 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    10561 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    10621 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    10681 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    10741 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    10801 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    10861 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
