LOCUS       V034974                11067 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034974
VERSION     V034974
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5344
                     /label="PARP1(NM_001618)"
                     /note="PARP1(NM_001618)"
                     /gene="PARP1"
     CDS             5351..5416
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5460..5671
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5684..5952
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5987..6694
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6704..6757
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6758..7156
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    7163..7750
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7824..8057
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8129..8250
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8269..8404
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8442..8458)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8466..8482
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8490..8520)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8535..8556
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8844..9432)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9603..10463)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10464..10568)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11042..11058
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggcgga gtcttcggat aagctctatc gagtcgagta
     2341 cgccaagagc gggcgcgcct cttgcaagaa atgcagcgag agcatcccca aggactcgct
     2401 ccggatggcc atcatggtgc agtcgcccat gtttgatgga aaagtcccac actggtacca
     2461 cttctcctgc ttctggaagg tgggccactc catccggcac cctgacgttg aggtggatgg
     2521 gttctctgag cttcggtggg atgaccagca gaaagtcaag aagacagcgg aagctggagg
     2581 agtgacaggc aaaggccagg atggaattgg tagcaaggca gagaagactc tgggtgactt
     2641 tgcagcagag tatgccaagt ccaacagaag tacgtgcaag gggtgtatgg agaagataga
     2701 aaagggccag gtgcgcctgt ccaagaagat ggtggacccg gagaagccac agctaggcat
     2761 gattgaccgc tggtaccatc caggctgctt tgtcaagaac agggaggagc tgggtttccg
     2821 gcccgagtac agtgcgagtc agctcaaggg cttcagcctc cttgctacag aggataaaga
     2881 agccctgaag aagcagctcc caggagtcaa gagtgaagga aagagaaaag gcgatgaggt
     2941 ggatggagtg gatgaagtgg cgaagaagaa atctaaaaaa gaaaaagaca aggatagtaa
     3001 gcttgaaaaa gccctaaagg ctcagaacga cctgatctgg aacatcaagg acgagctaaa
     3061 gaaagtgtgt tcaactaatg acctgaagga gctactcatc ttcaacaagc agcaagtgcc
     3121 ttctggggag tcggcgatct tggaccgagt agctgatggc atggtgttcg gtgccctcct
     3181 tccctgcgag gaatgctcgg gtcagctggt cttcaagagc gatgcctatt actgcactgg
     3241 ggacgtcact gcctggacca agtgtatggt caagacacag acacccaacc ggaaggagtg
     3301 ggtaacccca aaggaattcc gagaaatctc ttacctcaag aaattgaagg ttaaaaaaca
     3361 ggaccgtata ttccccccag aaaccagcgc ctccgtggcg gccacgcctc cgccctccac
     3421 agcctcggct cctgctgctg tgaactcctc tgcttcagca gataagccat tatccaacat
     3481 gaagatcctg actctcggga agctgtcccg gaacaaggat gaagtgaagg ccatgattga
     3541 gaaactcggg gggaagttga cggggacggc caacaaggct tccctgtgca tcagcaccaa
     3601 aaaggaggtg gaaaagatga ataagaagat ggaggaagta aaggaagcca acatccgagt
     3661 tgtgtctgag gacttcctcc aggacgtctc cgcctccacc aagagccttc aggagttgtt
     3721 cttagcgcac atcttgtccc cttggggggc agaggtgaag gcagagcctg ttgaagttgt
     3781 ggccccaaga gggaagtcag gggctgcgct ctccaaaaaa agcaagggcc aggtcaagga
     3841 ggaaggtatc aacaaatctg aaaagagaat gaaattaact cttaaaggag gagcagctgt
     3901 ggatcctgat tctggactgg aacactctgc gcatgtcctg gagaaaggtg ggaaggtctt
     3961 cagtgccacc cttggcctgg tggacatcgt taaaggaacc aactcctact acaagctgca
     4021 gcttctggag gacgacaagg aaaacaggta ttggatattc aggtcctggg gccgtgtggg
     4081 tacggtgatc ggtagcaaca aactggaaca gatgccgtcc aaggaggatg ccattgagca
     4141 cttcatgaaa ttatatgaag aaaaaaccgg gaacgcttgg cactccaaaa atttcacgaa
     4201 gtatcccaaa aagttctacc ccctggagat tgactatggc caggatgaag aggcagtgaa
     4261 gaagctgaca gtaaatcctg gcaccaagtc caagctcccc aagccagttc aggacctcat
     4321 caagatgatc tttgatgtgg aaagtatgaa gaaagccatg gtggagtatg agatcgacct
     4381 tcagaagatg cccttgggga agctgagcaa aaggcagatc caggccgcat actccatcct
     4441 cagtgaggtc cagcaggcgg tgtctcaggg cagcagcgac tctcagatcc tggatctctc
     4501 aaatcgcttt tacaccctga tcccccacga ctttgggatg aagaagcctc cgctcctgaa
     4561 caatgcagac agtgtgcagg ccaaggtgga aatgcttgac aacctgctgg acatcgaggt
     4621 ggcctacagt ctgctcaggg gagggtctga tgatagcagc aaggatccca tcgatgtcaa
     4681 ctatgagaag ctcaaaactg acattaaggt ggttgacaga gattctgaag aagccgagat
     4741 catcaggaag tatgttaaga acactcatgc aaccacacac aatgcgtatg acttggaagt
     4801 catcgatatc tttaagatag agcgtgaagg cgaatgccag cgttacaagc cctttaagca
     4861 gcttcataac cgaagattgc tgtggcacgg gtccaggacc accaactttg ctgggatcct
     4921 gtcccagggt cttcggatag ccccgcctga agcgcccgtg acaggctaca tgtttggtaa
     4981 agggatctat ttcgctgaca tggtctccaa gagtgccaac tactgccata cgtctcaggg
     5041 agacccaata ggcttaatcc tgttgggaga agttgccctt ggaaacatgt atgaactgaa
     5101 gcacgcttca catatcagca agttacccaa gggcaagcac agtgtcaaag gtttgggcaa
     5161 aactacccct gatccttcag ctaacattag tctggatggt gtagacgttc ctcttgggac
     5221 cgggatttca tctggtgtga atgacacctc tctactatat aacgagtaca ttgtctatga
     5281 tattgctcag gtaaatctga agtatctgct gaaactgaaa ttcaatttta agacctccct
     5341 gtggggatcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     5401 ggatgacgat gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg
