LOCUS       V037582                11289 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037582
VERSION     V037582
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5344
                     /label="PARP1(NM_001618)"
                     /note="PARP1(NM_001618)"
                     /gene="PARP1"
     CDS             5351..5377
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5381..5407
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5411..5437
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5487..5698
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5711..5979
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6014..6721
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6731..6784
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6785..7384
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7385..7972
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8046..8279
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8351..8472
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8491..8626
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8664..8680)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8688..8704
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8712..8742)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8757..8778
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9066..9654)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9825..10685)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10686..10790)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11264..11280
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggcgga gtcttcggat aagctctatc gagtcgagta
     2341 cgccaagagc gggcgcgcct cttgcaagaa atgcagcgag agcatcccca aggactcgct
     2401 ccggatggcc atcatggtgc agtcgcccat gtttgatgga aaagtcccac actggtacca
     2461 cttctcctgc ttctggaagg tgggccactc catccggcac cctgacgttg aggtggatgg
     2521 gttctctgag cttcggtggg atgaccagca gaaagtcaag aagacagcgg aagctggagg
     2581 agtgacaggc aaaggccagg atggaattgg tagcaaggca gagaagactc tgggtgactt
     2641 tgcagcagag tatgccaagt ccaacagaag tacgtgcaag gggtgtatgg agaagataga
     2701 aaagggccag gtgcgcctgt ccaagaagat ggtggacccg gagaagccac agctaggcat
     2761 gattgaccgc tggtaccatc caggctgctt tgtcaagaac agggaggagc tgggtttccg
     2821 gcccgagtac agtgcgagtc agctcaaggg cttcagcctc cttgctacag aggataaaga
     2881 agccctgaag aagcagctcc caggagtcaa gagtgaagga aagagaaaag gcgatgaggt
     2941 ggatggagtg gatgaagtgg cgaagaagaa atctaaaaaa gaaaaagaca aggatagtaa
     3001 gcttgaaaaa gccctaaagg ctcagaacga cctgatctgg aacatcaagg acgagctaaa
     3061 gaaagtgtgt tcaactaatg acctgaagga gctactcatc ttcaacaagc agcaagtgcc
     3121 ttctggggag tcggcgatct tggaccgagt agctgatggc atggtgttcg gtgccctcct
     3181 tccctgcgag gaatgctcgg gtcagctggt cttcaagagc gatgcctatt actgcactgg
     3241 ggacgtcact gcctggacca agtgtatggt caagacacag acacccaacc ggaaggagtg
     3301 ggtaacccca aaggaattcc gagaaatctc ttacctcaag aaattgaagg ttaaaaaaca
     3361 ggaccgtata ttccccccag aaaccagcgc ctccgtggcg gccacgcctc cgccctccac
     3421 agcctcggct cctgctgctg tgaactcctc tgcttcagca gataagccat tatccaacat
     3481 gaagatcctg actctcggga agctgtcccg gaacaaggat gaagtgaagg ccatgattga
     3541 gaaactcggg gggaagttga cggggacggc caacaaggct tccctgtgca tcagcaccaa
     3601 aaaggaggtg gaaaagatga ataagaagat ggaggaagta aaggaagcca acatccgagt
     3661 tgtgtctgag gacttcctcc aggacgtctc cgcctccacc aagagccttc aggagttgtt
     3721 cttagcgcac atcttgtccc cttggggggc agaggtgaag gcagagcctg ttgaagttgt
     3781 ggccccaaga gggaagtcag gggctgcgct ctccaaaaaa agcaagggcc aggtcaagga
     3841 ggaaggtatc aacaaatctg aaaagagaat gaaattaact cttaaaggag gagcagctgt
     3901 ggatcctgat tctggactgg aacactctgc gcatgtcctg gagaaaggtg ggaaggtctt
     3961 cagtgccacc cttggcctgg tggacatcgt taaaggaacc aactcctact acaagctgca
     4021 gcttctggag gacgacaagg aaaacaggta ttggatattc aggtcctggg gccgtgtggg
     4081 tacggtgatc ggtagcaaca aactggaaca gatgccgtcc aaggaggatg ccattgagca
     4141 cttcatgaaa ttatatgaag aaaaaaccgg gaacgcttgg cactccaaaa atttcacgaa
     4201 gtatcccaaa aagttctacc ccctggagat tgactatggc caggatgaag aggcagtgaa
     4261 gaagctgaca gtaaatcctg gcaccaagtc caagctcccc aagccagttc aggacctcat
     4321 caagatgatc tttgatgtgg aaagtatgaa gaaagccatg gtggagtatg agatcgacct
     4381 tcagaagatg cccttgggga agctgagcaa aaggcagatc caggccgcat actccatcct
     4441 cagtgaggtc cagcaggcgg tgtctcaggg cagcagcgac tctcagatcc tggatctctc
     4501 aaatcgcttt tacaccctga tcccccacga ctttgggatg aagaagcctc cgctcctgaa
     4561 caatgcagac agtgtgcagg ccaaggtgga aatgcttgac aacctgctgg acatcgaggt
     4621 ggcctacagt ctgctcaggg gagggtctga tgatagcagc aaggatccca tcgatgtcaa
     4681 ctatgagaag ctcaaaactg acattaaggt ggttgacaga gattctgaag aagccgagat
     4741 catcaggaag tatgttaaga acactcatgc aaccacacac aatgcgtatg acttggaagt
     4801 catcgatatc tttaagatag agcgtgaagg cgaatgccag cgttacaagc cctttaagca
     4861 gcttcataac cgaagattgc tgtggcacgg gtccaggacc accaactttg ctgggatcct
     4921 gtcccagggt cttcggatag ccccgcctga agcgcccgtg acaggctaca tgtttggtaa
     4981 agggatctat ttcgctgaca tggtctccaa gagtgccaac tactgccata cgtctcaggg
     5041 agacccaata ggcttaatcc tgttgggaga agttgccctt ggaaacatgt atgaactgaa
     5101 gcacgcttca catatcagca agttacccaa gggcaagcac agtgtcaaag gtttgggcaa
     5161 aactacccct gatccttcag ctaacattag tctggatggt gtagacgttc ctcttgggac
     5221 cgggatttca tctggtgtga atgacacctc tctactatat aacgagtaca ttgtctatga
     5281 tattgctcag gtaaatctga agtatctgct gaaactgaaa ttcaatttta agacctccct
     5341 gtggctcgag tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga
     5401 ttatgctagc tatccttatg atgtcccaga ctacgcatag gatccgcggc cgcgaaggat
     5461 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     5521 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     5581 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     5641 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     5701 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     5761 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     5821 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     5881 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     5941 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     6001 gctagacgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     6061 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     6121 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     6181 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     6241 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     6301 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     6361 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     6421 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     6481 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     6541 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     6601 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     6661 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     6721 gggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     6781 ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     6841 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     6901 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     6961 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     7021 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     7081 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     7141 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     7201 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     7261 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     7321 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     7381 ctgaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     7441 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     7501 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     7561 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7621 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7681 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7741 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     7801 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7861 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7921 tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc
     7981 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8041 gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg
     8101 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8161 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8221 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8281 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8341 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8401 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8461 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc
     8521 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8581 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     8641 gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8701 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8761 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8821 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8881 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8941 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9001 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9061 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9121 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9181 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9241 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9301 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9361 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9421 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9481 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     9541 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9601 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9661 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9721 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9781 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9841 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9901 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9961 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10021 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10081 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10141 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10201 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10261 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10321 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10381 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10441 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10501 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10561 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10621 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10681 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10741 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10801 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    10861 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
    10921 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    10981 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
    11041 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
    11101 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
    11161 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    11221 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    11281 gccaagctg
//
