LOCUS       V023417                11315 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023417
VERSION     V023417
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5358
                     /label="PARP1(NM_001618)"
                     /note="PARP1(NM_001618)"
                     /gene="PARP1"
     CDS             5365..5430
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5474..5685
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5698..5966
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5995..6004
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6025..6687
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6757..6810
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6811..7410
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7411..7998
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8072..8305
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8377..8498
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8517..8652
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8690..8706)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8714..8730
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8738..8768)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8783..8804
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9092..9680)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9851..10711)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10712..10816)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11290..11306
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggagtcttc ggataagctc
     2341 tatcgagtcg agtacgccaa gagcgggcgc gcctcttgca agaaatgcag cgagagcatc
     2401 cccaaggact cgctccggat ggccatcatg gtgcagtcgc ccatgtttga tggaaaagtc
     2461 ccacactggt accacttctc ctgcttctgg aaggtgggcc actccatccg gcaccctgac
     2521 gttgaggtgg atgggttctc tgagcttcgg tgggatgacc agcagaaagt caagaagaca
     2581 gcggaagctg gaggagtgac aggcaaaggc caggatggaa ttggtagcaa ggcagagaag
     2641 actctgggtg actttgcagc agagtatgcc aagtccaaca gaagtacgtg caaggggtgt
     2701 atggagaaga tagaaaaggg ccaggtgcgc ctgtccaaga agatggtgga cccggagaag
     2761 ccacagctag gcatgattga ccgctggtac catccaggct gctttgtcaa gaacagggag
     2821 gagctgggtt tccggcccga gtacagtgcg agtcagctca agggcttcag cctccttgct
     2881 acagaggata aagaagccct gaagaagcag ctcccaggag tcaagagtga aggaaagaga
     2941 aaaggcgatg aggtggatgg agtggatgaa gtggcgaaga agaaatctaa aaaagaaaaa
     3001 gacaaggata gtaagcttga aaaagcccta aaggctcaga acgacctgat ctggaacatc
     3061 aaggacgagc taaagaaagt gtgttcaact aatgacctga aggagctact catcttcaac
     3121 aagcagcaag tgccttctgg ggagtcggcg atcttggacc gagtagctga tggcatggtg
     3181 ttcggtgccc tccttccctg cgaggaatgc tcgggtcagc tggtcttcaa gagcgatgcc
     3241 tattactgca ctggggacgt cactgcctgg accaagtgta tggtcaagac acagacaccc
     3301 aaccggaagg agtgggtaac cccaaaggaa ttccgagaaa tctcttacct caagaaattg
     3361 aaggttaaaa aacaggaccg tatattcccc ccagaaacca gcgcctccgt ggcggccacg
     3421 cctccgccct ccacagcctc ggctcctgct gctgtgaact cctctgcttc agcagataag
     3481 ccattatcca acatgaagat cctgactctc gggaagctgt cccggaacaa ggatgaagtg
     3541 aaggccatga ttgagaaact cggggggaag ttgacgggga cggccaacaa ggcttccctg
     3601 tgcatcagca ccaaaaagga ggtggaaaag atgaataaga agatggagga agtaaaggaa
     3661 gccaacatcc gagttgtgtc tgaggacttc ctccaggacg tctccgcctc caccaagagc
     3721 cttcaggagt tgttcttagc gcacatcttg tccccttggg gggcagaggt gaaggcagag
     3781 cctgttgaag ttgtggcccc aagagggaag tcaggggctg cgctctccaa aaaaagcaag
     3841 ggccaggtca aggaggaagg tatcaacaaa tctgaaaaga gaatgaaatt aactcttaaa
     3901 ggaggagcag ctgtggatcc tgattctgga ctggaacact ctgcgcatgt cctggagaaa
     3961 ggtgggaagg tcttcagtgc cacccttggc ctggtggaca tcgttaaagg aaccaactcc
     4021 tactacaagc tgcagcttct ggaggacgac aaggaaaaca ggtattggat attcaggtcc
     4081 tggggccgtg tgggtacggt gatcggtagc aacaaactgg aacagatgcc gtccaaggag
     4141 gatgccattg agcacttcat gaaattatat gaagaaaaaa ccgggaacgc ttggcactcc
     4201 aaaaatttca cgaagtatcc caaaaagttc taccccctgg agattgacta tggccaggat
     4261 gaagaggcag tgaagaagct gacagtaaat cctggcacca agtccaagct ccccaagcca
     4321 gttcaggacc tcatcaagat gatctttgat gtggaaagta tgaagaaagc catggtggag
     4381 tatgagatcg accttcagaa gatgcccttg gggaagctga gcaaaaggca gatccaggcc
     4441 gcatactcca tcctcagtga ggtccagcag gcggtgtctc agggcagcag cgactctcag
     4501 atcctggatc tctcaaatcg cttttacacc ctgatccccc acgactttgg gatgaagaag
     4561 cctccgctcc tgaacaatgc agacagtgtg caggccaagg tggaaatgct tgacaacctg
     4621 ctggacatcg aggtggccta cagtctgctc aggggagggt ctgatgatag cagcaaggat
     4681 cccatcgatg tcaactatga gaagctcaaa actgacatta aggtggttga cagagattct
     4741 gaagaagccg agatcatcag gaagtatgtt aagaacactc atgcaaccac acacaatgcg
     4801 tatgacttgg aagtcatcga tatctttaag atagagcgtg aaggcgaatg ccagcgttac
     4861 aagcccttta agcagcttca taaccgaaga ttgctgtggc acgggtccag gaccaccaac
     4921 tttgctggga tcctgtccca gggtcttcgg atagccccgc ctgaagcgcc cgtgacaggc
     4981 tacatgtttg gtaaagggat ctatttcgct gacatggtct ccaagagtgc caactactgc
     5041 catacgtctc agggagaccc aataggctta atcctgttgg gagaagttgc ccttggaaac
     5101 atgtatgaac tgaagcacgc ttcacatatc agcaagttac ccaagggcaa gcacagtgtc
     5161 aaaggtttgg gcaaaactac ccctgatcct tcagctaaca ttagtctgga tggtgtagac
     5221 gttcctcttg ggaccgggat ttcatctggt gtgaatgaca cctctctact atataacgag
     5281 tacattgtct atgatattgc tcaggtaaat ctgaagtatc tgctgaaact gaaattcaat
     5341 tttaagacct ccctgtgggg atccgactac aaagaccatg acggtgatta taaagatcat
     5401 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     5461 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     5521 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     5581 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     5641 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     5701 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5761 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5821 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5881 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5941 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     6001 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     6061 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     6121 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     6181 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     6241 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     6301 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     6361 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     6421 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     6481 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     6541 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     6601 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     6661 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     6721 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     6781 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     6841 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6901 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6961 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     7021 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     7081 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     7141 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     7201 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     7261 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     7321 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     7381 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     7441 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7501 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7561 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7621 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7681 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7741 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7801 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7861 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7921 ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc
     7981 ctttggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat
     8041 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa
     8101 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     8161 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8221 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8281 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8341 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     8401 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8461 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     8521 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8581 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8641 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt
     8701 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8761 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8821 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8881 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8941 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9001 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9061 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9121 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9181 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9241 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9301 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9361 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9421 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9481 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     9541 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9601 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9661 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9721 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9781 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9841 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9901 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9961 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10021 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10081 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10141 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10201 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10261 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10321 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10381 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10441 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10501 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10561 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10621 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10681 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10741 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10801 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    10861 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    10921 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    10981 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    11041 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    11101 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    11161 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    11221 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    11281 cacgacgttg taaaacgacg gccagtgcca agctg
//
