LOCUS V046754 9636 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046754 VERSION V046754 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..3831 /label="PAX7(NM_001135254)" /note="PAX7(NM_001135254)" /gene="PAX7" CDS 3838..3864 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3868..3894 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3898..3924 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3989..4200 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4213..4481 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 4510..4519 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 4540..5202 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5272..5325 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5326..5724 /codon_start=1 /gene="Aspergillus terreus BSD" /note="confers resistance to blasticidin" /product="blasticidin S deaminase" /transl_table=1 /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG*" /label="BSD" misc_feature 5731..6319 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6393..6626 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6698..6819 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6838..6973 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7011..7027) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7035..7051 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7059..7089) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7104..7125 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7413..8001) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8172..9032) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9033..9137) /gene="bla" /label="AmpR promoter" primer_bind 9611..9627 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggcccttcc cggcacggta 2341 ccgagaatga tgcggccggc tccggggcag aactaccccc gcacgggatt ccctttggaa 2401 gtgtccaccc cgcttggcca aggccgggtc aatcagctgg gaggggtctt catcaatggg 2461 cgacccctgc ctaaccacat ccgccacaag atagtggaga tggcccacca tggcatccgg 2521 ccctgtgtca tctcccgaca gctgcgtgtc tcccacggct gcgtctccaa gattctttgc 2581 cgctaccagg agaccgggtc catccggcct ggggccatcg gcggcagcaa gcccagacag 2641 gtggcgactc cggatgtaga gaaaaagatt gaggagtaca agagggaaaa cccaggcatg 2701 ttcagctggg agatccggga caggctgctg aaggatgggc actgtgaccg aagcactgtg 2761 ccctcaggtt tagtgagttc gattagccgc gtgctcagaa tcaagttcgg gaagaaagag 2821 gaggaggatg aagcggacaa gaaggaggac gacggcgaaa agaaggccaa acacagcatc 2881 gacggcatcc tgggcgacaa agggaaccgg ctggacgagg gctcggatgt ggagtcggaa 2941 cctgacctcc cactgaagcg caagcagcga cgcagtcgga ccacattcac ggccgagcag 3001 ctggaggagc tggagaaggc ctttgagagg acccactacc cagacatata cacccgcgag 3061 gagctggcgc agaggaccaa gctgacagag gcgcgtgtgc aggtctggtt cagtaaccgc 3121 cgcgcccgtt ggcgtaagca ggcaggagcc aaccagctgg cggcgttcaa ccaccttctg 3181 ccaggaggct tcccacccac cggcatgccc acgctgcccc cctaccagct gccggactcc 3241 acctacccca ccaccaccat ctcccaagat gggggcagca ctgtgcaccg gcctcagccc 3301 ctgccaccgt ccaccatgca ccagggcggg ctggctgcag cggctgcagc cgccgacacc 3361 agctctgcct acggagcccg ccacagcttc tccagctact ctgacagctt catgaatccg 3421 gcggcgccct ccaaccacat gaacccggtc agcaacggcc tgtctcctca ggtgatgagc 3481 atcttgggca accccagtgc ggtgcccccg cagccacagg ctgacttctc catctccccg 3541 ctgcatggcg gcctggactc ggccacctcc atctcagcca gctgcagcca gcgggccgac 3601 tccatcaagc caggagacag cctgcccacc tcccaggcct actgcccacc cacctacagc 3661 accaccggct acagcgtgga ccccgtggcc ggctatcagt acggccagta cggccagact 3721 gctgttgatt atctggccaa aaatgtgagc ctctccaccc agcgtcgcat gaagctcggg 3781 gagcactctg ctgtgctggg actcctgcct gtggaaactg gccaggccta cctcgagtac 3841 ccatacgacg tacctgatta tgcaacttat ccttatgatg tacctgatta tgctagctat 3901 ccttatgatg tcccagacta cgcataggga tccctgcgat atccagcgcg gccgcgaagg 3961 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 4021 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 4081 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 4141 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 4201 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 4261 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 4321 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 4381 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 4441 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 4501 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 4561 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 4621 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 4681 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 4741 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 4801 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 4861 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 4921 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 4981 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 5041 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 5101 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 5161 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 5221 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 5281 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatggc caagcctttg 5341 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc 5401 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt 5461 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct 5521 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc 5581 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa 5641 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc 5701 tctggttatg tgtgggaggg ctaagtcgac aatcaacctc tggattacaa aatttgtgaa 5761 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5821 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 5881 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 5941 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 6001 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 6061 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6121 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 6181 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6241 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 6301 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 6361 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa 6421 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6481 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6541 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6601 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6661 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 6721 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6781 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc 6841 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 6901 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 6961 ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg 7021 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 7081 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 7141 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 7201 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 7261 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 7321 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 7381 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 7441 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 7501 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 7561 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 7621 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 7681 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 7741 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 7801 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 7861 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 7921 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 7981 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 8041 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 8101 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 8161 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 8221 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 8281 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 8341 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 8401 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 8461 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 8521 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 8581 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 8641 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 8701 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 8761 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 8821 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 8881 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 8941 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 9001 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 9061 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 9121 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 9181 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt 9241 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 9301 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 9361 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 9421 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca 9481 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag 9541 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag 9601 tcacgacgtt gtaaaacgac ggccagtgcc aagctg //