     5461 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     5521 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     5581 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     5641 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     5701 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     5761 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     5821 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     5881 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     5941 ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg tgagcaaggg
     6001 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     6061 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     6121 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     6181 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     6241 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     6301 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     6361 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     6421 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     6481 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     6541 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     6601 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     6661 ccactccacc ggcggcatgg acgagctgta caagggatct cgcgagggca gaggaagtct
     6721 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg gccaagcctt tgtctcaaga
     6781 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     6841 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     6901 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     6961 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     7021 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     7081 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     7141 tgtgtgggag ggctaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     7201 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7261 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7321 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7381 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7441 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7501 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     7561 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7621 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7681 tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttggcc
     7741 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7801 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
     7861 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7921 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7981 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8041 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8101 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     8161 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8221 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     8281 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8341 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8401 ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg
     8461 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8521 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8581 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8641 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8701 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8761 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8821 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8881 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8941 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9001 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9061 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9121 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9181 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9241 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     9301 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9361 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9421 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9481 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9541 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9601 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9661 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9721 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9781 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9841 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9901 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9961 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    10021 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    10081 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10141 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    10201 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    10261 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10321 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10381 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10441 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10501 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10561 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
    10621 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    10681 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
    10741 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    10801 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
    10861 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
    10921 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
    10981 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
    11041 tgtaaaacga cggccagtgc caagctg
//
